产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3102, RRID:AB_2834539.
展开/折叠
C syn protooncogene; Fyn; FYN oncogene related to SRC FGR YES; FYN_HUMAN; OKT3 induced calcium influx regulator; P59 FYN; p59-Fyn; Protein tyrosine kinase fyn; Proto oncogene tyrosine protein kinase fyn; Proto-oncogene c-Fyn; Proto-oncogene Syn; Protooncogene Syn; SLK; Src like kinase; Src yes related novel gene; Src-like kinase; Src/yes related novel; SYN; Tyrosine kinase p59fyn T; Tyrosine kinase p59fyn(T); Tyrosine-protein kinase Fyn;
抗原和靶标
Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.
- P06241 FYN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P06241 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
G2 | Myristoylation | Uniprot | |
T12 | Phosphorylation | P54646 (PRKAA2) | Uniprot |
K13 | Ubiquitination | Uniprot | |
S21 | Phosphorylation | P17612 (PRKACA) | Uniprot |
S25 | Phosphorylation | Uniprot | |
S26 | Phosphorylation | Uniprot | |
Y28 | Phosphorylation | P06241 (FYN) , P09619 (PDGFRB) | Uniprot |
Y30 | Phosphorylation | P06241 (FYN) | Uniprot |
Y39 | Phosphorylation | P06241 (FYN) | Uniprot |
Y91 | Phosphorylation | Uniprot | |
S178 | Phosphorylation | Uniprot | |
T181 | Phosphorylation | Uniprot | |
K182 | Ubiquitination | Uniprot | |
Y185 | Phosphorylation | Uniprot | |
S186 | Phosphorylation | Uniprot | |
S188 | Phosphorylation | Uniprot | |
K196 | Ubiquitination | Uniprot | |
Y213 | Phosphorylation | Uniprot | |
Y214 | Phosphorylation | Uniprot | |
T217 | Phosphorylation | Uniprot | |
S257 | Phosphorylation | Uniprot | |
K259 | Ubiquitination | Uniprot | |
K261 | Ubiquitination | Uniprot | |
K275 | Ubiquitination | Uniprot | |
T289 | Phosphorylation | Uniprot | |
T294 | Phosphorylation | Uniprot | |
K299 | Ubiquitination | Uniprot | |
T300 | Phosphorylation | Uniprot | |
K302 | Ubiquitination | Uniprot | |
S307 | Phosphorylation | Uniprot | |
K319 | Ubiquitination | Uniprot | |
Y339 | Phosphorylation | Uniprot | |
S349 | Phosphorylation | Uniprot | |
K355 | Ubiquitination | Uniprot | |
K405 | Ubiquitination | Uniprot | |
Y420 | Phosphorylation | P06241 (FYN) | Uniprot |
T421 | Phosphorylation | Uniprot | |
K427 | Ubiquitination | Uniprot | |
K431 | Ubiquitination | Uniprot | |
Y440 | Phosphorylation | Uniprot | |
Y483 | Phosphorylation | Uniprot | |
T525 | Phosphorylation | Uniprot | |
T527 | Phosphorylation | Uniprot | |
Y531 | Phosphorylation | P41240 (CSK) | Uniprot |
翻译修饰 - P06241 作为激酶
Substrate | Site | Source |
---|---|---|
A8K4G0 (CD300LB) | Y188 | Uniprot |
O14522-1 (PTPRT) | Y915 | Uniprot |
O14522-1 (PTPRT) | Y1030 | Uniprot |
O14559 (ARHGAP33) | Y406 | Uniprot |
O15117 (FYB1) | Y559 | Uniprot |
