Phospho-Cytokeratin 8 (Ser432) Antibody - #AF3097

(9)  
产品: 磷酸化 Cytokeratin 8 (Ser432) 抗体
货号: AF3097
描述: Rabbit polyclonal antibody to Phospho-Cytokeratin 8 (Ser432)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
分子量: 53kDa; 54kD(Calculated).
蛋白号: P05787
RRID: AB_2834534

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1300 现货
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Phospho-Cytokeratin 8 (Ser432) Antibody detects endogenous levels of Cytokeratin 8 only when phosphorylated at Serine 432.
RRID:
AB_2834534
引用格式: Affinity Biosciences Cat# AF3097, RRID:AB_2834534.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CARD2; CK 8; CK-8; CK8; CYK8; CYKER; Cytokeratin endo A; Cytokeratin-8; DreK8; EndoA; K2C8; K2C8_HUMAN; K8; Keratin 8; Keratin type II cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; KO; Krt 2.8; KRT8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8;

抗原和靶标

免疫原:
Uniprot:

P05787(K2C8_HUMAN) >>Visit HPA database.

基因/基因ID:
KRT8(3856)
表达:
P05787 K2C8_HUMAN:

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

描述:
K8 a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins.
序列:
MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK

翻译修饰 - P05787 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
T6 Phosphorylation Q96GD4 (AURKB)
K8 Acetylation
K8 Methylation
K8 Ubiquitination
S9 Phosphorylation P17612 (PRKACA)
Y10 Phosphorylation
K11 Acetylation
K11 Methylation
K11 Sumoylation
K11 Ubiquitination
S13 O-Glycosylation
S13 Phosphorylation P17612 (PRKACA)
T14 Phosphorylation
S15 O-Glycosylation
S15 Phosphorylation
R18 Methylation
S21 Phosphorylation
S22 Phosphorylation
R23 Methylation
S24 Phosphorylation P17612 (PRKACA)
Y25 Phosphorylation
T26 Phosphorylation
S27 Phosphorylation
S31 Phosphorylation
R32 Methylation
S34 Phosphorylation P17612 (PRKACA)
S35 Phosphorylation
S36 Phosphorylation
S37 Phosphorylation P17612 (PRKACA)
S39 Phosphorylation
R40 Methylation
S43 Phosphorylation P17612 (PRKACA)
S44 Phosphorylation
R47 Methylation
Y54 Phosphorylation
S58 Phosphorylation
T64 Phosphorylation
T67 Phosphorylation
S71 Phosphorylation
S74 Phosphorylation P36507 (MAP2K2) , O15264 (MAPK13) , Q05655 (PRKCD) , P53778 (MAPK12) , Q16539 (MAPK14) , Q15759 (MAPK11) , Q02750 (MAP2K1) , P45983 (MAPK8)
T89 Phosphorylation
K92 Ubiquitination
K96 Ubiquitination
T97 Phosphorylation
K101 Acetylation
K101 Methylation
K101 Sumoylation
K101 Ubiquitination
S104 Phosphorylation
K108 Acetylation
K108 Ubiquitination
K117 Acetylation
K117 Ubiquitination
K122 Acetylation
K122 Methylation
K122 Ubiquitination
S124 Phosphorylation
K130 Acetylation
K130 Sumoylation
K130 Ubiquitination
T131 Phosphorylation
S134 Phosphorylation
S142 Phosphorylation
Y143 Phosphorylation
K158 Acetylation
K158 Ubiquitination
K160 Ubiquitination
K176 Ubiquitination
K178 Methylation
K178 Ubiquitination
Y179 Phosphorylation
K185 Methylation
K185 Ubiquitination
T187 Phosphorylation
K197 Sumoylation
K197 Ubiquitination
K198 Ubiquitination
Y204 Phosphorylation
K207 Acetylation
K207 Ubiquitination
S212 Phosphorylation
T218 Phosphorylation
Y228 Phosphorylation
S240 Phosphorylation
T242 Phosphorylation
S243 Phosphorylation
S247 Phosphorylation
S251 Phosphorylation
S253 Phosphorylation
S258 Phosphorylation
K264 Ubiquitination
Y267 Phosphorylation
S274 Phosphorylation
S280 Phosphorylation
Y282 Phosphorylation
K285 Acetylation
K285 Methylation
K285 Sumoylation
K285 Ubiquitination
Y286 Phosphorylation
S291 Phosphorylation
K295 Acetylation
K295 Ubiquitination
T303 Phosphorylation
K304 Sumoylation
K304 Ubiquitination
T305 Phosphorylation
S308 Phosphorylation
S315 Phosphorylation
K325 Acetylation
K325 Sumoylation
K325 Ubiquitination
S330 Phosphorylation
K347 Acetylation
K347 Ubiquitination
K352 Acetylation
K352 Ubiquitination
S354 Phosphorylation
K364 Sumoylation
Y374 Phosphorylation
K393 Acetylation
K393 Sumoylation
K393 Ubiquitination
S400 Phosphorylation
S404 Phosphorylation
S410 Phosphorylation
T413 Phosphorylation
T415 Phosphorylation
T416 Phosphorylation
S417 Phosphorylation P17612 (PRKACA)
Y419 Phosphorylation
S424 Phosphorylation
S425 Phosphorylation
Y427 Phosphorylation
T431 Phosphorylation P28482 (MAPK1)
S432 Phosphorylation P27361 (MAPK3) , P45983 (MAPK8) , P06493 (CDK1)
S436 Phosphorylation
Y437 Phosphorylation
S438 Phosphorylation
S441 Phosphorylation
S442 Phosphorylation
S445 Phosphorylation
S449 Phosphorylation
S450 Phosphorylation
S451 Phosphorylation
S453 Phosphorylation
T455 Phosphorylation
S456 Phosphorylation
S457 Phosphorylation
S458 Phosphorylation
K464 Acetylation
K465 Acetylation
K465 Ubiquitination
T468 Phosphorylation
K472 Acetylation
K472 Sumoylation
K472 Ubiquitination
S475 O-Glycosylation
S475 Phosphorylation
S477 Phosphorylation
S478 Phosphorylation
K483 Acetylation

研究背景

功能:

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

翻译修饰:

Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.

O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

细胞定位:

Cytoplasm. Nucleus>Nucleoplasm. Nucleus matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

亚基结构:

Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1. Interacts with GPER1. Interacts with EPPK1 (By similarity).

(Microbial infection) Interacts with hepatitis C virus/HCV core protein.

蛋白家族:

Belongs to the intermediate filament family.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.