产品: | 磷酸化 TrkA (Tyr680+Tyr681) 抗体 |
货号: | AF3072 |
描述: | Rabbit polyclonal antibody to Phospho-TrkA (Tyr680+Tyr681) |
应用: | WB IHC |
反应: | Human, Mouse, Rat |
预测: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken |
分子量: | 87kDa; 87kD(Calculated). |
蛋白号: | P04629 |
RRID: | AB_2834509 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3072, RRID:AB_2834509.
展开/折叠
gp140trk; High affinity nerve growth factor receptor; High affinity nerve growth factor receptor precursor; MTC; Neurotrophic tyrosine kinase receptor type 1; NTRK1; NTRK1_HUMAN; Oncogene TRK; p14-TrkA; p140 TrkA; p140-TrkA; Slow nerve growth; Trk A; TRK; Trk-A; TRK1; TRK1-transforming tyrosine kinase protein; Tropomyosin-related kinase A; Tyrosine kinase receptor A; Tyrosine kinase receptor;
抗原和靶标
Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
- P04629 NTRK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLRGGRRGQLGWHSWAAGPGSLLAWLILASAGAAPCPDACCPHGSSGLRCTRDGALDSLHHLPGAENLTELYIENQQHLQHLELRDLRGLGELRNLTIVKSGLRFVAPDAFHFTPRLSRLNLSFNALESLSWKTVQGLSLQELVLSGNPLHCSCALRWLQRWEEEGLGGVPEQKLQCHGQGPLAHMPNASCGVPTLKVQVPNASVDVGDDVLLRCQVEGRGLEQAGWILTELEQSATVMKSGGLPSLGLTLANVTSDLNRKNVTCWAENDVGRAEVSVQVNVSFPASVQLHTAVEMHHWCIPFSVDGQPAPSLRWLFNGSVLNETSFIFTEFLEPAANETVRHGCLRLNQPTHVNNGNYTLLAANPFGQASASIMAAFMDNPFEFNPEDPIPVSFSPVDTNSTSGDPVEKKDETPFGVSVAVGLAVFACLFLSTLLLVLNKCGRRNKFGINRPAVLAPEDGLAMSLHFMTLGGSSLSPTEGKGSGLQGHIIENPQYFSDACVHHIKRRDIVLKWELGEGAFGKVFLAECHNLLPEQDKMLVAVKALKEASESARQDFQREAELLTMLQHQHIVRFFGVCTEGRPLLMVFEYMRHGDLNRFLRSHGPDAKLLAGGEDVAPGPLGLGQLLAVASQVAAGMVYLAGLHFVHRDLATRNCLVGQGLVVKIGDFGMSRDIYSTDYYRVGGRTMLPIRWMPPESILYRKFTTESDVWSFGVVLWEIFTYGKQPWYQLSNTEAIDCITQGRELERPRACPPEVYAIMRGCWQREPQQRHSIKDVHARLQALAQAPPVYLDVLG
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P04629 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
N95 | N-Glycosylation | Uniprot | |
N121 | N-Glycosylation | Uniprot | |
S123 | Phosphorylation | Uniprot | |
N188 | N-Glycosylation | Uniprot | |
N262 | N-Glycosylation | Uniprot | |
N281 | N-Glycosylation | Uniprot | |
N358 | N-Glycosylation | Uniprot | |
Y490 | Phosphorylation | Uniprot | |
Y496 | Phosphorylation | P04629 (NTRK1) | Uniprot |
Y591 | Phosphorylation | Uniprot | |
Y670 | Phosphorylation | Uniprot | |
S672 | Phosphorylation | Uniprot | |
Y674 | Phosphorylation | Uniprot | |
Y675 | Phosphorylation | Uniprot | |
Y676 | Phosphorylation | P04629 (NTRK1) | Uniprot |
Y680 | Phosphorylation | P04629 (NTRK1) | Uniprot |
Y681 | Phosphorylation | P04629 (NTRK1) | Uniprot |
Y701 | Phosphorylation | P04629 (NTRK1) | Uniprot |
Y729 | Phosphorylation | Uniprot | |
Y757 | Phosphorylation | Uniprot | |
Y785 | Phosphorylation | Uniprot | |
Y791 | Phosphorylation | P04629 (NTRK1) | Uniprot |
翻译修饰 - P04629 作为激酶
Substrate | Site | Source |
---|---|---|
P04629-3 (NTRK1) | Y460 | Uniprot |
P04629-2 (NTRK1) | Y490 | Uniprot |
P04629 (NTRK1) | Y496 | Uniprot |
P04629-2 (NTRK1) | Y670 | Uniprot |
P04629-2 (NTRK1) | Y674 | Uniprot |
P04629-2 (NTRK1) | Y675 | Uniprot |
P04629 (NTRK1) | Y676 | Uniprot |
P04629 (NTRK1) | Y680 | Uniprot |
P04629 (NTRK1) | Y681 | Uniprot |