O15117 (FYB1) | Y571 | Uniprot |
O15117 (FYB1) | Y595 | Uniprot |
O15117 (FYB1) | Y625 | Uniprot |
O15117 (FYB1) | Y651 | Uniprot |
O15117-2 (FYB1) | Y697 | Uniprot |
O15117 (FYB1) | Y755 | Uniprot |
O15117 (FYB1) | Y757 | Uniprot |
O15117 (FYB1) | Y771 | Uniprot |
O15117 (FYB1) | Y780 | Uniprot |
O43294 (TGFB1I1) | Y60 | Uniprot |
O43490 (PROM1) | Y828 | Uniprot |
O43490 (PROM1) | Y852 | Uniprot |
O43561 (LAT) | Y200 | Uniprot |
O43561 (LAT) | Y220 | Uniprot |
O60500 (NPHS1) | Y1193 | Uniprot |
O60716 (CTNND1) | Y112 | Uniprot |
O75674-1 (TOM1L1) | Y460 | Uniprot |
O75886 (STAM2) | Y291 | Uniprot |
O75886 (STAM2) | Y371 | Uniprot |
O75886 (STAM2) | Y374 | Uniprot |
O75955 (FLOT1) | Y160 | Uniprot |
P00519 (ABL1) | Y70 | Uniprot |
P00519 (ABL1) | Y115 | Uniprot |
P00519 (ABL1) | Y128 | Uniprot |
P00519 (ABL1) | Y139 | Uniprot |
P00519 (ABL1) | Y172 | Uniprot |
P00519 (ABL1) | Y185 | Uniprot |
P00519 (ABL1) | Y215 | Uniprot |
P00519 (ABL1) | Y226 | Uniprot |
P00519 (ABL1) | Y393 | Uniprot |
P05067 (APP) | Y757 | Uniprot |
P06127 (CD5) | Y453 | Uniprot |
P06127 (CD5) | Y487 | Uniprot |
P06241 (FYN) | Y28 | Uniprot |
P06241-1 (FYN) | Y30 | Uniprot |
P06241 (FYN) | Y39 | Uniprot |
P06241-3 (FYN) | Y365 | Uniprot |
P06241-2 (FYN) | Y417 | Uniprot |
P06241 (FYN) | Y420 | Uniprot |
P07766 (CD3E) | Y188 | Uniprot |
P07766 (CD3E) | Y199 | Uniprot |
P10586-2 (PTPRF) | Y1852 | Uniprot |
P10586-1 (PTPRF) | Y1861 | Uniprot |
P10636 (MAPT) | Y18 | Uniprot |
P10747 (CD28) | Y191 | Uniprot |
P11137-3 (MAP2) | Y50 | Uniprot |
P11137 (MAP2) | Y67 | Uniprot |
P11274 (BCR) | Y177 | Uniprot |
P11912-2 (CD79A) | Y161 | Uniprot |
P11912 (CD79A) | Y199 | Uniprot |
P12318-2 (FCGR2A) | Y280 | Uniprot |
P12318 (FCGR2A) | Y281 | Uniprot |
P12318-2 (FCGR2A) | Y287 | Uniprot |
P12318 (FCGR2A) | Y288 | Uniprot |
P12318-2 (FCGR2A) | Y303 | Uniprot |
P12318 (FCGR2A) | Y304 | Uniprot |
P14923 (JUP) | Y133 | Uniprot |
P14923 (JUP) | Y550 | Uniprot |
P16284 (PECAM1) | Y690 | Uniprot |
P16284 (PECAM1) | Y713 | Uniprot |
P16410-1 (CTLA4) | Y201 | Uniprot |
P16410 (CTLA4) | Y218 | Uniprot |
P16885 (PLCG2) | Y743 | Uniprot |
P16885 (PLCG2) | Y753 | Uniprot |
P16885 (PLCG2) | Y759 | Uniprot |
P20916-1 (MAG) | Y620 | Uniprot |
P20963 (CD247) | Y111 | Uniprot |
P20963 (CD247) | Y123 | Uniprot |
P22681 (CBL) | Y700 | Uniprot |
P22681 (CBL) | Y731 | Uniprot |
P22681 (CBL) | Y774 | Uniprot |
P24666-2 (ACP1) | Y132 | Uniprot |
P24666-1 (ACP1) | Y133 | Uniprot |
P29353-7 (SHC1) | Y318 | Uniprot |
P29353 (SHC1) | Y349 | Uniprot |
P29353 (SHC1) | Y350 | Uniprot |
P29353 (SHC1) | Y427 | Uniprot |
P30419 (NMT1) | Y180 | Uniprot |
P31994 (FCGR2B) | Y292 | Uniprot |
P35222 (CTNNB1) | Y142 | Uniprot |
