PRRX2 Antibody - #DF9729

Figure 2. Suppression of Ntmt1/Prrx2-SE activity by a pharmacological BRD4 inhibitor alleviates neuropathic pain sensitivities. a, The expression of BRD4 in dorsal horn on day 7 after CCI surgery was not changed; n ¼ 6 mice/group. b, Binding activity of BRD4 to three SS-SE clusters. ChIP-PCR with anti-BRD4 in the dorsal horn of Sham mice or CCI mice on day 7 after surgery; n ¼ 4 mice/group’ *p , 0.05, **p , 0.01 versus Sham. c, Binding activity of RNA polymerase II (RPII) to Ntmt1/Prrx2-SE clusters. ChIP-PCR with anti-RPII in the dorsal horn of Sham mice or CCI mice on day 7 after surgery; n ¼ 3 mice/group; ***p , 0.001 versus Sham1DMSO; # p , 0.05, ##p , 0.01 versus CCI1DMSO. d, Intrathecal injection of JQ1 7 d after CCI reduced the activity of Ntmt1/Prrx2-SE in ipsilateral dorsal horn 24 h after injection. SE activity was identified by H3K27ac-ChIP-qPCR. DMSO was used as the vehicle control; n ¼ 4 mice/group, *p , 0.05, **p , 0.01, ***p , 0.001 versus Sham1DMSO; # p , 0.05, ###p , 0.001, CCI1DMSO. e–j, Time course of the effect of intrathecal injection of JQ1 or vehicle control into CCI or sham male (e–g) and female (h–j) mice on the ipsilateral PWF to 0.07 g and 0.4 g von Frey filaments and on PWLs to heat stimuli; n ¼ 8 mice/group, ***p , 0.001, versus the Sham1DMSO group at the corresponding time points; # p , 0.05, ##p , 0.01, ###p , 0.001 versus CCI1DMSO at the corresponding time points; two-way ANOVA, post hoc Tukey’s tests. Red arrows indicate CCI or sham surgery. Blue arrows indicate JQ1 or vehicle control injection.