ROBO1 Antibody - #DF9877

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产品: ROBO1 抗体
货号: DF9877
描述: Rabbit polyclonal antibody to ROBO1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 180-250 kDa; 181kD(Calculated).
蛋白号: Q9Y6N7
RRID: AB_2843071

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Xenopus(%)
克隆:
Polyclonal
特异性:
ROBO1 Antibody detects endogenous levels of total ROBO1.
RRID:
AB_2843071
引用格式: Affinity Biosciences Cat# DF9877, RRID:AB_2843071.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Deleted in U twenty twenty; DUTT 1; DUTT1; FLJ21882; H Robo 1; H-Robo-1; hRobo 1; Robo 1; Robo1; ROBO1_HUMAN; Roundabout 1; Roundabout axon guidance receptor homolog 1; Roundabout homolog 1; Roundabout homolog1 precurser; Roundabout1; SAX 3; SAX3;

抗原和靶标

免疫原:
Uniprot:

Q9Y6N7(ROBO1_HUMAN) >>Visit HPA database.

基因/基因ID:
ROBO1(6091)
表达:
Q9Y6N7 ROBO1_HUMAN:

Widely expressed, with exception of kidney.

序列:
MKWKHVPFLVMISLLSLSPNHLFLAQLIPDPEDVERGNDHGTPIPTSDNDDNSLGYTGSRLRQEDFPPRIVEHPSDLIVSKGEPATLNCKAEGRPTPTIEWYKGGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRKSRPDEGVYVCVARNYLGEAVSHNASLEVAILRDDFRQNPSDVMVAVGEPAVMECQPPRGHPEPTISWKKDGSPLDDKDERITIRGGKLMITYTRKSDAGKYVCVGTNMVGERESEVAELTVLERPSFVKRPSNLAVTVDDSAEFKCEARGDPVPTVRWRKDDGELPKSRYEIRDDHTLKIRKVTAGDMGSYTCVAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQCEATGNPQPAIFWRREGSQNLLFSYQPPQSSSRFSVSQTGDLTITNVQRSDVGYYICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVATGSPVPTILWRKDGVLVSTQDSRIKQLENGVLQIRYAKLGDTGRYTCIASTPSGEATWSAYIEVQEFGVPVQPPRPTDPNLIPSAPSKPEVTDVSRNTVTLSWQPNLNSGATPTSYIIEAFSHASGSSWQTVAENVKTETSAIKGLKPNAIYLFLVRAANAYGISDPSQISDPVKTQDVLPTSQGVDHKQVQRELGNAVLHLHNPTVLSSSSIEVHWTVDQQSQYIQGYKILYRPSGANHGESDWLVFEVRTPAKNSVVIPDLRKGVNYEIKARPFFNEFQGADSEIKFAKTLEEAPSAPPQGVTVSKNDGNGTAILVSWQPPPEDTQNGMVQEYKVWCLGNETRYHINKTVDGSTFSVVIPFLVPGIRYSVEVAASTGAGSGVKSEPQFIQLDAHGNPVSPEDQVSLAQQISDVVKQPAFIAGIGAACWIILMVFSIWLYRHRKKRNGLTSTYAGIRKVPSFTFTPTVTYQRGGEAVSSGGRPGLLNISEPAAQPWLADTWPNTGNNHNDCSISCCTAGNGNSDSNLTTYSRPADCIANYNNQLDNKQTNLMLPESTVYGDVDLSNKINEMKTFNSPNLKDGRFVNPSGQPTPYATTQLIQSNLSNNMNNGSGDSGEKHWKPLGQQKQEVAPVQYNIVEQNKLNKDYRANDTVPPTIPYNQSYDQNTGGSYNSSDRGSSTSGSQGHKKGARTPKVPKQGGMNWADLLPPPPAHPPPHSNSEEYNISVDESYDQEMPCPVPPARMYLQQDELEEEEDERGPTPPVRGAASSPAAVSYSHQSTATLTPSPQEELQPMLQDCPEETGHMQHQPDRRRQPVSPPPPPRPISPPHTYGYISGPLVSDMDTDAPEEEEDEADMEVAKMQTRRLLLRGLEQTPASSVGDLESSVTGSMINGWGSASEEDNISSGRSSVSSSDGSFFTDADFAQAVAAAAEYAGLKVARRQMQDAAGRRHFHASQCPRPTSPVSTDSNMSAAVMQKTRPAKKLKHQPGHLRRETYTDDLPPPPVPPPAIKSPTAQSKTQLEVRPVVVPKLPSMDARTDRSSDRKGSSYKGREVLDGRQVVDMRTNPGDPREAQEQQNDGKGRGNKAAKRDLPPAKTHLIQEDILPYCRPTFPTSNNPRDPSSSSSMSSRGSGSRQREQANVGRRNIAEMQVLGGYERGEDNNEELEETES

