FOLR2 Antibody - #DF9518

Fig. 2 RBA-NPs display targeting capability in vitro. a, b Confocal micrographs showed that DiD-NPs (red) enjoyed significantly improved cell uptake compared with free DiD in LPS + IFN-γ activated RAW264.7 cells. Scale bar = 10 μm. c Flow cytometry analysis showed that the fluorescence intensity of DiD-NPs in LPS + IFN-γ activated RAW264.7 cells was higher than that of free DiD in RAW264.7. d The expression of CD44 and folate receptors increased significantly on the surface of LPS + IFN-γ activated RAW264.7 cells (d). Scale bar = 10 μm. e LPS + IFN-γ activated macrophages were pretreated with HA or FA or both HA and FA to compete for the overexpressed CD44 or folate receptors, and cellular uptake of DiD-NPs was reduced. f Confocal micrographs showed co-localization of DiD-NPs (orange) with CD44 receptor (red) and folate receptor (green). Scale bar = 20 μm. Cell nuclei was stained with DAPI (blue). All results are shown as mean ± SD. *P 

Fig. 3. In vitro cellular uptake of Cy5-labeled NPs. (A) Raw264.7 macrophages induced with LPS (1 μg/mL) for 6 h. Then cellular uptake of Cy5-labeled Dex/Oxi- αCD NPs or Dex/FA-Oxi-αCD NPs was assessed using confocal laser scanning microscopy at various times. Nuclei were counterstained with DAPI (blue). Scale bars, 20 μm. (B) Quantitative analysis of the corresponding fluorescence intensity of intracellular NPs (red) in Raw264.7 cells. (C) The mRNA expression of FRα, FRβ, and FRγ in MH7A cells were analyzed by quantitative real-time PCR. Data were presented by the relative amount of mRNA normalized by GAPDH. (D) Western blot analysis of FRα, FRβ and FRγ expression in Raw264.7 and MH7A cells. α-Tubulin was used as a loading control. (E–F) Cellular uptake (E) and corresponding fluorescence intensity (F) of Cy5-labeled Dex/Oxi-NPs or Dex/FA-Oxi-NPs in MH7A cells at various time points. *p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)