GOLT1B Antibody - #DF9071

Fig. 2 GOLT1B promotes CRC cell proliferation, invasion and migration. a, b GOLT1B mRNA and protein level were determined by qPCR and western blot in HCT116 and RKO cells. c, d Cell migration were determined by wound healing experiment after siRNA transfection or overexpression of GOLT1B, respectively. e, f Cell invasion were detected by transwell assays with matrigel after siRNA transfection or overexpression of GOLT1B, respectively. g Effect of GOLT1B on proliferation of colorectal cancer cells in vitro. All data are shown as mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Fig. 3 GOLT1B promotes metastasis of colorectal cancer in vivo. a GOLT1B stable overexpression was verified by qPCR and western blot. b The migration and invasion ability of HCT116 cells stablely transfected with control vector or GOLT1B were determined by transwell assays in vitro. c The average bioluminescence imaging signals in mice bearing liver metastatic burden was determined by IVIS imaging (HCT116Vector vs. HCT116GOLT1B, n = 8). d Body weight curves of mice in two groups. e Representative images of metastatic foci (pointed out with red arrow) in mice liver. f The number of liver metastatic foci in control vector and GOLT1B group. g IHC staining of GOLT1B and H&E staining in liver metastatic loci

Fig. 5 GOLT1B interacts with DVL2 to promote it transport and localization to the cell membrane. a The nucleus and cytoplasm separation experiment showed that GOLT1B overexpression increased the level of nuclear β-catenin. b The cell membrane and cytoplasm separation experiment showed that GOLT1B could induce DVL2 upregualtion on the cell membrane. c CO-IP experiment confirmed the interaction between GOLT1B and DVL2. d Immunofluorescence experiment showed that GOLT1B and DVL2 were co-localized

Fig. 2 |GOLT1B promotes CRC cell proliferation, invasion and migration. a, b GOLT1B mRNA and protein level were determined by qPCR and western blot in HCT116 and RKO cells.