CATSPER4 Antibody - #DF9351

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产品: CATSPER4 抗体
货号: DF9351
描述: Rabbit polyclonal antibody to CATSPER4
应用: WB IHC IF/ICC
文献验证: WB
反应: Human
预测: Pig, Horse, Sheep, Rabbit
分子量: 50~60kDa; 54kD(Calculated).
蛋白号: Q7RTX7
RRID: AB_2842547

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(88%), Horse(%), Sheep(%), Rabbit(%)
克隆:
Polyclonal
特异性:
CATSPER4 Antibody detects endogenous levels of total CATSPER4.
RRID:
AB_2842547
引用格式: Affinity Biosciences Cat# DF9351, RRID:AB_2842547.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cation channel sperm associated 4; Cation channel sperm associated protein 4; CatSper4; Channel sperm associated 4; Ion channel CatSper4; MGC149223;

抗原和靶标

免疫原:
Uniprot:

Q7RTX7(CTSR4_HUMAN) >>Visit HPA database.

基因/基因ID:
CATSPER4(378807)
表达:
Q7RTX7 CTSR4_HUMAN:

Testis-specific.

序列:
MRDNEKAWWQQWTSHTGLEGWGGTQEDRMGFGGAVAALRGRPSPLQSTIHESYGRPEEQVLINRQEITNKADAWDMQEFITHMYIKQLLRHPAFQLLLALLLVINAITIALRTNSYLDQKHYELFSTIDDIVLTILLCEVLLGWLNGFWIFWKDGWNILNFIIVFILLLRFFINEINIPSINYTLRALRLVHVCMAVEPLARIIRVILQSVPDMANIMVLILFFMLVFSVFGVTLFGAFVPKHFQNIQVALYTLFICITQDGWVDIYSDFQTEKREYAMEIGGAIYFTIFITIGAFIGINLFVIVVTTNLEQMMKAGEQGQQQRITFSETGAEEEEENDQLPLVHCVVARSEKSGLLQEPLAGGPLSNLSENTCDNFCLVLEAIQENLRQYKEIRDELNMIVEEVRAIRFNQEQESEVLNRRSSTSGSLETTSSKDIRQMSQQQDLLSALVSMEKVHDSSSQILLKKHKSSH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
88
Horse
88
Sheep
88
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte.

细胞定位:

Cell projection>Cilium>Flagellum membrane>Multi-pass membrane protein.
Note: Specifically located in the principal piece of sperm tail.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Testis-specific.

亚基结构:

Heterotetramer; possibly composed of CATSPER1, CATSPER2, CATSPER3 and CATSPER4 (Potential). Component of the CatSper complex. Interacts with CATSPER1 (By similarity).

蛋白家族:

Belongs to the cation channel sperm-associated (TC 1.A.1.19) family.

文献引用

1). The Olfactory Receptor Olfr25 Mediates Sperm Dysfunction Induced by Low-Dose Bisphenol A through the CatSper-Ca2+ Signaling Pathway. Toxics, 2024 (PubMed: 38922122) [IF=4.6]

Application: WB    Species: Mouse    Sample: GC-2 cells

Figure 4 The effect of Olfr25 knockdown on calcium ion levels and the expression of CatSper channel genes in GC-2 cells. (A) RT-qPCR was used to measure the mRNA expression levels of Olfr25 (n = 3). (B) Intracellular calcium ion concentration was detected after Olfr25 knockdown (n = 3). (C) The mRNA expression levels of Catsper1–4 in GC-2 cells after Olfr25 knockdown were detected using qRT-PCR (n = 3). (D) The protein expression levels of CatSper1–4 in GC-2 cells after Olfr25 knockdown were detected using Western blot analyses. (E) Quantitative analysis of the indicated proteins using Image J software. All data are presented as the mean ± SD. ns, not significant. ** p < 0.01.

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