IFI27 Antibody - #DF8989

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产品: IFI27 抗体
货号: DF8989
描述: Rabbit polyclonal antibody to IFI27
应用: WB IHC
文献验证: WB, IHC
反应: Human
分子量: 11 kDa; 12kD(Calculated).
蛋白号: P40305
RRID: AB_2842185

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
IFI27 Antibody detects endogenous levels of total IFI27.
RRID:
AB_2842185
引用格式: Affinity Biosciences Cat# DF8989, RRID:AB_2842185.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2310061N23Rik; FAM 14D; FAM14D; IFI 27; IFI27; IFI27_HUMAN; Interferon alpha induced 11.5 kDa protein; Interferon alpha inducible protein 27; Interferon alpha-induced 11.5 kDa protein; Interferon alpha-inducible protein 27; interferon alpha-inducible protein 27, mitochondrial; Interferon inducible protein 27; interferon-stimulated gene 12; Interferon-stimulated gene 12a protein; ISG 12; ISG12; ISG12(a); ISG12(a) protein; ISG12A; mitochondrial; p27;

抗原和靶标

免疫原:
Uniprot:

P40305(IFI27_HUMAN) >>Visit HPA database.

基因/基因ID:
IFI27(3429)
序列:
MEASALTSSAVTSVAKVVRVASGSAVVLPLARIATVVIGGVVAMAAVPMVLSAMGFTAAGIASSSIAAKMMSAAAIANGGGVASGSLVATLQSLGATGLSGLTKFILGSIGSAIAAVIARFY

研究背景

功能:

Probable adapter protein involved in different biological processes. Part of the signaling pathways that lead to apoptosis. Involved in type-I interferon-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9. Also functions in TNFSF10-induced apoptosis. May also have a function in the nucleus, where it may be involved in the interferon-induced negative regulation of the transcriptional activity of NR4A1, NR4A2 and NR4A3 through the enhancement of XPO1-mediated nuclear export of these nuclear receptors. May thereby play a role in the vascular response to injury (By similarity). In the innate immune response, has an antiviral activity towards hepatitis C virus/HCV. May prevent the replication of the virus by recruiting both the hepatitis C virus non-structural protein 5A/NS5A and the ubiquitination machinery via SKP2, promoting the ubiquitin-mediated proteasomal degradation of NS5A.

细胞定位:

Mitochondrion membrane>Multi-pass membrane protein. Nucleus inner membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: Exclusive localizations in either the nucleus or the mitochondrion have been reported.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer. Interacts with hepatitis C virus/HCV non-structural protein NS5A; promotes the ubiquitin-mediated proteasomal degradation of NS5A. Interacts with SKP2; promotes the ubiquitin-mediated proteasomal degradation of NS5A. Interacts with NR4A1. May interact with BCL2.

蛋白家族:

Belongs to the IFI6/IFI27 family.

文献引用

1). Single cell-spatial transcriptomics and bulk multi-omics analysis of heterogeneity and ecosystems in hepatocellular carcinoma. NPJ precision oncology, 2024 (PubMed: 39548284) [IF=7.9]

Application: IHC    Species: human    Sample: HCC cells

Fig. 3: Spatiotemporal intratumor heterogeneities in HCC and cirrhosis. a UMAP plot showing the HCC and CIR cell types, with different color-codes denoting lesions origin (left) and cell subclusters (right). b, c UMAP plot and spatial feature plots showing the distribution of specific markers on the cell subclusters from Fig. 2A (right). d Immunohistochemical validation of the expression of specific markers, the scale bars represent 20 μm. e Heatmap representing the average expression of specific markers of each cell subcluster at the full-length transcriptome, whole transcriptome, proteome, and scRNA levels. f Heatmap representing the levels of metabolic changes in the tissues and blood of HCC patients and control donor. See also Supplementary Table 1.
2). Integrated Bioinformatics and Validation Reveal IFI27 and Its Related Molecules as Potential Identifying Genes in Liver Cirrhosis. Biomolecules, 2023 (PubMed: 38275754) [IF=5.5]

Application: WB    Species: Mouse    Sample: Liver tissue

Figure 8. (A–C) Representative H&E staining, Masson staining and Sirius red staining of mice in the control group and the TAA group, scale: 100 µm. (D) Liver tissue immunofluorescence shows co-localization of IFI27 and liver macrophages. (E,F) The expression of COX7A1 and IFI27 in liver tissue and macrophages. (G) After treatment with si-IFI27, the knockdown efficiency was detected using RT-PCR. (H) ELISA was used to detect the concentration of IL-1β and TNF-α secreted from RAW264.7 in the different groups. (I–M) mRNA expression of M1 markers (CD80, CD86, iNOs) and pro-inflammation factors (IL-1β and TNF-α) in three groups were assayed using RT-qPCR. Data are presented as means ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. COX7A1, cytochrome c oxidase subunit 7A1; IFI27, interferon alpha-inducible protein 27; IL-1β, interleukin-1 beta; TNF-α, tumor necrosis factor alpha. (N) ROS generation level of RAW264.7 cells after LPS and si-IFI27 intervention (scale bar: 200 μm). (O) Quantification of ROS-positive area.
3). Prognostic value of IFI27 in HNSCC and functional analysis under ALKBH5 regulation. Scientific reports, 2025 (PubMed: 39994291) [IF=3.8]

Application: IHC    Species: human    Sample: HNSCC

Fig. 3 Expression of IFI27 in HNSCC. (A) IHC staining of IFI27 in HNSCC (10 × 20). (B) IHC staining of IFI27 in HNSCC (10 × 40). (C) Differential expression of IFI27 in HNSCC and adjacent normal tissues (P 

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