P04629 (NTRK1) | Y701 | Uniprot |
P04629-2 (NTRK1) | Y751 | Uniprot |
P04629-3 (NTRK1) | Y755 | Uniprot |
P04629-2 (NTRK1) | Y785 | Uniprot |
P04629 (NTRK1) | Y791 | Uniprot |
P05067 (APP) | Y757 | Uniprot |
P29353-2 (SHC1) | Y317 | Uniprot |
P35222 (CTNNB1) | Y142 | Uniprot |
Q92529-2 (SHC3) | Y218 | Uniprot |
Q92529-2 (SHC3) | Y219 | Uniprot |
Q92529-2 (SHC3) | Y256 | Uniprot |
Q92529-2 (SHC3) | Y257 | Uniprot |
Q92529-2 (SHC3) | Y283 | Uniprot |
Q92529-2 (SHC3) | Y301 | Uniprot |
研究背景
Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand. Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival (By similarity). Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
Resistant to NGF, it constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1.
N-glycosylated. Isoform TrkA-I and isoform TrkA-II are N-glycosylated.
Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination by NEDD4L leads to degradation. Ubiquitination regulates the internalization of the receptor (By similarity).
Cell membrane>Single-pass type I membrane protein. Early endosome membrane>Single-pass type I membrane protein. Late endosome membrane>Single-pass type I membrane protein. Recycling endosome membrane>Single-pass type I membrane protein.
Note: Rapidly internalized after NGF binding (PubMed:1281417). Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes.
Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Homodimerization is induced by binding of a NGF dimer. Interacts with SQSTM1; bridges NTRK1 to NGFR. Forms a ternary complex with NGFR and KIDINS220; this complex is affected by the expression levels of KIDINS220 and an increase in KIDINS220 expression leads to a decreased association of NGFR and NTRK1 (By similarity). Interacts with SH2D1A; regulates NTRK1 (By similarity). Interacts (phosphorylated upon activation by NGF) with SHC1; mediates SHC1 phosphorylation and activation. Interacts (phosphorylated upon activation by NGF) with PLCG1; mediates PLCG1 phosphorylation and activation. Interacts (phosphorylated) with SH2B1 and SH2B2 (By similarity). Interacts with GRB2. Interacts with PIK3R1. Interacts with FRS2. Interacts with SORT1; may regulate NTRK1 anterograde axonal transport. Interacts with RAB7A (By similarity). Found in a complex, at least composed of KIDINS220, MAGI2, NTRK1 and RAPGEF2; the complex is mainly formed at late endosomes in a nerve growth factor (NGF)-dependent manner (By similarity). Interacts with RAPGEF2; the interaction is strengthened after NGF stimulation (By similarity). Interacts with PTPRS (By similarity). Interacts with USP36; USP36 does not deubiquitinate NTRK1. Interacts with GGA3.
The transmembrane domain mediates interaction with KIDINS220.
The extracellular domain mediates interaction with NGFR.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
研究领域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Specific types > Thyroid cancer. (View pathway)
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels. (View pathway)
文献引用
Application: WB Species: mouse Sample: striatum
限制条款
产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.