CEACAM7 Antibody - #DF9346

Figure 6. Validation of the ROS-CPXM2-EMT axis in oxidative stress cell models. A: Representative images of Western blotting (WB) analysis of 4-hydroxynonenal (4-HNE) and CPXM2 in ARPE-19 cells treated with or without H2O2 (200 μmol/L), and the relative quantification of 4-HNE and CPXM2. B: Representative immunofluorescence images and mean fluorescence intensity analysis of CPXM2 in ARPE-19 cells treated with H2O2 (200 μmol/L). Scale bar: 50 μm. C: WB analysis of fibronectin 1 (FN1) and SNAIL in ARPE-19 cells treated with H2O2 (200 μmol/L), and the relative quantification of FN1 and SNAIL expression. D: Representative immunofluorescence images and mean fluorescence intensity analysis of ZO-1 and bestrophin-1 (BEST1) in ARPE-19 cells treated with H2O2 (200 μmol/L). Scale bar: 50 μm. E: Quantitative reverse transcription PCR analyses were performed to detect the expression levels of CPXM2 mRNA in ARPE-19 cells transfected with CPXM2 siRNA (siCPXM2). F: Images of WB analysis of ARPE-19 cells transfected with negative siRNA (siNC) or siCPXM2 and stimulated with PBS or H2O2 (200 μmol/L), and the relative quantification analysis of FN1, CPXM2 and αSMA expression. The data are presented as means with standard errors of the mean from three independent experiments. Student's t-test was applied for comparisons between two groups. ANOVA and Tukey's Honestly-Significant Difference post hoc test were applied for comparisons between the three groups

Figure 6. Validation of the ROS-CPXM2-EMT axis in oxidative stress cell models. A: Representative images of Western blotting (WB) analysis of 4-hydroxynonenal (4-HNE) and CPXM2 in ARPE-19 cells treated with or without H2O2 (200 μmol/L), and the relative quantification of 4-HNE and CPXM2. B: Representative immunofluorescence images and mean fluorescence intensity analysis of CPXM2 in ARPE-19 cells treated with H2O2 (200 μmol/L). Scale bar: 50 μm. C: WB analysis of fibronectin 1 (FN1) and SNAIL in ARPE-19 cells treated with H2O2 (200 μmol/L), and the relative quantification of FN1 and SNAIL expression. D: Representative immunofluorescence images and mean fluorescence intensity analysis of ZO-1 and bestrophin-1 (BEST1) in ARPE-19 cells treated with H2O2 (200 μmol/L). Scale bar: 50 μm. E: Quantitative reverse transcription PCR analyses were performed to detect the expression levels of CPXM2 mRNA in ARPE-19 cells transfected with CPXM2 siRNA (siCPXM2). F: Images of WB analysis of ARPE-19 cells transfected with negative siRNA (siNC) or siCPXM2 and stimulated with PBS or H2O2 (200 μmol/L), and the relative quantification analysis of FN1, CPXM2 and αSMA expression. The data are presented as means with standard errors of the mean from three independent experiments. Student's t-test was applied for comparisons between two groups. ANOVA and Tukey's Honestly-Significant Difference post hoc test were applied for comparisons between the three groups