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
88
Chicken
44
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y6N7 作为底物

Site PTM Type Enzyme
T42 Phosphorylation
S75 Phosphorylation
S80 Phosphorylation
K81 Ubiquitination
S251 Phosphorylation
S305 Phosphorylation
T321 Phosphorylation
T520 Phosphorylation
T660 O-Glycosylation
S661 O-Glycosylation
K743 Ubiquitination
K750 Ubiquitination
S855 Phosphorylation
S930 Phosphorylation
Y932 Phosphorylation
K937 Ubiquitination
S940 Phosphorylation
T942 Phosphorylation
T944 Phosphorylation
T946 Phosphorylation
T948 Phosphorylation
Y949 Phosphorylation
Y1019 Phosphorylation
K1026 Ubiquitination
Y1038 Phosphorylation P00519 (ABL1)
K1046 Ubiquitination
K1051 Ubiquitination
S1055 Phosphorylation
K1059 Ubiquitination
Y1073 Phosphorylation P00519 (ABL1)
T1075 Phosphorylation
S1094 Phosphorylation
K1100 Ubiquitination
K1106 Ubiquitination
Y1114 Phosphorylation P00519 (ABL1)
K1121 Ubiquitination
Y1126 Phosphorylation
R1237 Methylation
T1240 Phosphorylation
S1259 Phosphorylation
T1260 Phosphorylation
T1262 Phosphorylation
T1264 Phosphorylation
S1266 Phosphorylation
S1297 Phosphorylation
S1306 Phosphorylation
Y1311 Phosphorylation
Y1413 Phosphorylation
T1441 Phosphorylation
S1442 Phosphorylation
K1465 Ubiquitination
Y1476 Phosphorylation
S1492 Phosphorylation
T1494 Phosphorylation
S1497 Phosphorylation
S1528 Phosphorylation
Y1587 Phosphorylation
S1603 Phosphorylation
T1649 Phosphorylation

研究背景

功能:

Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development (By similarity).

翻译修饰:

Ubiquitinated. May be deubiquitinated by USP33.

细胞定位:

Cell membrane>Single-pass type I membrane protein. Cell projection>Axon. Endoplasmic reticulum-Golgi intermediate compartment membrane>Single-pass membrane protein.
Note: Detected at growth cones in thalamus neurons. Detected at growth cones in thalamus neurons (By similarity). PRRG4 prevents cell surface location and both colocalize in the Endoplasmic reticulum/Golgi adjacent to the cell nucleus (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed, with exception of kidney.

亚基结构:

Homodimer. Dimerization is mediated by the extracellular domain and is independent of SLIT liganding. Interacts with SLIT1 (By similarity). Interacts with SLIT2. Interacts with FLRT3. Interacts with MYO9B (via Rho-GAP domain).

蛋白家族:

Belongs to the immunoglobulin superfamily. ROBO family.

研究领域

· Organismal Systems > Development > Axon guidance.   (View pathway)

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