iNOS Antibody - #AF0199

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产品: iNOS 抗体
货号: AF0199
描述: Rabbit polyclonal antibody to iNOS
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
蛋白号: P35228
RRID: AB_2833391

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC: 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
iNOS Antibody detects endogenous levels of total iNOS.
RRID:
AB_2833391
引用格式: Affinity Biosciences Cat# AF0199, RRID:AB_2833391.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

HEP-NOS; Hepatocyte NOS; HEPNOS; inducible; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; iNOS; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible; Nitric oxide synthase 2 inducible macrophage; nitric oxide synthase 2A (inducible, hepatocytes); Nitric oxide synthase; Nitric oxide synthase inducible; nitric oxide synthase, macrophage; NOS 2; NOS; Nos II; NOS type II; nos2; NOS2_HUMAN; NOS2A; NOS2A, Inducible, Hepatocyte; Peptidyl-cysteine S-nitrosylase NOS2;

抗原和靶标

免疫原:

A synthesized peptide derived from human iNOS, corresponding to a region within C-terminal amino acids.

基因/基因ID:
NOS2(4843)
描述:
iNOS Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Belongs to the NOS family. Induced by endotoxins and cytokines. Induced by interferon gamma acting synergistically with lipopolysaccharide, tumor necrosis factor alpha or interleukin-1 beta. Homodimer. Binds SLC9A3R1. 2 isoforms of the human protein are produced by alternative splicing.

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Inhibiting Mitochondrial Damage for Efficient Treatment of Cerebral Ischemia-Reperfusion Injury Through Sequential Targeting Nanomedicine of Neuronal Mitochondria in Affected Brain Tissue. ADVANCED MATERIALS, 2024 [IF=27.4]
2). Efficient Therapy of Inflammatory Bowel Disease (IBD) with Highly Specific and Durable Targeted Ta2C Modified with Chondroitin Sulfate (TACS). Advanced Materials, 2023 (PubMed: 37224059) [IF=27.4]

Application: WB    Species: Mouse    Sample: RAW264.7 cells

Figure 7.TACS relieves inflammation and restores intestinal barrier function. B)WB analysis of COX-2 and iNOS proteins expression in RAW264.7 cells with different treatments.
3). A novel Nanocellulose-Gelatin-AS-IV external stent resists EndMT by activating autophagy to prevent restenosis of grafts. Bioactive Materials, 2023 (PubMed: 36330163) [IF=18.9]
4). Apoptotic extracellular vesicles restore homeostasis of the articular microenvironment for the treatment of rheumatoid arthritis. Bioactive materials, 2024 (PubMed: 38469201) [IF=18.9]

Application: IF/ICC    Species: Mouse    Sample: chondrocytes

Fig. 2. Repolarization of macrophages from M1 toward M2 phenotype by apoEVs. (a) Representative images showing the cellular uptake of DiD-labeled apoEVs (red) by macrophages. (Scale bar, 20 μm) (b) FCM analysis of CD206 and CCR7 expression. (c) Representative images of iNOS (red) in macrophages. (Scale bar, 25 μm) (d) Representative images of ROS (green) production by macrophages. (Scale bar, 100 μm) (e) Concentration of NO in the culture medium by Griess assay. (n = 5) (f) Concentration of TNF-α, TGF-β1, and Arg1 in the culture medium by ELISA. (n = 3) (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
5). Controlled TPCA-1 delivery engineers a pro-tenogenic niche to initiate tendon regeneration by targeting IKKβ/NF-κB signaling. Bioactive materials, 2024 (PubMed: 39512422) [IF=18.9]

Application: IF/ICC    Species: Rat    Sample:

Fig. 9. The controlled release of TPCA-1 promotes tenogenic differentiation of hPDLSCs for tendon repair in rats. (A) H&E staining and MT staining of the repaired tendons in the Ctrl or TPCA-1 groups. Black arrows indicated the cells with the characteristics of inflammatory cells at 4 weeks. Low magnification: scale bars = 1 mm; high magnification: scale bars = 40 μm. (B) Total histology scores of repaired tendons in the Ctrl and TPCA-1 groups from H&E staining images at 4 weeks after injury (n = 8 biological replicates). A lower score indicates a more mature tendon tissue. (C) Quantitative analysis of collagen density in the repaired tendons of the Ctrl and TPCA-1 groups from MT staining images (n = 8 biological replicates). (D–E) Representative images of IF staining for SCX (D) and TNMD (E) in the repaired tendons of the Ctrl and TPCA-1 groups at 4 weeks after injury. Scale bars = 50 μm. (F–G) Quantitative analysis of IF staining for SCX (F) and TNMD (G). (H) Representative images and quantitative analysis of IHC staining for MKX at 4 weeks after injury. Scale bars = 20 μm. (I) Quantitative analysis of intensity parameters via gait analysis at 2 weeks after injury (n = 5 biological replicates). (J) Typical strain-stress curves of repaired tendons. (K) Tensile modulus, max stress, and max force of repaired tendons at 4 weeks after injury (n = 5 biological replicates). Results were shown as means ± SEM. n = 6 randomly-selected microscopic images for quantitative analysis for Fig. 9F–H. P values were determined by the Student's t-test (D–F) and Mann-Whiteney test (G). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
6). Enhancing Tendon Regeneration: Investigating the Impact of Topography on the Secretome of Adipose-Derived Stem Cells. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 40091553) [IF=15.1]
7). Regulation of immune microenvironments by polyetheretherketone surface topography for improving osseointegration. Journal of nanobiotechnology, 2025 (PubMed: 40069791) [IF=12.6]
8). Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction. Science advances, 2024 (PubMed: 38657071) [IF=11.7]
9). Ganoderma lucidum polysaccharide modulates gut microbiota and immune cell function to inhibit inflammation and tumorigenesis in colon. Carbohydrate Polymers, 2021 (PubMed: 34119183) [IF=10.7]
10). Plasma Membrane-Derived Biomimetic Apoptotic Nanovesicles Targeting Inflammation and Cartilage Degeneration for Osteoarthritis. Small methods, 2024 (PubMed: 39036830) [IF=10.7]
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