KRT3/6A/75/76 Antibody - #DF9009

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产品: KRT3/6A/75/76 抗体
货号: DF9009
描述: Rabbit polyclonal antibody to KRT3/6A/75/76
应用: WB IHC IF/ICC
文献验证: WB
反应: Human
预测: Horse, Dog
分子量: 64 kDa; 66kD,64kD,60kD(Calculated).
蛋白号: Q01546 | P12035 | P02538 | O95678
RRID: AB_2842205

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 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Horse(100%), Dog(%)
克隆:
Polyclonal
特异性:
KRT3/6A/75/76 Antibody detects endogenous levels of total KRT3/6A/75/76.
RRID:
AB_2842205
引用格式: Affinity Biosciences Cat# DF9009, RRID:AB_2842205.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CK 2P; CK-2P; CK2P; Cytokeratin 2P; Cytokeratin-2P; Cytokeratin2; Cytokeratin2P; HUMCYT 2A; HUMCYT2A; K22O_HUMAN; K2P; K76; KB9; Keratin 2p; Keratin 76; Keratin 76, type II; Keratin; Keratin type II cytoskeletal 2 oral; Keratin-76; Keratin2p; Keratin76; KRT 2B; KRT 2P; KRT 76; KRT2B; KRT2P; KRT76; type II cytoskeletal 2 oral; Type-II keratin Kb9; 65 kDa cytokeratin; CK-3; CK3; Cytokeratin-3; K2C3_HUMAN; K3; Keratin 3; Keratin; Keratin type II cytoskeletal 3; Keratin-3; KRT3; type II cytoskeletal 3; Type-II keratin Kb3; CK 4; CK 5; CK 6A; CK 6B; CK 6C; CK 6D; CK 6E; CK4; CK5; CK6A; CK6B; CK6C; CK6D; CYK4; Cytokeratin 4; Cytokeratin 5; Cytokeratin 6A; Cytokeratin 6B; Cytokeratin 6C; Cytokeratin 6D; Cytokeratin 6E; DDD; K4; K5; K6A; K6a keratin; K6B; K6C; K6D; Keratin 5; Keratin 6A; Keratin 6B; Keratin 6C; Keratin 6D; Keratin 6E; Keratin K6h; Keratin, epidermal type II, K6A; Keratin, epidermal type II, K6C; Keratin, epidermal, type II, K6B; Keratin, type II cytoskeletal 4; Keratin, type II cytoskeletal 5; Keratin, type II cytoskeletal 6B; Keratin, type II cytoskeletal 6D; KRT5A; KRT6C; KRT6D; KRT6E; CK-75; CK75; Cytokeratin 75; Cytokeratin-75; hK6hf; K2C75_HUMAN; K6HF; K75; KB18; Keratin 6 hair follicle; Keratin 75; Keratin; Keratin type II cytoskeletal 75; Keratin-6 hair follicle; Keratin-75; Krt75; PFB; type II cytoskeletal 75; Type II keratin 18; Type II keratin-K6hf; Type-II keratin Kb18;

抗原和靶标

免疫原:
Uniprot:

Q01546(K22O_HUMAN) >>Visit HPA database.

P12035(K2C3_HUMAN) >>Visit HPA database.

P02538(K2C6A_HUMAN) >>Visit HPA database.

O95678(K2C75_HUMAN) >>Visit HPA database.

基因/基因ID:
KRT76(51350) | KRT3(3850) | KRT75(9119) | KRT6A(3853)
表达:
P12035 K2C3_HUMAN:

Cornea specific.

P02538 K2C6A_HUMAN:

Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

O95678 K2C75_HUMAN:

Highly expressed in hair follicles from scalp. Specifically expressed in the of the hair companion layer follicle, a single layered band of flat and vertically oriented cells between the cuboidal outer root sheath (ORS) cells and the inner root sheath (IRS) that stretches from the lowermost bulb region to the isthmus of the follicle. Also expressed in medullated hairs. In nails, it is almost exclusively present in the nail bed (at protein level).

