Phospho-Ret (Tyr1062) Antibody - #AF3120

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产品: 磷酸化 Ret (Tyr1062) 抗体
货号: AF3120
描述: Rabbit polyclonal antibody to Phospho-Ret (Tyr1062)
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Xenopus
蛋白号: P07949
RRID: AB_2834555

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   规格 价格 库存
 50ul RMB¥ 1300 现货
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Ret (Tyr1062) Antibody detects endogenous levels of Ret only when phosphorylated at Tyrosine 1062.
RRID:
AB_2834555
引用格式: Affinity Biosciences Cat# AF3120, RRID:AB_2834555.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C ret; Cadherin family member 12; Cadherin related family member 16; CDHF 12; CDHF12; CDHR16; ELKS Fusion gene; HSCR 1; HSCR1; Hydroxyaryl protein kinase; MEN2A; MEN2B; MTC 1; MTC1; Multiple endocrine neoplasia and medullary thyroid carcinoma 1; Oncogene RET; Proto oncogene tyrosine protein kinase receptor ret; Proto-oncogene c-Ret; Proto-oncogene tyrosine-protein kinase receptor ret; PTC; RET; RET ELE1; Ret Proto oncogene; RET transforming sequence; RET_HUMAN; RET51; RET9; tyrosine-protein kinase receptor ret;

抗原和靶标

免疫原:

A synthesized peptide derived from human Ret around the phosphorylation site of Tyr1062.

基因/基因ID:
RET(5979)
描述:
This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement.

研究领域

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Thyroid cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

文献引用

1). Growth differentiation factor-15 overexpression promotes cell proliferation and predicts poor prognosis in cerebral lower-grade gliomas correlated with hypoxia and glycolysis signature. Life Sciences, 2022 (PubMed: 35588865) [IF=5.2]
2). Recurrent non-severe hypoglycemia aggravates cognitive decline in diabetes and induces mitochondrial dysfunction in cultured astrocytes. Molecular and Cellular Endocrinology, 2021 (PubMed: 33545179) [IF=3.8]

Application: WB    Species: mice    Sample: cortex tissue

Fig. 3. Recurrent non-severe hypoglycemia (RH) inhibits brain-derived neurotrophic factor (BDNF) and glial cell derived neurotrophic factor (GDNF) signaling in diabetic mice. (A) The expression of BDNF/tropomyosin (t)-receptor (r)-kinase (k) B (TrkB) signaling and GDNF/GDNF family receptor alpha-1 (GFRa1)/receptor tyrosine kinase (Ret) signaling in mice cerebral cortex, measured using western blotting. (B–G) Relative protein levels of BDNF, TrkB, p-TrkB, GDNF, GFRa1, Ret, and p-Ret (Tyr1062), as determined by ImageJ. *P < 0.05 vs. normal control (NC), **P < 0.01 vs. NC, #P < 0.05 vs. diabetes mellitus (DM), ##P < 0.05 vs. DM.
3). CCL2/CCR2 Axis Promotes the Progression of Salivary Adenoid Cystic Carcinoma via Recruiting and Reprogramming the Tumor-Associated Macrophages. Frontiers in Oncology, 2019 (PubMed: 31024838) [IF=3.5]

Application: WB    Species: human    Sample: SACC-83 cells

FIGURE 5 | TAMs promoted the proliferation, migration, and invasion of SACC cells via the GDNF/p-RET pathway. The TAMs conditioned media (TAMs-CM) was used to stimulate the SACC-83 cells. The macrophages conditioned media (macrophages-CM) served as the control. The CCR2 antagonist (RS504393, 50 ng/mL),GDNF NAb (2µg/mL), and RET antagonist (PYP-1, 5µM) were used. (A–C) The expression of p-RET and RET in SACC-83 cells were evaluated by western blot.

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