P35354 (PTGS2) | Y446 | Uniprot |
P35568 (IRS1) | Y896 | Uniprot |
P35568 (IRS1) | Y1179 | Uniprot |
P35612 (ADD2) | Y489 | Uniprot |
P35637 (FUS) | Y526 | Uniprot |
P37840-1 (SNCA) | Y125 | Uniprot |
P40259-2 (CD79B) | Y92 | Uniprot |
P40259-2 (CD79B) | Y103 | Uniprot |
P40259 (CD79B) | Y196 | Uniprot |
P40259-1 (CD79B) | Y207 | Uniprot |
P42681 (TXK) | Y420 | Uniprot |
P42768 (WAS) | Y291 | Uniprot |
P43146 (DCC) | Y1420 | Uniprot |
P49023-2 (PXN) | Y118 | Uniprot |
P51812 (RPS6KA3) | Y470 | Uniprot |
P51812 (RPS6KA3) | Y483 | Uniprot |
P51812 (RPS6KA3) | Y488 | Uniprot |
P51812 (RPS6KA3) | Y529 | Uniprot |
P51812 (RPS6KA3) | Y580 | Uniprot |
P51812 (RPS6KA3) | Y644 | Uniprot |
P51812 (RPS6KA3) | Y707 | Uniprot |
P51911-1 (CNN1) | Y182 | Uniprot |
P51911-1 (CNN1) | Y261 | Uniprot |
P52757 (CHN2) | Y21 | Uniprot |
P78352 (DLG4) | Y523 | Uniprot |
P84243 (H3F3B) | S11 | Uniprot |
Q00535-1 (CDK5) | Y15 | Uniprot |
Q01628 (IFITM3) | Y20 | Uniprot |
Q03135 (CAV1) | Y14 | Uniprot |
Q05397 (PTK2) | Y576 | Uniprot |
Q05655 (PRKCD) | Y313 | Uniprot |
Q05655 (PRKCD) | Y334 | Uniprot |
Q06124 (PTPN11) | Y546 | Uniprot |
Q07912-1 (TNK2) | Y284 | Uniprot |
Q12879 (GRIN2A) | Y943 | Uniprot |
Q12879 (GRIN2A) | Y1105 | Uniprot |
Q12879 (GRIN2A) | Y1118 | Uniprot |
Q12879 (GRIN2A) | Y1187 | Uniprot |
Q12879 (GRIN2A) | Y1246 | Uniprot |
Q12879 (GRIN2A) | Y1267 | Uniprot |
Q12879 (GRIN2A) | Y1325 | Uniprot |
Q12913 (PTPRJ) | Y1311 | Uniprot |
Q12913 (PTPRJ) | Y1320 | Uniprot |
Q13224 (GRIN2B) | Y932 | Uniprot |
Q13224 (GRIN2B) | Y1039 | Uniprot |
Q13224 (GRIN2B) | Y1070 | Uniprot |
Q13224 (GRIN2B) | Y1109 | Uniprot |
Q13224 (GRIN2B) | Y1252 | Uniprot |
Q13224 (GRIN2B) | Y1336 | Uniprot |
Q13224 (GRIN2B) | Y1474 | Uniprot |
Q13291 (SLAMF1) | Y281 | Uniprot |
Q13291-1 (SLAMF1) | Y307 | Uniprot |
Q13291 (SLAMF1) | Y327 | Uniprot |
Q13322-3 (GRB10) | Y9 | Uniprot |
Q13322 (GRB10) | Y67 | Uniprot |
Q14194 (CRMP1) | Y504 | Uniprot |
Q14254 (FLOT2) | Y163 | Uniprot |
Q14524 (SCN5A) | Y1494 | Uniprot |
Q14524 (SCN5A) | Y1495 | Uniprot |
Q14643 (ITPR1) | Y353 | Uniprot |
Q15417-1 (CNN3) | Y261 | Uniprot |
Q15762 (CD226) | Y322 | Uniprot |
Q15831 (STK11) | Y261 | Uniprot |
Q15831 (STK11) | Y362 | Uniprot |
Q16236 (NFE2L2) | Y576 | Uniprot |
Q16539 (MAPK14) | Y323 | Uniprot |
Q16555 (DPYSL2) | Y32 | Uniprot |
Q6ZMQ8 (AATK) | Y73 | Uniprot |
Q6ZMQ8 (AATK) | Y93 | Uniprot |
Q86WV1 (SKAP1) | Y219 | Uniprot |
Q86WV1 (SKAP1) | Y232 | Uniprot |
Q86WV1 (SKAP1) | Y295 | Uniprot |
Q86WV1 (SKAP1) | Y298 | Uniprot |
Q8TDQ1 (CD300LF) | Y236 | Uniprot |
Q8TDQ1 (CD300LF) | Y263 | Uniprot |
Q96J02 (ITCH) | Y420 | Uniprot |
Q96J84 (KIRREL1) | Y572 | Uniprot |