序列:
MNRQVCKKSFSGRSQGFSGRSAVVSGSSRMSCVARSGGAGGGACGFRSGAGSFGSRSLYNLGSNKSISISVAAGSSRAGGFGGGRSSCGFAGGYGGGFGGSYGGGFGGGRGVGSGFGGAGGFGGAGGFGGPGVFGGPGSFGGPGGFGPGGFPGGIQEVIVNQSLLQPLNVEIDPQIGQVKAQEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWELLQQQTTGSGPSSLEPCFESYISFLCKQLDSLLGERGNLEGELKSMQDLVEDFKKKYEDEINKRTAAENEFVGLKKDVDAAFMNKVELQAKVDSLTDEVSFLRTLYEMELSQMQSHASDTSVVLSMDNNRCLDLGSIIAEVRAQYEEIAQRSKSEAEALYQTKLGELQTTAGRHGDDLRNTKSEIMELNRMIQRLRAEIENVKKQNANLQTAIAEAEQRGEMALKDANAKLQDLQTALQKAKDDLARLLRDYQELMNVKLALDVEIATYRKLLEGEECRMSGECQSAVCISVVSNVTSTSGSSGSSRGVFGGVSGSGSGGYKGGSSSSSSSGYGVSGGSGSGYGGVSSGSTGGRGSSGSYQSSSSGSRLGGAGSISVSHSGMGSSSGSIQTSGGSGYKSGGGGSTSIRFSQTTSSSQHSSTK

MSRQASKTSGGGSQGFSGRSAVVSGSSRMSCVAHSGGAGGGAYGFRSGAGGFGSRSLYNLGGNKSISISVAAGGSRAGGFGGGRSSCAFAGGYGGGFGSGYGGGFGGGFGGGRGMGGGFGGAGGFGGAGGFGGAGGFGGPGGFGGSGGFGGPGSLGSPGGFGPGGFPGGIQEVTINQSLLQPLNVEIDPQIGQVKAQEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWNLLQQQGTSSISGTNNLEPLFENHINYLRSYLDNILGERGRLDSELKNMEDLVEDFKKKYEDEINKRTAAENEFVTLKKDVDSAYMNKVELQAKVDALIDEIDFLRTLYDAELSQMQSHISDTSVVLSMDNNRSLDLDSIIAEVRAQYEDIAQRSKAEAEALYQTKLGELQTTAGRHGDDLRNTKSEIIELNRMIQRLRAEIEGVKKQNANLQTAIAEAEQHGEMALKDANAKLQELQAALQQAKDDLARLLRDYQELMNVKLALDVEIATYRKLLEGEEYRMSGECPSAVSISVVSSSTTSASAGGYGGGYGGGMGGGLGGGFSAGGGSGSGFGRGGGGGIGGGFGGGSSGFSGGSGFGSISGARYGVSGGGFSSASNRGGSIKFSQSSQSSQRYSR

MASTSTTIRSHSSSRRGFSANSARLPGVSRSGFSSVSVSRSRGSGGLGGACGGAGFGSRSLYGLGGSKRISIGGGSCAISGGYGSRAGGSYGFGGAGSGFGFGGGAGIGFGLGGGAGLAGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPTIQRVRAEEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWTLLQEQGTKTVRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRGMQDLVEDFKNKYEDEINKRTAAENEFVTLKKDVDAAYMNKVELQAKADTLTDEINFLRALYDAELSQMQTHISDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIAQRSRAEAESWYQTKYEELQVTAGRHGDDLRNTKQEIAEINRMIQRLRSEIDHVKKQCANLQAAIADAEQRGEMALKDAKNKLEGLEDALQKAKQDLARLLKEYQELMNVKLALDVEIATYRKLLEGEECRLNGEGVGQVNISVVQSTVSSGYGGASGVGSGLGLGGGSSYSYGSGLGVGGGFSSSSGRAIGGGLSSVGGGSSTIKYTTTSSSSRKSYKH

