产品: 磷酸化 Lyn (Tyr508) 抗体
货号: AF3119
描述: Rabbit polyclonal antibody to Phospho-Lyn (Tyr508)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 53,56kDa; 59kD(Calculated).
蛋白号: P07948
RRID: AB_2834554

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(89%)
克隆:
Polyclonal
特异性:
Phospho-Lyn (Tyr508) Antibody detects endogenous levels of Lyn only when phosphorylated at Tyrosine 507.
RRID:
AB_2834554
引用格式: Affinity Biosciences Cat# AF3119, RRID:AB_2834554.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AA407514; EC 2.7.10.2; FLJ26625; Hck 2; JTK 8; JTK8; Lck/Yes related novel protein tyrosine kinase; LYN; LYN proto oncogene, Src family tyrosine kinase; LYN_HUMAN; ONCOGENE LYN; p53Lyn; p56Lyn; Tyrosine protein kinase LYN; Tyrosine-protein kinase Lyn; V yes 1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v yes 1) related oncogene homolog;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P07948 LYN_HUMAN:

Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.

描述:
Lyn a tyrosine kinase of the Src family that plays a role in B cell antigen-receptor signaling.
序列:
MGCIKSKGKDSLSDDGVDLKTQPVRNTERTIYVRDPTSNKQQRPVPESQLLPGQRFQTKDPEEQGDIVVALYPYDGIHPDDLSFKKGEKMKVLEEHGEWWKAKSLLTKKEGFIPSNYVAKLNTLETEEWFFKDITRKDAERQLLAPGNSAGAFLIRESETLKGSFSLSVRDFDPVHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKHYQKQADGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKRLGAGQFGEVWMGYYNNSTKVAVKTLKPGTMSVQAFLEEANLMKTLQHDKLVRLYAVVTREEPIYIITEYMAKGSLLDFLKSDEGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCFTIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMTALSQGYRMPRVENCPDELYDIMKMCWKEKAEERPTFDYLQSVLDDFYTATEGQYQQQP

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Chicken
100
Rabbit
100
Xenopus
89
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07948 作为底物

Site PTM Type Enzyme
G2 Myristoylation
K9 Ubiquitination
S11 Phosphorylation
S13 Phosphorylation
K20 Ubiquitination
T30 Phosphorylation
Y32 Phosphorylation P00533 (EGFR)
K40 Ubiquitination
T58 Phosphorylation
K59 Ubiquitination
K85 Ubiquitination
K101 Ubiquitination
K109 Ubiquitination
S115 Phosphorylation
Y117 Phosphorylation
K132 Ubiquitination
T135 Phosphorylation
S149 Phosphorylation
S158 Phosphorylation
S164 Phosphorylation
S166 Phosphorylation
K181 Ubiquitination
S187 Phosphorylation
Y193 Phosphorylation
Y194 Phosphorylation
K213 Ubiquitination
K224 Ubiquitination
S228 Phosphorylation
S246 Phosphorylation
Y265 Phosphorylation
Y266 Phosphorylation
S269 Phosphorylation
T276 Phosphorylation
T281 Phosphorylation
S283 Phosphorylation
T296 Phosphorylation
Y306 Phosphorylation
Y316 Phosphorylation
T319 Phosphorylation
Y321 Phosphorylation
S326 Phosphorylation
K332 Ubiquitination
K338 Ubiquitination
Y397 Phosphorylation P07948 (LYN)
T398 Phosphorylation
K404 Ubiquitination
T438 Phosphorylation
Y439 Phosphorylation
T448 Phosphorylation
Y460 Phosphorylation
Y473 Phosphorylation
K477 Ubiquitination
Y501 Phosphorylation
T502 Phosphorylation
T504 Phosphorylation
Y508 Phosphorylation P07948 (LYN) , P41240 (CSK) , P42679 (MATK)

