Cytokeratin 19 Antibody - #AF0192

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产品: Cytokeratin 19 抗体
货号: AF0192
描述: Rabbit polyclonal antibody to Cytokeratin 19
应用: WB IHC IF/ICC IHC-F
文献验证: WB, IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Rabbit, Chicken
蛋白号: P08727
RRID: AB_2833385

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Cytokeratin 19 Antibody detects endogenous levels of total Cytokeratin 19.
RRID:
AB_2833385
引用格式: Affinity Biosciences Cat# AF0192, RRID:AB_2833385.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

40 kDa keratin intermediate filament; CK 19; CK-19; CK19; Cytokeratin 19; Cytokeratin-19; K19; K1C19_HUMAN; K1CS; Keratin 19; Keratin type I 40 kD; Keratin type I 40kD; Keratin type I cytoskeletal 19; Keratin, type I cytoskeletal 19; Keratin, type I, 40 kd; Keratin-19; KRT19; MGC15366;

抗原和靶标

免疫原:

A synthesized peptide derived from human Cytokeratin 19, corresponding to a region within the internal amino acids.

基因/基因ID:
KRT19(3880)
描述:
K19 a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 19 differs from all other IF proteins in lacking the C-terminal tail domain.

研究领域

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

文献引用

1). BMSC loaded photo-crosslinked hyaluronic acid/collagen hydrogel incorporating FG4592 for enhanced cell proliferation and nucleus pulposus differentiation. International journal of biological macromolecules, 2024 (PubMed: 38834125) [IF=7.7]
2). Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2016 (PubMed: 27314916) [IF=5.7]

Application: IF/ICC    Species: rat    Sample:

Figure 5. Analysis of hepatic protein markers after 3 weeks of hepatic differentiation. (A) H&E staining of hepatic differentiation of mesenchymal stem cells (MSCs) in all groups. Immunofluorescence analysis of (B) alpha fetoprotein (AFP), (C) cytokeratin 19 (CK19) and (D) albumin (ALB) expression in all groups. Undifferentiated MSCs were used as controls. Scale bar, 100 µm. 2D, 2-dimensional; 3D, 3-dimensional; DLS, decellularized liver scaffold.
3). Hepatic differentiation of mouse bone marrow‑derived mesenchymal stem cells using a novel 3D culture system. Molecular Medicine Reports, 2017 (PubMed: 29152658) [IF=3.4]

Application: IF/ICC    Species: Mouse    Sample:

Figure 4. Analysis of hepatic protein markers in mouse MSCs following 23 days of hepatic differentiation. Green immunofluorescence staining of ALB, AFP and CK19 in undifferentiated MSC control, 2D and 3D groups. Nuclei were stained blue with DAPI. Scale bars, 100 µm. MSCs, mesenchymal stem cells; ALB, albumin; AFP, α-fetoprotein; CK19, cytokeratin-19.
4). Human umbilical cord mesenchymal stem cells implantation accelerates cutaneous wound healing in diabetic rats via the Wnt signaling pathway. EUROPEAN JOURNAL OF MEDICAL RESEARCH, 2019 (PubMed: 30736851) [IF=2.8]

Application: WB    Species: Human    Sample: hUCMSCs

Fig. 5 Activation of the Wnt signaling pathway enhanced hUCMSCs differentiation. a Western blotting was applied to detect the changes in the levels of β-catenin, c-Myc, p63, CK19, and PCNA proteins. b Statistical analysis of a results. c The differentiation degree of hUCMSCs was evaluated by cell morphology under optical microscope (scale: 100 μm). d Statistical analysis on the ratio of cells with round or small polygonal morphology displayed in c;

Application: WB    Species: Human    Sample: hUCMSCs

Fig. 5 Activation of the Wnt signaling pathway enhanced hUCMSCs differentiation. a Western blotting was applied to detect the changes in the levels of β-catenin, c-Myc, p63, CK19, and PCNA proteins. b Statistical analysis of a results. c The differentiation degree of hUCMSCs was evaluated by cell morphology under optical microscope (scale: 100 μm). d Statistical analysis on the ratio of cells with round or small polygonal morphology displayed in c;
5). Adenomyosis-derived extracellular vesicles endow endometrial epithelial cells with an invasive phenotype through epithelial-mesenchymal transition. Genes & Diseases, 2020 (PubMed: 33335963)

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