产品: Cytokeratin 10 抗体
货号: AF0197
描述: Rabbit polyclonal antibody to Cytokeratin 10
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 59kDa; 59kD(Calculated).
蛋白号: P13645
RRID: AB_2833390

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC: 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(89%), Bovine(95%), Sheep(95%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Cytokeratin 10 Antibody detects endogenous levels of total Cytokeratin 10.
RRID:
AB_2833390
引用格式: Affinity Biosciences Cat# AF0197, RRID:AB_2833390.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BCIE; BIE; CK 10; CK-10; Cytokeratin-10; EHK; K10; K1C10_HUMAN; Keratin 10; Keratin 10 type I; Keratin; Keratin type i cytoskeletal 10; Keratin type I cytoskeletal 59 kDa; Keratin-10; Keratin10; KPP; KRT10; type I cytoskeletal 10;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P13645 K1C10_HUMAN:

Seen in all suprabasal cell layers including stratum corneum. Expressed on the surface of lung cell lines (PubMed:19627498).

描述:
K10 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
序列:
MSVRYSSSKHYSSSRSGGGGGGGGCGGGGGVSSLRISSSKGSLGGGFSSGGFSGGSFSRGSSGGGCFGGSSGGYGGLGGFGGGSFRGSYGSSSFGGSYGGIFGGGSFGGGSFGGGSFGGGGFGGGGFGGGFGGGFGGDGGLLSGNEKVTMQNLNDRLASYLDKVRALEESNYELEGKIKEWYEKHGNSHQGEPRDYSKYYKTIDDLKNQILNLTTDNANILLQIDNARLAADDFRLKYENEVALRQSVEADINGLRRVLDELTLTKADLEMQIESLTEELAYLKKNHEEEMKDLRNVSTGDVNVEMNAAPGVDLTQLLNNMRSQYEQLAEQNRKDAEAWFNEKSKELTTEIDNNIEQISSYKSEITELRRNVQALEIELQSQLALKQSLEASLAETEGRYCVQLSQIQAQISALEEQLQQIRAETECQNTEYQQLLDIKIRLENEIQTYRSLLEGEGSSGGGGRGGGSFGGGYGGGSSGGGSSGGGHGGGHGGSSGGGYGGGSSGGGSSGGGYGGGSSSGGHGGSSSGGYGGGSSGGGGGGYGGGSSGGGSSSGGGYGGGSSSGGHKSSSSGSVGESSSKGPRY

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Rabbit
100
Dog
100
Bovine
95
Sheep
95
Pig
89
Chicken
58
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P13645 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
Y11 Phosphorylation
S13 Phosphorylation
S14 Phosphorylation
S16 Phosphorylation
S38 Phosphorylation
S39 Phosphorylation
S42 Phosphorylation
S53 Phosphorylation
S56 Phosphorylation
S62 Phosphorylation
S71 Phosphorylation
S159 Phosphorylation
Y160 Phosphorylation
K163 Acetylation
K163 Ubiquitination
S170 Phosphorylation
Y172 Phosphorylation
S188 Phosphorylation
Y199 Phosphorylation
Y238 Phosphorylation
T277 Phosphorylation
Y282 Phosphorylation
K284 Sumoylation
K284 Ubiquitination
S298 Phosphorylation
Y325 Phosphorylation
K343 Ubiquitination
S344 Phosphorylation
T430 Phosphorylation
S458 Phosphorylation
S459 Phosphorylation
S535 Phosphorylation
S546 Phosphorylation
Y557 Phosphorylation
S568 Phosphorylation
S569 Phosphorylation
S571 Phosphorylation
S573 Phosphorylation
S577 Phosphorylation
S578 Phosphorylation
S579 Phosphorylation
Y584 Phosphorylation

研究背景

功能:

Plays a role in the establishment of the epidermal barrier on plantar skin.

(Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization.

(Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.

细胞定位:

Secreted>Extracellular space. Cell surface.
Note: Localized on the surface of desquamated nasal epithelial cells (PubMed:12427098). Localized on the surface of lung cell lines (PubMed:19627498).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Seen in all suprabasal cell layers including stratum corneum. Expressed on the surface of lung cell lines.

亚基结构:

Heterotetramer of two type I and two type II keratins. Heterodimer with KRT1. Two heterodimers of KRT1 and KRT10 form a heterotetramer. The KRT10 subunit in the heterotetramer is probably disulfide-linked (Probable).

(Microbial infection) Interacts (via C-terminal tail domain) with the S.aureus clumping factor, clfB; this interaction probably mediates S.aureus attachment to the keratinized squamous epithelial cells from the nasal cavity.

(Microbial infection) Interacts (via the C-terminal tail domain) with S.pneumoniae serine-rich repeat protein PsrP; this interaction probably mediates S.pneumoniae adherence to lung tissue and subsequent pathogenesis. Neither protein has to be glycosylated for the interaction to occur.

蛋白家族:

Belongs to the intermediate filament family.

研究领域

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

文献引用

1). Wang S et al. Stem cell membrane-coated isotretinoin for acne treatment. JOURNAL OF NANOBIOTECHNOLOGY 2020 Jul 28;18(1):106 (PubMed: 32723398) [IF=10.2]

2). Yao Q et al. Water-responsive gel extends drug retention and facilitates skin penetration for curcumin topical delivery against psoriasis. Asian Journal of Pharmaceutical Sciences 2023 Mar;18(100782) (PubMed: 36845839) [IF=10.2]

3). Chen R et al. Alantolactone-loaded chitosan/hyaluronic acid nanoparticles suppress psoriasis by deactivating STAT3 pathway and restricting immune cell recruitment. Asian Journal of Pharmaceutical Sciences 2022 Mar;17(2):268-283. (PubMed: 35582636) [IF=10.2]

Application: IHC    Species: Mice    Sample: skin tissue

Fig. 6 CHALT suppresses keratinocyte proliferation in IMQ-induced skin. The skin was evaluated by (A) Masson's trichrome staining, and quantitative analysis was shown (D). Immunohistochemical staining of (B) CK10 and (C) Ki 67 was performed, and quantitative analysis was shown in (E) and (F), respectively. Scale bar: 100 µM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, indicate statistical difference compared to model group or between groups. ns indicates no statistical significance. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Application: IHC    Species: mouse    Sample: skin

Fig. 6|CHALT suppresses keratinocyte proliferation in IMQ-induced skin. The skin was evaluated by (A) Masson’s trichrome staining, and quantitative analysis was shown (D). Immunohistochemical staining of (B) CK10 and (C) Ki 67 was performed,and quantitative analysis was shown in (E) and (F), respectively.

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