产品: CaMKII delta 抗体
货号: DF9300
描述: Rabbit polyclonal antibody to CaMKII delta
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Sheep, Rabbit, Dog
分子量: 56 kDa; 56kD(Calculated).
蛋白号: Q13557
RRID: AB_2842496

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Sheep(100%), Rabbit(91%), Dog(91%)
克隆:
Polyclonal
特异性:
CAMK2D Antibody detects endogenous levels of total CAMK2D.
RRID:
AB_2842496
引用格式: Affinity Biosciences Cat# DF9300, RRID:AB_2842496.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Calcium / calmodulin dependent protein kinase 2 delta; Calcium / calmodulin dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase type II delta chain; Calcium/calmodulin-dependent protein kinase type II subunit delta; CAM kinase 2 delta; CAM kinase II delta; CaM kinase II delta subunit; CaM kinase II subunit delta; CaM-kinase II delta chain; CAMK 2d; CaMK-II delta subunit; CaMK-II subunit delta; CAMK2D; CAMKD; CAMKI; KCC2D_HUMAN; RATCAMKI;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q13557 KCC2D_HUMAN:

Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.

序列:
MASTTTCTRFTDEYQLFEELGKGAFSVVRRCMKIPTGQEYAAKIINTKKLSARDHQKLEREARICRLLKHPNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILESVNHCHLNGIVHRDLKPENLLLASKSKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKDPYGKPVDMWACGVILYILLVGYPPFWDEDQHRLYQQIKAGAYDFPSPEWDTVTPEAKDLINKMLTINPAKRITASEALKHPWICQRSTVASMMHRQETVDCLKKFNARRKLKGAILTTMLATRNFSAAKSLLKKPDGVKESTESSNTTIEDEDVKARKQEIIKVTEQLIEAINNGDFEAYTKICDPGLTAFEPEALGNLVEGMDFHRFYFENALSKSNKPIHTIILNPHVHLVGDDAACIAYIRLTQYMDGSGMPKTMQSEETRVWHRRDGKWQNVHFHRSGSPTVPIKPPCIPNGKENFSGGTSLWQNI

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Sheep
100
Pig
91
Dog
91
Rabbit
91
Horse
67
Bovine
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13557 作为底物

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
K43 Ubiquitination
K57 Ubiquitination
K69 Ubiquitination
S79 Phosphorylation
K138 Ubiquitination
S146 Phosphorylation
K227 Acetylation
K227 Ubiquitination
Y231 Phosphorylation
S235 Phosphorylation
K246 Ubiquitination
K251 Ubiquitination
T254 Phosphorylation
S264 Phosphorylation
S276 Phosphorylation
S280 Phosphorylation
T287 Phosphorylation Q13557 (CAMK2D)
K299 Methylation
K301 Methylation
T306 Phosphorylation
T307 Phosphorylation
T311 Phosphorylation
S315 Phosphorylation
K318 Acetylation
K318 Ubiquitination
S319 Phosphorylation
S330 Phosphorylation
T331 Phosphorylation
S333 Phosphorylation
S334 Phosphorylation
T336 Phosphorylation
T337 Phosphorylation
C373 S-Nitrosylation
S404 Phosphorylation
S441 Phosphorylation
S472 Phosphorylation
T474 Phosphorylation
S490 Phosphorylation

翻译修饰 - Q13557 作为激酶

Substrate Site Source
O15084 (ANKRD28) S1011 Uniprot
P08670 (VIM) S83 Uniprot
P13688-8 (CEACAM1) T457 Uniprot
P13688-8 (CEACAM1) S459 Uniprot
P16220 (CREB1) S133 Uniprot
P16220 (CREB1) S142 Uniprot
P26678 (PLN) T17 Uniprot
P55327 (TPD52) S176 Uniprot
P56524 (HDAC4) S210 Uniprot
P56524 (HDAC4) S632 Uniprot
Q00613 (HSF1) S230 Uniprot
Q03060-16 (CREM) S87 Uniprot
Q08289-2 (CACNB2) T499 Uniprot
Q08289 (CACNB2) T554 Uniprot
Q13557 (CAMK2D) T287 Uniprot
Q13557-1 (CAMK2D) S315 Uniprot
Q13557-8 (CAMK2D) S319 Uniprot
Q13557-1 (CAMK2D) T331 Uniprot
Q13557-1 (CAMK2D) S333 Uniprot
Q13557-1 (CAMK2D) S334 Uniprot
Q13557-8 (CAMK2D) T337 Uniprot
Q14524 (SCN5A) S516 Uniprot
Q14524 (SCN5A) T594 Uniprot
Q14571 (ITPR2) S150 Uniprot
Q14654 (KCNJ11) T224 Uniprot
Q16695 (HIST3H3) S11 Uniprot
Q8WYL5 (SSH1) S937 Uniprot
Q8WYL5 (SSH1) S978 Uniprot
Q8WZ42 (TTN) S4062 Uniprot
Q8WZ42 (TTN) S11878 Uniprot
Q8WZ42 (TTN) S12022 Uniprot
Q92736 (RYR2) S2814 Uniprot
Q9UQL6 (HDAC5) S498 Uniprot

研究背景

功能:

Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor.

翻译修饰:

Autophosphorylation of Thr-287 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-287 locks the kinase into an activated state.

细胞定位:

Cell membrane>Sarcolemma>Peripheral membrane protein>Cytoplasmic side. Sarcoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.

亚基结构:

CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other. Interacts with RRAD and CACNB2 (By similarity).

蛋白家族:

The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization.

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Amphetamine addiction.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Environmental adaptation > Circadian entrainment.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Nervous system > Cholinergic synapse.

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Gastric acid secretion.

限制条款

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