翻译修饰 - P07948 作为激酶

Substrate Site Source
O14920 (IKBKB) Y188 Uniprot
O14920 (IKBKB) Y199 Uniprot
O15492 (RGS16) Y168 Uniprot
O15492 (RGS16) Y177 Uniprot
O43823 (AKAP8) Y51 Uniprot
O43823 (AKAP8) Y53 Uniprot
O43823 (AKAP8) Y80 Uniprot
O43823 (AKAP8) Y146 Uniprot
O43823 (AKAP8) Y150 Uniprot
O43823 (AKAP8) Y152 Uniprot
O43823 (AKAP8) Y154 Uniprot
O43823 (AKAP8) Y170 Uniprot
O43823 (AKAP8) Y311 Uniprot
O43823 (AKAP8) Y436 Uniprot
O43823 (AKAP8) Y539 Uniprot
O60496 (DOK2) Y271 Uniprot
O60496 (DOK2) Y299 Uniprot
O60496 (DOK2) Y345 Uniprot
O60711 (LPXN) Y72 Uniprot
P00519 (ABL1) Y70 Uniprot
P00519 (ABL1) Y115 Uniprot
P00519 (ABL1) Y128 Uniprot
P00519 (ABL1) Y139 Uniprot
P00519 (ABL1) Y172 Uniprot
P00519 (ABL1) Y185 Uniprot
P00519 (ABL1) Y215 Uniprot
P00519 (ABL1) Y226 Uniprot
P00519 (ABL1) Y393 Uniprot
P02730 (SLC4A1) Y8 Uniprot
P02730 (SLC4A1) Y21 Uniprot
P02730 (SLC4A1) Y359 Uniprot
P02730 (SLC4A1) Y904 Uniprot
P06493-2 (CDK1) Y15 Uniprot
P07948 (LYN) Y397 Uniprot
P07948 (LYN) Y508 Uniprot
P11137 (MAP2) Y67 Uniprot
P11274 (BCR) Y177 Uniprot
P11802 (CDK4) Y17 Uniprot
P11912-2 (CD79A) Y150 Uniprot
P11912 (CD79A) Y188 Uniprot
P12318-2 (FCGR2A) Y280 Uniprot
P12318 (FCGR2A) Y281 Uniprot
P12318-2 (FCGR2A) Y287 Uniprot
P12318 (FCGR2A) Y288 Uniprot
P12318-2 (FCGR2A) Y303 Uniprot
P12318 (FCGR2A) Y304 Uniprot
P13569 (CFTR) Y512 Uniprot
P13569 (CFTR) Y515 Uniprot
P13569 (CFTR) Y517 Uniprot
P14317-1 (HCLS1) Y222 Uniprot
P14317 (HCLS1) Y378 Uniprot
P14317 (HCLS1) Y397 Uniprot
P15391-1 (CD19) Y409 Uniprot
P15391 (CD19) Y500 Uniprot
P15391 (CD19) Y531 Uniprot
P15941 (MUC1) Y1229 Uniprot
P16885 (PLCG2) Y743 Uniprot
P16885 (PLCG2) Y753 Uniprot
P16885 (PLCG2) Y759 Uniprot
P19174-1 (PLCG1) Y771 Uniprot
P19174-2 (PLCG1) Y783 Uniprot
P19235 (EPOR) Y368 Uniprot
P21854 (CD72) Y7 Uniprot
P21854 (CD72) Y39 Uniprot
P22681 (CBL) Y371 Uniprot
P24941 (CDK2) Y15 Uniprot
P25490 (YY1) Y8 Uniprot
P25490 (YY1) Y254 Uniprot
P25490 (YY1) Y383 Uniprot
P29350 (PTPN6) Y536 Uniprot
P29350 (PTPN6) Y564 Uniprot
P30419 (NMT1) Y117 Uniprot
P30419 (NMT1) Y180 Uniprot
P31994-5 (FCGR2B) Y272 Uniprot
P31994-2 (FCGR2B) Y273 Uniprot
P31994-4 (FCGR2B) Y291 Uniprot
P31994-1 (FCGR2B) Y292 Uniprot
P31995 (FCGR2C) Y310 Uniprot
P32927 (CSF2RB) Y466 Uniprot
P32927 (CSF2RB) Y468 Uniprot
P32927 (CSF2RB) Y822 Uniprot
P32927 (CSF2RB) Y882 Uniprot
P33993 (MCM7) Y600 Uniprot
P35354 (PTGS2) Y120 Uniprot
P37840-1 (SNCA) Y125 Uniprot
P40259-2 (CD79B) Y92 Uniprot
P40259-2 (CD79B) Y103 Uniprot
P40259 (CD79B) Y196 Uniprot
P40259 (CD79B) Y207 Uniprot
P42229 (STAT5A) Y694 Uniprot
P42680 (TEC) Y519 Uniprot
P42768 (WAS) Y291 Uniprot
P43405-1 (SYK) Y131 Uniprot
P43405 (SYK) Y323 Uniprot
P43405 (SYK) Y348 Uniprot
P43405 (SYK) Y352 Uniprot
P46527 (CDKN1B) Y88 Uniprot
P60484 (PTEN) Y240 Uniprot
P68400 (CSNK2A1) Y255 Uniprot
Q05655-1 (PRKCD) Y52 Uniprot
Q05655-1 (PRKCD) Y64 Uniprot
Q05655-1 (PRKCD) Y155 Uniprot
Q05655-1 (PRKCD) Y187 Uniprot
Q05655 (PRKCD) Y313 Uniprot
Q05655 (PRKCD) Y334 Uniprot
Q05655-1 (PRKCD) Y567 Uniprot
Q06187 (BTK) Y223 Uniprot
Q06187 (BTK) Y551 Uniprot
Q12972-3 (PPP1R8) Y40 Uniprot
Q12972-3 (PPP1R8) Y111 Uniprot
Q12972-2 (PPP1R8) Y122 Uniprot
Q12972-2 (PPP1R8) Y193 Uniprot
Q12972 (PPP1R8) Y264 Uniprot
Q12972 (PPP1R8) Y335 Uniprot
Q13263 (TRIM28) Y449 Uniprot
Q13263 (TRIM28) Y458 Uniprot
Q13263 (TRIM28) Y517 Uniprot
Q13291 (SLAMF1) Y327 Uniprot
Q14790 (CASP8) Y380 Uniprot
Q14790 (CASP8) Y448 Uniprot
Q8N6F7 (GCSAM) Y107 Uniprot
Q8N6F7 (GCSAM) Y128 Uniprot
Q92918-1 (MAP4K1) Y381 Uniprot
Q9GZY6 (LAT2) Y95 Uniprot
Q9GZY6 (LAT2) Y110 Uniprot
Q9GZY6 (LAT2) Y118 Uniprot
Q9GZY6 (LAT2) Y119 Uniprot
Q9GZY6 (LAT2) Y136 Uniprot
Q9H792 (PEAK1) Y635 Uniprot
Q9HBA0 (TRPV4) Y253 Uniprot
Q9NWQ8 (PAG1) Y105 Uniprot
Q9UN19 (DAPP1) Y139 Uniprot
Q9Y6Y9 (LY96) Y22 Uniprot
Q9Y6Y9 (LY96) Y131 Uniprot