Q96J84 (KIRREL1) | Y605 | Uniprot |
Q96J84 (KIRREL1) | Y622 | Uniprot |
Q96LC7 (SIGLEC10) | Y667 | Uniprot |
Q99490-2 (AGAP2) | Y682 | Uniprot |
Q99490-2 (AGAP2) | Y774 | Uniprot |
Q99490 (AGAP2) | Y1038 | Uniprot |
Q99490 (AGAP2) | Y1130 | Uniprot |
Q9H204 (MED28) | Y64 | Uniprot |
Q9NQC3 (RTN4) | Y694 | Uniprot |
Q9NRY4 (ARHGAP35) | Y1087 | Uniprot |
Q9NRY4 (ARHGAP35) | Y1105 | Uniprot |
Q9NZA1 (CLIC5) | Y33 | Uniprot |
研究背景
Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling (By similarity).
Autophosphorylated at Tyr-420 (By similarity). Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state. PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-12 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling. Phosphorylated at tyrosine residues, which can be enhanced by NTN1 (By similarity).
Palmitoylation at Cys-3 and Cys-6 regulates subcellular location.
Cytoplasm. Nucleus. Cell membrane.
Note: Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking.
Isoform 1 is highly expressed in the brain. Isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes.
Interacts (via its SH3 domain) with PIK3R1 and PRMT8. Interacts with FYB1, PAG1, and SH2D1A. Interacts with CD79A (tyrosine-phosphorylated form); the interaction increases FYN activity. Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated) (By similarity). Interacts with TOM1L1 (phosphorylated form). Interacts with KDR (tyrosine phosphorylated). Interacts (via SH3 domain) with KLHL2 (via N-terminus) (By similarity). Interacts with SH2D1A and SLAMF1. Interacts with ITCH; the interaction phosphorylates ITCH and negatively regulates its activity. Interacts with FASLG. Interacts with RUNX3. Interacts with KIT. Interacts with EPHA8; possible downstream effector of EPHA8 in regulation of cell adhesion. Interacts with PTK2/FAK1; this interaction leads to PTK2/FAK1 phosphorylation and activation. Interacts with CAV1; this interaction couples integrins to the Ras-ERK pathway. Interacts with UNC119. Interacts (via SH2 domain) with PTPRH (phosphorylated form) (By similarity). Interacts with PTPRO (phosphorylated form) (By similarity). Interacts with PTPRB (phosphorylated form) (By similarity). Interacts with FYB2. Interacts with DSCAM (By similarity). Interacts with SKAP1 and FYB1; this interaction promotes the phosphorylation of CLNK (By similarity).
(Microbial infection) Interacts (via its SH3 domain) with hepatitis E virus/HEV protein ORF3.
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Human Diseases > Neurodegenerative diseases > Prion diseases.
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Nervous system > Cholinergic synapse.
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