产品: Cytokeratin 5 抗体
货号: AF0194
描述: Rabbit polyclonal antibody to Cytokeratin 5
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Rabbit
分子量: 62kDa; 62kD(Calculated).
蛋白号: P13647
RRID: AB_2833387

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Rabbit(100%)
克隆:
Polyclonal
特异性:
Cytokeratin 5 Antibody detects endogenous levels of total Cytokeratin 5.
RRID:
AB_2833387
引用格式: Affinity Biosciences Cat# AF0194, RRID:AB_2833387.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

58 kDa cytokeratin; CK-5; CK5; Cytokeratin-5; Cytokeratin5; DDD; DDD1; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K2C5_HUMAN; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin; keratin complex 2, basic, gene 5; keratin, type II cytoskeletal 5; Keratin-5; Keratin5; KRT 5; Krt5; KRT5A; type II cytoskeletal 5; Type-II keratin Kb5;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
K5 Belongs to the intermediate filament family. Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP.
序列:
MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGYGSRSLYNLGGSKRISISTSGGSFRNRFGAGAGGGYGFGGGAGSGFGFGGGAGGGFGLGGGAGFGGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPSIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWTLLQEQGTKTVRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRNMQDLVEDFKNKYEDEINKRTTAENEFVMLKKDVDAAYMNKVELEAKVDALMDEINFMKMFFDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIANRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIADAEQRGELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGSSGSYYSSSSGGVGLGGGLSVGGSGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Rabbit
100
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P13647 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
S5 Phosphorylation
S8 Phosphorylation
S11 Phosphorylation
S14 Phosphorylation
S16 Phosphorylation
S18 Phosphorylation
T19 Phosphorylation
S21 Phosphorylation
T24 Phosphorylation
S26 Phosphorylation
S28 Phosphorylation
S31 Phosphorylation
S36 Phosphorylation
S38 Phosphorylation
S50 Phosphorylation
Y60 Phosphorylation
S62 Phosphorylation
S64 Phosphorylation
Y66 Phosphorylation
S71 Phosphorylation
K72 Acetylation
K72 Methylation
S75 Phosphorylation
S77 Phosphorylation
T78 Phosphorylation
S82 Phosphorylation
T151 Phosphorylation
K173 Ubiquitination
T174 Phosphorylation
K178 Acetylation
K178 Methylation
K178 Sumoylation
K178 Ubiquitination
S181 Phosphorylation
K185 Acetylation
K185 Ubiquitination
K199 Methylation
Y222 Phosphorylation
S242 Phosphorylation
K257 Methylation
K257 Ubiquitination
Y258 Phosphorylation
K264 Methylation
K264 Ubiquitination
Y283 Phosphorylation
S337 Phosphorylation
Y346 Phosphorylation
S353 Phosphorylation
T355 Phosphorylation
S359 Phosphorylation
Y361 Phosphorylation
T363 Phosphorylation
Y365 Phosphorylation
K426 Ubiquitination
K443 Sumoylation
Y453 Phosphorylation
R480 Methylation
S567 Phosphorylation
S571 Phosphorylation
S574 Phosphorylation
S579 Phosphorylation
S587 Phosphorylation
S590 Phosphorylation

研究背景

亚基结构:

Heterodimer of a type I and a type II keratin. Heterodimer with type I keratin KRT25 leading to the formation of keratin intermediate filament (KIF) network. Heterodimer with KRT14. Interacts with TCHP. Interacts with EPPK1 (By similarity).

蛋白家族:

Belongs to the intermediate filament family.

文献引用

1). Jiang L et al. Decreased HMGB1 expression contributed to cutaneous toxicity caused by lapatinib. Biochemical Pharmacology 2022 May 23; (PubMed: 35617997) [IF=5.8]

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