研究背景

功能:

Non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. Functions primarily as negative regulator, but can also function as activator, depending on the context. Required for the initiation of the B-cell response, but also for its down-regulation and termination. Plays an important role in the regulation of B-cell differentiation, proliferation, survival and apoptosis, and is important for immune self-tolerance. Acts downstream of several immune receptors, including the B-cell receptor, CD79A, CD79B, CD5, CD19, CD22, FCER1, FCGR2, FCGR1A, TLR2 and TLR4. Plays a role in the inflammatory response to bacterial lipopolysaccharide. Mediates the responses to cytokines and growth factors in hematopoietic progenitors, platelets, erythrocytes, and in mature myeloid cells, such as dendritic cells, neutrophils and eosinophils. Acts downstream of EPOR, KIT, MPL, the chemokine receptor CXCR4, as well as the receptors for IL3, IL5 and CSF2. Plays an important role in integrin signaling. Regulates cell proliferation, survival, differentiation, migration, adhesion, degranulation, and cytokine release. Down-regulates signaling pathways by phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIM), that then serve as binding sites for phosphatases, such as PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1, that modulate signaling by dephosphorylation of kinases and their substrates. Phosphorylates LIME1 in response to CD22 activation. Phosphorylates BTK, CBL, CD5, CD19, CD72, CD79A, CD79B, CSF2RB, DOK1, HCLS1, LILRB3/PIR-B, MS4A2/FCER1B, SYK and TEC. Promotes phosphorylation of SIRPA, PTPN6/SHP-1, PTPN11/SHP-2 and INPP5D/SHIP-1. Mediates phosphorylation of the BCR-ABL fusion protein. Required for rapid phosphorylation of FER in response to FCER1 activation. Mediates KIT phosphorylation. Acts as an effector of EPOR (erythropoietin receptor) in controlling KIT expression and may play a role in erythroid differentiation during the switch between proliferation and maturation. Depending on the context, activates or inhibits several signaling cascades. Regulates phosphatidylinositol 3-kinase activity and AKT1 activation. Regulates activation of the MAP kinase signaling cascade, including activation of MAP2K1/MEK1, MAPK1/ERK2, MAPK3/ERK1, MAPK8/JNK1 and MAPK9/JNK2. Mediates activation of STAT5A and/or STAT5B. Phosphorylates LPXN on 'Tyr-72'. Kinase activity facilitates TLR4-TLR6 heterodimerization and signal initiation. Phosphorylates SCIMP on 'Tyr-107'; this enhances binding of SCIMP to TLR4, promoting the phosphorylation of TLR4, and a selective cytokine response to lipopolysaccharide in macrophages (By similarity). Phosphorylates CLNK (By similarity).

