RPL22/L1 Antibody - #DF9870

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产品: RPL22/L1 抗体
货号: DF9870
描述: Rabbit polyclonal antibody to RPL22/L1
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q6P5R6 | P35268
RRID: AB_2843064

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
RPL22/L1 Antibody detects endogenous levels of total RPL22/L1.
RRID:
AB_2843064
引用格式: Affinity Biosciences Cat# DF9870, RRID:AB_2843064.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

60S ribosomal protein L22-like 1; RL22L_HUMAN; rpl22l1; 60S ribosomal protein L22; EAP; EBER associated protein; EBER-associated protein; Epstein Barr encoded RNA associated protein; Epstein Barr virus small RNA associated protein; Epstein-Barr virus small RNA-associated protein; HBP15; HBP15 L22; heparin binding protein 15; Heparin binding protein HBp15; Heparin-binding protein HBp15; ribosomal protein L22; RL22_HUMAN; rpl22;

抗原和靶标

免疫原:

A synthesized peptide derived from human RPL22/L1, corresponding to a region within the internal amino acids.

基因/基因ID:
RPL22(6146) | RPL22L1(200916)

研究领域

· Genetic Information Processing > Translation > Ribosome.

文献引用

1). Knockdown of ribosomal protein L22-like 1 arrests the cell cycle and promotes apoptosis in colorectal cancer. CytoJournal, 2024 (PubMed: 39737125) [IF=1.9]

Application: WB    Species: human    Sample: CRC cell

Figure 2: Knockdown of RPL22L1 and detection of its biological function. (a) Protein level of RPL22L1 in CRC cell lines and colonic epithelial cell line NCM460. (b-e) Western blot and qRT-PCR were conducted to identify the RPL22L1 expression downregulation in CRC cells. (f and g) Knockdown of RPL22L1 inhibited the proliferation of RKO and HCT116 cells. (h and i) Effect of RPL22L1 knockout and/or knockdown on the growth of CRC cells based on the DepMap portal. (j–k) Using wound healing experiments and (l) Transwell assays, the effect of RPL22L1 on RKO and HCT116 cell migration was identified. (n = 3; *P < 0.05, **P < 0.01, ***P < 0.001. CRC: Colorectal cancer, RPL22L1: Ribosomal Protein L22-Like 1, shCtrl: Control short hairpin RNA; siRPL22L1: RPL22L1-specific short hairpin RNA, qRTPCR: Quantitative real-time polymerase chain reaction.)

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