RPS15A Antibody - #DF9117
(1)
(6)
产品描述
来源:
Rabbit
应用:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
RPS15A Antibody detects endogenous levels of total RPS15A.
RRID:
AB_2842313
引用格式: Affinity Biosciences Cat# DF9117, RRID:AB_2842313.
引用格式: Affinity Biosciences Cat# DF9117, RRID:AB_2842313.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
40S ribosomal protein S15a; FLJ27457; MGC111208; OK/SW-cl.82; Ribosomal protein S15a; S15a; Upregulated by HBV X protein;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
序列:
- P62244 RS15A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P62244 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K12 | Ubiquitination | Uniprot | |
| K19 | Acetylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K32 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| T66 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K88 | Acetylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| T121 | Phosphorylation | Uniprot | |
| K124 | Acetylation | Uniprot | |
| K124 | Ubiquitination | Uniprot |
研究背景
功能:
Structural component of the ribosome. Required for proper erythropoiesis.
亚基结构:
Component of the 40S ribosomal subunit.
蛋白家族:
Belongs to the universal ribosomal protein uS8 family.
研究领域
· Genetic Information Processing > Translation > Ribosome.
文献引用
1). m6A‑modified HOXC10 promotes HNSCC progression via co‑activation of ADAM17/EGFR and Wnt/β‑catenin signaling. International journal of oncology
(PubMed: 38063205)
[IF=5.2]
Application: WB Species: Human Sample: HNSCC cells
Figure 5 HOXC10 facilitates Wnt/β-catenin signaling in HNSCC by interacting with RPS15A. (A-C) The binding partners of HOXC10 were analyzed by the combination of Co-IP and mass spectrometric analyses, and 11 proteins are presented. (D) Co-IP and western blot analysis were used to verify the interaction between HOXC10 and RPS15A. (E) Immunofluorescence was used to identify the colocalization of HOXC10 and RPS15A. Scale bar, 25 µm. (F and G) RT-qPCR and western blot analysis of RPS15A expression in HNSCC cells transfected with shRNA2 or HOXC10. (H) Ubiquitination assay for the effects of HOXC10 on RPS15A ubiquitination. (I) GSEA based on the TCGA dataset suggested that HOXC10 expression was positively associated with the activation of the Wnt/β-catenin pathway. (J) The efficiency of transfection in the indicated cells was confirmed by RT-qPCR and western blotting. (K) Immunofluorescence detection of β-catenin in the nucleus after transfection with shRNA2, HOXC10, RPS15A, or siRPS15A in indicated cells. Scale bar, 25 µm. (L) Western blot analysis was performed to detect β-catenin, Myc, MMP7, Axin2, and RPS15A protein in HNSCC cells transfected with shRNA2, HOXC10, RPS15A, or siRPS15A. (M) Myc, MMP7, and Axin2 mRNA levels were analyzed by RT-qPCR in transfected HNSCC cells. (N) The activity of the Wnt/β-catenin pathway was analyzed by TOP/FOP-Flash luciferase reporter assay. (O and P) Migration and invasion ability in transfected HNSCC cells were assessed by Transwell assay. Scale bar, 100 µm **P
Application: IF/ICC Species: Human Sample: HNSCC cells
Figure 5 HOXC10 facilitates Wnt/β-catenin signaling in HNSCC by interacting with RPS15A. (A-C) The binding partners of HOXC10 were analyzed by the combination of Co-IP and mass spectrometric analyses, and 11 proteins are presented. (D) Co-IP and western blot analysis were used to verify the interaction between HOXC10 and RPS15A. (E) Immunofluorescence was used to identify the colocalization of HOXC10 and RPS15A. Scale bar, 25 µm. (F and G) RT-qPCR and western blot analysis of RPS15A expression in HNSCC cells transfected with shRNA2 or HOXC10. (H) Ubiquitination assay for the effects of HOXC10 on RPS15A ubiquitination. (I) GSEA based on the TCGA dataset suggested that HOXC10 expression was positively associated with the activation of the Wnt/β-catenin pathway. (J) The efficiency of transfection in the indicated cells was confirmed by RT-qPCR and western blotting. (K) Immunofluorescence detection of β-catenin in the nucleus after transfection with shRNA2, HOXC10, RPS15A, or siRPS15A in indicated cells. Scale bar, 25 µm. (L) Western blot analysis was performed to detect β-catenin, Myc, MMP7, Axin2, and RPS15A protein in HNSCC cells transfected with shRNA2, HOXC10, RPS15A, or siRPS15A. (M) Myc, MMP7, and Axin2 mRNA levels were analyzed by RT-qPCR in transfected HNSCC cells. (N) The activity of the Wnt/β-catenin pathway was analyzed by TOP/FOP-Flash luciferase reporter assay. (O and P) Migration and invasion ability in transfected HNSCC cells were assessed by Transwell assay. Scale bar, 100 µm **P
Application: IHC Species: Human Sample: HNSCC cells
Figure 5 HOXC10 facilitates Wnt/β-catenin signaling in HNSCC by interacting with RPS15A. (A-C) The binding partners of HOXC10 were analyzed by the combination of Co-IP and mass spectrometric analyses, and 11 proteins are presented. (D) Co-IP and western blot analysis were used to verify the interaction between HOXC10 and RPS15A. (E) Immunofluorescence was used to identify the colocalization of HOXC10 and RPS15A. Scale bar, 25 µm. (F and G) RT-qPCR and western blot analysis of RPS15A expression in HNSCC cells transfected with shRNA2 or HOXC10. (H) Ubiquitination assay for the effects of HOXC10 on RPS15A ubiquitination. (I) GSEA based on the TCGA dataset suggested that HOXC10 expression was positively associated with the activation of the Wnt/β-catenin pathway. (J) The efficiency of transfection in the indicated cells was confirmed by RT-qPCR and western blotting. (K) Immunofluorescence detection of β-catenin in the nucleus after transfection with shRNA2, HOXC10, RPS15A, or siRPS15A in indicated cells. Scale bar, 25 µm. (L) Western blot analysis was performed to detect β-catenin, Myc, MMP7, Axin2, and RPS15A protein in HNSCC cells transfected with shRNA2, HOXC10, RPS15A, or siRPS15A. (M) Myc, MMP7, and Axin2 mRNA levels were analyzed by RT-qPCR in transfected HNSCC cells. (N) The activity of the Wnt/β-catenin pathway was analyzed by TOP/FOP-Flash luciferase reporter assay. (O and P) Migration and invasion ability in transfected HNSCC cells were assessed by Transwell assay. Scale bar, 100 µm **P
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