翻译修饰:

Ubiquitinated by CBL, leading to its degradation. Ubiquitination is SH3-dependent.

Autophosphorylated. Phosphorylated on tyrosine residues in response to KIT signaling. Phosphorylation at Tyr-397 is required for optimal activity. Phosphorylation at Tyr-508 inhibits kinase activity. Phosphorylated at Tyr-508 by CSK. Dephosphorylated by PTPRC/CD45. Becomes rapidly phosphorylated upon activation of the B-cell receptor and the immunoglobulin receptor FCGR1A.

细胞定位:

Cell membrane. Nucleus. Cytoplasm. Cytoplasm>Perinuclear region. Golgi apparatus. Membrane>Lipid-anchor.
Note: Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. Detected on plasma membrane lipid rafts.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in monocytes (at protein level). Detected in placenta, and in fetal brain, lung, liver and kidney. Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.

亚基结构:

Interacts with TEC. Interacts (via SH2 domain) with FLT3 (tyrosine phosphorylated). Interacts with LIME1 and with CD79A upon activation of the B-cell antigen receptor. Interacts with the B-cell receptor complex. Interacts with phosphorylated THEMIS2. Interacts with EPOR. Interacts with MS4A2/FCER1B. Interaction (via the SH2 and SH3 domains) with MUC1 is stimulated by IL7 and the subsequent phosphorylation increases the binding between MUC1 and CTNNB1/beta-catenin. Interacts with ADAM15. Interacts with NDFIP2 and more weakly with NDFIP1. Interacts with FASLG. Interacts with KIT. Interacts with HCLS1. Interacts with FCGR2B. Interacts with FCGR1A; the interaction may be indirect. Interacts with CD19, CD22, CD79A and CD79B. Interacts (via SH3 domain) with CBLC, PPP1R15A and PDE4A. Interacts with TGFB1I1. Interacts (via SH3 domain) with PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase; this interaction enhances phosphatidylinositol 3-kinase activity. Interacts with CSF2RB, the common subunit of the IL3, IL5 and CSF2 receptors. Interacts with PAG1; identified in a complex with PAG1 and STAT3. Interacts with ABL1. Interacts with PTPN6/SHP-1. Interacts (via SH3 domain) with SCIMP (via proline-rich region). This interaction facilitates the phosphorylation of SCIMP on 'Tyr-107', which enhances binding of SCIMP to TLR4, and consequently the phosphorylation of TLR4 in response to stimulation by lipopolysaccharide in macrophages (By similarity). Interacts with LPXN (via LD motif 3) and the interaction is induced upon B-cell antigen receptor (BCR) activation. Interacts (via SH3-domain) with ANKRD54 (via ankyrin repeat region) in an activation-independent status of LYN. Forms a multiprotein complex with ANKRD54 and HCLS1. Interacts (via SH2 and SH3 domains) with UNC119; leading to LYN activation. Interacts with CD36. Interacts with LYN (By similarity). Interacts with SKAP1 and FYB1; this interaction promotes the phosphorylation of CLNK (By similarity).

(Microbial infection) Interacts with Epstein-Barr virus LMP2A.

(Microbial infection) Interacts with Herpes virus saimiri tyrosine kinase interacting protein (Tip).

蛋白家族:

The protein kinase domain plays an important role in its localization in the cell membrane.

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

研究领域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Nervous system > Long-term depression.

文献引用

1). Jianbin Sun et al. Anti-allergic actions of a Chinese patent medicine, huoxiangzhengqi oral liquid, in RBL-2H3 cells and in mice. Pharmaceutical Biology 2021 Dec;59(1):672-682. (PubMed: 34078224) [IF=3.8]

Application: WB    Species: Mouse    Sample: RBL-2H3 cells

Figure 5. Inhibitory effects of HXZQ-OL on the phosphorylation of FceRI-induced degranulation signalling cascades (A, B, C) and MAPKs (D) in IgE/Ag-mediated RBL- 2H3 cells. IgE-sensitized RBL-2H3 cells were incubated with HXZQ-OL for 30min, followed by DNP-BSA challenge for 10min. The expression levels of p-Fyn, p-Lyn, p- Syk, p-PLCc1, p-PLCc2, p-PKCd, p-Gabs, p-PI3K, p-Akt, p-ERK1/2, p-p38 and p-JNK1/2 were normalized to total proteins, respectively. The data were expressed as the mean±SD values of three independent experiments. ? p<0.05 and ?? p<0.01.

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