产品: 磷酸化 Tau (Ser404)[Ser721] 抗体
货号: AF3144
描述: Rabbit polyclonal antibody to Phospho-Tau (Ser404)[Ser721]
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: P10636
RRID: AB_2834579

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Tau (Ser404) Antibody detects endogenous levels of Tau only when phosphorylated at Ser721, which site historically referenced as Ser404.
RRID:
AB_2834579
引用格式: Affinity Biosciences Cat# AF3144, RRID:AB_2834579.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AI413597; AW045860; DDPAC; FLJ31424; FTDP 17; G protein beta1/gamma2 subunit interacting factor 1; MAPT; MAPTL; MGC134287; MGC138549; MGC156663; Microtubule associated protein tau; Microtubule associated protein tau isoform 4; Microtubule-associated protein tau; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament tau; Paired helical filament-tau; PHF tau; PHF-tau; PPND; PPP1R103; Protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; TAU; TAU_HUMAN; Tauopathy and respiratory failure, included;

抗原和靶标

免疫原:

A synthesized peptide derived from human Tau around the phosphorylation site of Ser404.

基因/基因ID:
描述:
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

文献引用

1). Dihydroartemisinin promotes tau O-GlcNAcylation and improves cognitive function in hTau transgenic mice. Progress in neuro-psychopharmacology & biological psychiatry, 2024 (PubMed: 39053763) [IF=5.3]

2). Maresin 1 alleviates sevoflurane-induced neuroinflammation in neonatal rats via JAK2/STAT3/IL-6 pathways. International Immunopharmacology, 2022 (PubMed: 35729840) [IF=4.8]

Application: WB    Species: Rat    Sample: hippocampus

Fig. 2. MaR1 decreased sevoflurane-induced hyperphosphorylation level of Tau at multi-sites. A-F Rats’ hippocampi were collected and analyzed by Western blotting using phosphorylated Tau antibodies (A) and quantitative analysis (B-F). One-way ANOVA, [pT205] F(3, 8) = 7.321, p = 0.0123; [pS214] F(3, 8) = 5.542, p = 0.0315; [pS262] F(3, 8) = 5.653, p = 0.0373; [pS396] F(3, 8) = 8.763, p = 0.0153; [pS404] F(3, 8) = 7.321, p = 0.0184. n = 3. * p < 0.05; # p < 0.05. Data were presented as mean ± SEM.

3). Exercise-induced upregulation of TRIM9 attenuates neuroinflammation in Alzheimer's disease-like rat. International immunopharmacology, 2025 (PubMed: 39580859) [IF=4.8]

4). MiR-29a efficiently suppresses the generation of reactive oxygen species and α-synuclein in a cellular model of Parkinson's disease by potentially targeting GSK-3β. European journal of pharmacology, 2024 (PubMed: 38685306) [IF=4.2]

5). 7,8-Dihydroxyflavone ameliorates cognitive impairment induced by repeated neonatal sevoflurane exposures in mice through increasing tau O-GlcNAcylation. Neuroscience letters, 2024 (PubMed: 37984484) [IF=2.5]

6). Therapeutic Mechanism of Kai Xin San on Alzheimer’s Disease Based on Network Pharmacology and Experimental Validation. Chinese Journal of Integrative Medicine, 2022 (PubMed: 36474082) [IF=2.2]

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