LRP4 Antibody - #DF9610

Fig. 4 MicroRNA (miR)-136-5p activates the Wnt/β-catenin pathway by targeting low-density lipoprotein receptor related protein 4 (LRP4). a) miR-136-5p binding sites in the LRP4 3’-untranslated region (3’-UTR) predicted by the TargetScan website, and binding of miR-136-5p to LRP4 confirmed by dual luciferase reporter gene assay in HEK293 cells; b) reverse transcription quantitative polymerase chain reaction (RT-qPCR) detection of the transfection efficiency of miR-136-5p mimic in MC3T3-E1 cells; c) RT-qPCR detection of the transfection efficiency of miR-136-5p inhibitor in MC3T3-E1 cells; d) Western blot detection of the protein expression of LRP4 in MC3T3-E1 cells with miR-136-5p overexpression or silencing; e) RT-qPCR detection of the expression of miR-136-5p in MC3T3-E1 cells transfected with miR-136-5p mimic, oe-LRP4, or both; f) Western blot detection of the expression of LRP4 and Wnt/β-catenin pathway related proteins in MC3T3-E1 cells transfected with miR-136-5p mimic, oe-LRP4, or both; g) RT-qPCR detected the expression of miR-136-5p in MC3T3-E1 cells treated with BMSC-Exos or in combination with miR-136-5p inhibitor; h) Western blot detection of the expression of LRP4 and Wnt/β-catenin pathway related proteins in MC3T3-E1 cells treated with BMSC-Exos or in combination with miR-136-5p inhibitor. # indicates p < 0.05, and non-significant (NS) represents p > 0.05. Measurement data were presented as mean and standard deviation. Unpaired t-test was used for data comparison between two groups while data comparisons among multiple groups were performed using one-way analysis of variance (ANOVA) with Tukey’s post hoc test. Data comparison among groups at different timepoints was performed by repeated measurement ANOVA with Bonferroni’s post hoc test. The experiment was repeated three times. MUT, mutant; PBS, phosphate-buffered saline; WT, wild type.

FIGURE 6 Theexpressionofneuronalsignaling-transduction proteinsinhippocampusofDREratsat9weeksafterSE.(a)Western blottingbands;(b)barographsofexpressionlevels.*p<.05versusthe