HSD17B12 Antibody - #DF9505

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产品: HSD17B12 抗体
货号: DF9505
描述: Rabbit polyclonal antibody to HSD17B12
应用: WB IHC IF/ICC
反应: Human
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 34 kDa; 34kD(Calculated).
蛋白号: Q53GQ0
RRID: AB_2842701

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(88%), Dog(100%), Chicken(88%)
克隆:
Polyclonal
特异性:
HSD17B12 Antibody detects endogenous levels of total HSD17B12.
RRID:
AB_2842701
引用格式: Affinity Biosciences Cat# DF9505, RRID:AB_2842701.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

12,3-ketoacyl-CoA reductase,short chain; 17 beta HSD 12; 17 beta hydroxysteroid dehydrogenase 12; 17-@beta-HSD XII; 17-@beta-hydroxysteroid dehydrogenase XII; 17-beta-HSD 12; 17-beta-hydroxysteroid dehydrogenase 12; 17beta HSD type 12; 3 ketoacyl CoA reductase; 3-ketoacyl-CoA reductase; DHB12_HUMAN; Estradiol 17 beta dehydrogenase 12; Estradiol 17-beta-dehydrogenase 12; HSD17B12; Hydroxysteroid (17-beta) dehydrogenase 12; KAR; KIK-I; Kik1; SDR12C1; Short chain dehydrogenase/reductase family 12C member 1; Steroid dehydrogenase homolog;

抗原和靶标

免疫原:
Uniprot:

Q53GQ0(DHB12_HUMAN) >>Visit HPA database.

基因/基因ID:
HSD17B12(51144)
表达:
Q53GQ0 DHB12_HUMAN:

Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.

序列:
MESALPAAGFLYWVGAGTVAYLALRISYSLFTALRVWGVGNEAGVGPGLGEWAVVTGSTDGIGKSYAEELAKHGMKVVLISRSKDKLDQVSSEIKEKFKVETRTIAVDFASEDIYDKIKTGLAGLEIGILVNNVGMSYEYPEYFLDVPDLDNVIKKMININILSVCKMTQLVLPGMVERSKGAILNISSGSGMLPVPLLTIYSATKTFVDFFSQCLHEEYRSKGVFVQSVLPYFVATKLAKIRKPTLDKPSPETFVKSAIKTVGLQSRTNGYLIHALMGSIISNLPSWIYLKIVMNMNKSTRAHYLKKTKKN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
88
Chicken
88
Rabbit
88
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q53GQ0 作为底物

Site PTM Type Enzyme
Y12 Phosphorylation
Y66 Phosphorylation
K72 Acetylation
K84 Ubiquitination
K86 Ubiquitination
S91 Phosphorylation
S92 Phosphorylation
K95 Ubiquitination
K97 Ubiquitination
S111 Phosphorylation
Y115 Phosphorylation
K117 Acetylation
K117 Ubiquitination
K119 Ubiquitination
S164 Phosphorylation
T169 Phosphorylation
K181 Ubiquitination
C215 S-Nitrosylation
K223 Ubiquitination
Y233 Phosphorylation
K238 Ubiquitination
K241 Ubiquitination
K244 Ubiquitination
S251 Phosphorylation
K257 Ubiquitination
K261 Ubiquitination
K299 Ubiquitination

研究背景

功能:

Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.

细胞定位:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.

亚基结构:

Interacts with ELOVL1 and LASS2.

蛋白家族:

The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.

Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

研究领域

· Metabolism > Lipid metabolism > Fatty acid elongation.

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

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