CATSPER1 Antibody - #DF9349

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产品: CATSPER1 抗体
货号: DF9349
描述: Rabbit polyclonal antibody to CATSPER1
应用: WB IHC IF/ICC
文献验证: WB, IF/ICC
反应: Human, Mouse
预测: Sheep, Rabbit
分子量: 90 kDa; 90kD(Calculated).
蛋白号: Q8NEC5
RRID: AB_2842545

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
预测:
Sheep(83%), Rabbit(%)
克隆:
Polyclonal
特异性:
CATSPER1 Antibody detects endogenous levels of total CATSPER1.
RRID:
AB_2842545
引用格式: Affinity Biosciences Cat# DF9349, RRID:AB_2842545.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cation channel sperm associated 1; Cation channel sperm associated protein 1; Cation channel sperm-associated protein 1; CATSPER 1; CATSPER; CatSper1; CTSR1_HUMAN; hCatSper; MGC33335; MGC33368; Sperm associated cation channel 1; Sperm ion channel;

抗原和靶标

免疫原:
Uniprot:

Q8NEC5(CTSR1_HUMAN) >>Visit HPA database.

基因/基因ID:
CATSPER1(117144)
表达:
Q8NEC5 CTSR1_HUMAN:

Testis-specific.

序列:
MDQNSVPEKAQNEADTNNADRFFRSHSSPPHHRPGHSRALHHYELHHHGVPHQRGESHHPPEFQDFHDQALSSHVHQSHHHSEARNHGRAHGPTGFGLAPSQGAVPSHRSYGEDYHDELQRDGRRHHDGSQYGGFHQQSDSHYHRGSHHGRPQYLGENLSHYSSGVPHHGEASHHGGSYLPHGPNPYSESFHHSEASHLSGLQHDESQHHQVPHRGWPHHHQVHHHGRSRHHEAHQHGKSPHHGETISPHSSVGSYQRGISDYHSEYHQGDHHPSEYHHGDHPHHTQHHYHQTHRHRDYHQHQDHHGAYHSSYLHGDYVQSTSQLSIPHTSRSLIHDAPGPAASRTGVFPYHVAHPRGSAHSMTRSSSTIRSRVTQMSKKVHTQDISTKHSEDWGKEEGQFQKRKTGRLQRTRKKGHSTNLFQWLWEKLTFLIQGFREMIRNLTQSLAFETFIFFVVCLNTVMLVAQTFAEVEIRGEWYFMALDSIFFCIYVVEALLKIIALGLSYFFDFWNNLDFFIMAMAVLDFLLMQTHSFAIYHQSLFRILKVFKSLRALRAIRVLRRLSFLTSVQEVTGTLGQSLPSIAAILILMFTCLFLFSAVLRALFRKSDPKRFQNIFTTIFTLFTLLTLDDWSLIYMDSRAQGAWYIIPILVIYIIIQYFIFLNLVITVLVDSFQTALFKGLEKAKQERAARIQEKLLEDSLTELRAAEPKEVASEGTMLKRLIEKKFGTMTEKQQELLFHYLQLVASVEQEQQKFRSQAAVIDEIVDTTFEAGEEDFRN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Sheep
83
Rabbit
80
Pig
0
Horse
0
Bovine
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Voltage-gated calcium channel that plays a central role in calcium-dependent physiological responses essential for successful fertilization, such as sperm hyperactivation, acrosome reaction and chemotaxis towards the oocyte.

细胞定位:

Cell projection>Cilium>Flagellum membrane>Multi-pass membrane protein.
Note: Specifically located in the principal piece of sperm tail.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Testis-specific.

亚基结构:

Heterotetramer; possibly composed of CATSPER1, CATSPER2, CATSPER3 and CATSPER4 (Potential). Component of the CatSper complex. Interacts with CATSPER3 and CATSPER4 (By similarity). Interacts with Ca(v)3.3/CACNA1I, leading to suppress T-type calcium channel activity.

蛋白家族:

Belongs to the cation channel sperm-associated (TC 1.A.1.19) family.

文献引用

1). Homozygous mutation in SLO3 leads to severe asthenoteratozoospermia due to acrosome hypoplasia and mitochondrial sheath malformations. Reproductive Biology and Endocrinology, 2022 (PubMed: 34980136) [IF=4.4]

Application: WB    Species: Human    Sample: sperm cells

Fig. 6 Membrane potential and expression patterns of LRRC52 and CatSper1 examined in the spermatozoa from control and SLO3-mutant subjects. A Membrane potential measurements of wild-type and SLO3 mutant sperm in capacitated conditions. The capacitation-associated hyperpolarization of human sperm is inhibited in SLO3 mutant spermatozoa, Data are presented as the mean ± SEM. ***P < 0.001; Student’s t-test. B Immunoblotting of sperm lysates from controls and the SLO3-mutated individual using the anti-LRRC52 and CatSper1 antibodies. Na+/K+-ATPase α1 was used as loading control. The expression of the LRRC52 protein is decreased in mutant sperm, whereas the level of the CatSper1 protein is comparable to that of control. C Representative images of spermatozoa from fertile controls and the SLO3 mutant patient stained with the anti-LRRC52 antibody, anti-CatSper1 antibody, anti-Ac-tubulin antibody, and Hoechst. Two independent experiments were performed and at least 150 sperm we re-examined for each time per individual. Scale bars, 20 μm

Application: IF/ICC    Species: Human    Sample: sperm cells

Fig. 6 Membrane potential and expression patterns of LRRC52 and CatSper1 examined in the spermatozoa from control and SLO3-mutant subjects. A Membrane potential measurements of wild-type and SLO3 mutant sperm in capacitated conditions. The capacitation-associated hyperpolarization of human sperm is inhibited in SLO3 mutant spermatozoa, Data are presented as the mean ± SEM. ***P < 0.001; Student’s t-test. B Immunoblotting of sperm lysates from controls and the SLO3-mutated individual using the anti-LRRC52 and CatSper1 antibodies. Na+/K+-ATPase α1 was used as loading control. The expression of the LRRC52 protein is decreased in mutant sperm, whereas the level of the CatSper1 protein is comparable to that of control. C Representative images of spermatozoa from fertile controls and the SLO3 mutant patient stained with the anti-LRRC52 antibody, anti-CatSper1 antibody, anti-Ac-tubulin antibody, and Hoechst. Two independent experiments were performed and at least 150 sperm we re-examined for each time per individual. Scale bars, 20 μm

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