Phospho-Lck (Tyr393)[Tyr394] Antibody - #AF3101

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产品: 磷酸化 Lck (Tyr393)[Tyr394] 抗体
货号: AF3101
描述: Rabbit polyclonal antibody to Phospho-Lck (Tyr393)[Tyr394]
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P06239
RRID: AB_2834538

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-Lck (Tyr393) Antibody detects endogenous levels of Lck only when phosphorylated at Tyr394, which site historically referenced as Tyr393.
RRID:
AB_2834538
引用格式: Affinity Biosciences Cat# AF3101, RRID:AB_2834538.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

IMD22; LCK; Lck p56; LCK proto-oncogene, Src family tyrosine kinase; LCK_HUMAN; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell specific protein tyrosine kinase; Lymphocyte cell-specific protein-tyrosine kinase; Lymphocyte specific protein tyrosine kinase; Membrane associated protein tyrosine kinase; Oncogene lck; P56 LCK; p56(LSTRA) protein tyrosine kinase; p56-LCK; p56lck; pp58 lck; pp58lck; Protein YT16; Proto oncogene tyrosine protein kinase LCK; Proto-oncogene Lck; Protooncogene tyrosine protein kinase LCK; T cell specific protein tyrosine kinase; T cell-specific protein-tyrosine kinase; T lymphocyte specific protein tyrosine kinase p56lck; Tyrosine-protein kinase Lck; YT 16; YT16;

抗原和靶标

免疫原:

A synthesized peptide derived from human Lck around the phosphorylation site of Tyr393.

基因/基因ID:
LCK(3932)
描述:
Tyrosine kinase that plays an essential role for the selection and maturation of developing t-cell in the thymus and in mature t-cell function.

研究领域

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > Th17 cell differentiation.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

文献引用

1). Regulation of T Cell Activities in Rheumatoid Arthritis by the Novel Fusion Protein IgD-Fc-Ig. Frontiers in Immunology, 2020 (PubMed: 32499775) [IF=5.7]

Application: WB    Species: mouse    Sample: spleen

FIGURE 9 | Effects of IgD-Fc-Ig (DG) on the protein expression of Lck, p-Lck, ZAP70 and p-ZAP70. (A–C) Western blot analysis of p-Lck and p-ZAP70 expression in PBMCs of RA patients, PBMCs were treated with IgD (3µg/mL) and IgD-Fc-Ig (1, 3, 10µg/mL) for 48h and lysed. (D–F) Western blot analysis of Lck, p-Lck, ZAP70 and p-ZAP70 expression in CIA mice. Mice spleens from each group were isolated and homogenized in lysis buffer.
2). CCL5 promotes LFA-1 expression in Th17 cells and induces LCK and ZAP70 activation in a mouse model of Parkinson's disease. Frontiers in aging neuroscience, 2023 (PubMed: 38020765) [IF=4.1]

Application: WB    Species: Mouse    Sample:

Figure 4. CCL5 promoted Th17 cell differentiation and LFA-1 expression by activating LCK and ZAP70. (A) Flow cytometry and FlowJo software were used to analyze peripheral PBMCs from mice. The proportions of IL-17A+, IL-17A+ and LFA-1+, IL-17A+ and pLCK+, IL-17A+ and pZAP70+ cells among CD4+ cell populations were calculated n = 5 per group. (B) Naive CD4+ T cells that were cultured in vitro were induced to differentiate into Th17 cells, which were treated with CCL5 or PBS. After 3 days, LFA-1, pLCK, pZAP70, total LCK, total ZAP70 and Rorγt levels were measured by Western blot analysis and quantified using ImageJ n = 3 per group. Data are presented as the means ± SEMs. *p 

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