MSRQSSITFQSGSRRGFSTTSAITPAAGRSRFSSVSVARSAAGSGGLGRISSAGASFGSRSLYNLGGAKRVSINGCGSSCRSGFGGRASNRFGVNSGFGYGGGVGGGFSGPSFPVCPPGGIQEVTVNQSLLTPLHLQIDPTIQRVRAEEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWALLQEQGSRTVRQNLEPLFDSYTSELRRQLESITTERGRLEAELRNMQDVVEDFKVRYEDEINKRTAAENEFVALKKDVDAAYMNKVELEAKVKSLPEEINFIHSVFDAELSQLQTQVGDTSVVLSMDNNRNLDLDSIIAEVKAQYEDIANRSRAEAESWYQTKYEELQVTAGRHGDDLRNTKQEISEMNRMIQRLRAEIDSVKKQCSSLQTAIADAEQRGELALKDARAKLVDLEEALQKAKQDMARLLREYQELMNIKLALDVEIATYRKLLEGEECRLSGEGVSPVNISVVTSTLSSGYGSGSSIGGGNLGLGGGSGYSFTTSGGHSLGAGLGGSGFSATSNRGLGGSGSSVKFVSTTSSSQKSYTH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Dog
100
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Probably contributes to terminal cornification.

亚基结构:

Heterotetramer of two type I and two type II keratins.

蛋白家族:

Belongs to the intermediate filament family.

组织特异性:

Cornea specific.

亚基结构:

Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.

蛋白家族:

Belongs to the intermediate filament family.

功能:

Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.

组织特异性:

Constitutively expressed in distinct types of epithelia such as those in oral mucosa, esophagus, papillae of tongue and hair follicle outer root sheath.

亚基结构:

Heterodimer of a type I and a type II keratin. KRT6 isomers associate with KRT16 and/or KRT17 (By similarity). Interacts with TCHP.

蛋白家族:

Belongs to the intermediate filament family.

功能:

Plays a central role in hair and nail formation. Essential component of keratin intermediate filaments in the companion layer of the hair follicle.

组织特异性:

Highly expressed in hair follicles from scalp. Specifically expressed in the of the hair companion layer follicle, a single layered band of flat and vertically oriented cells between the cuboidal outer root sheath (ORS) cells and the inner root sheath (IRS) that stretches from the lowermost bulb region to the isthmus of the follicle. Also expressed in medullated hairs. In nails, it is almost exclusively present in the nail bed (at protein level).

亚基结构:

Heterodimer of a type I and a type II keratin. May associate with KRT17.

蛋白家族:

Belongs to the intermediate filament family.

文献引用

1). Exosome-derived long non-coding RNA AC010789.1 modified by FTO and hnRNPA2B1 accelerates growth of hair follicle stem cells against androgen alopecia by activating S100A8/Wnt/β-catenin signalling. Clinical and translational medicine, 2025 (PubMed: 39748192) [IF=7.9]

Application: WB    Species: Mouse    Sample: HFSCs

FIGURE 1 Long non-coding RNA (lncRNA) AC010789.1 was downregulated in hair follicle tissues from androgenic alopecia (AGA) and upregulated in hair follicle stem cells (HFSCs). (A) Real-time quantitative polymerase chain reaction (qRT-PCR) analysis of the expression levels of AC010789.1 in 10 pairs of AGA hair follicle tissues. (B) qRT-PCR analysis of the expression levels of AC010789.1 in HFSCs. (C) 5-ethynyl-2'-deoxyuridine assay (EdU) flow cytometry analysis of the molecular markers CD29 and CD71 of HFSCs. (D) qRT-PCR analysis of the expression levels of AC010789.1 after the transfection with AC010789.1 OE lentiviruses into HFSCs and K6HF and Lgr5 after the transfection with AC010789.1 OE lentiviruses into HFSCs. (E) Western blot analysis of the expression levels of AC010789.1, K6HF and Lgr5 after the transfection with AC010789.1 OE lentiviruses into HFSCs. (F) qRT-PCR analysis of the expression levels of AC010789.1 after transfection with si-AC010789.1 into HFSCs and K6HF and Lgr5 after transfection with si-AC010789.1 into HFSCs. (G) Western blot analysis of the expression levels of AC010789.1, K6HF and Lgr5 after transfection with si-AC010789.1 into HFSCs. Data shown are the mean ± SEM of three experiments. *p < .05, **p < .01 and ***p < .001.

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