产品: 磷酸化 ADRB2 (Ser346) 抗体
货号: AF3117
描述: Rabbit polyclonal antibody to Phospho-ADRB2 (Ser346)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Bovine, Rabbit, Dog
分子量: 40kDa; 46kD(Calculated).
蛋白号: P07550
RRID: AB_2834498

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-ADRB2 (Ser346) Antibody detects endogenous levels of ADRB2 only when phosphorylated at Serine 346.
RRID:
AB_2834498
引用格式: Affinity Biosciences Cat# AF3117, RRID:AB_2834498.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADRB2; ADRB2_HUMAN; ADRB2R; ADRBR; Adrenergic beta 2 receptor surface; Adrenoceptor beta 2 surface; B2AR; BAR; beta 2 adrenoceptor; Beta 2 adrenoreceptor; Beta-2 adrenergic receptor; Beta-2 adrenoceptor; Beta-2 adrenoreceptor; BETA2AR; Catecholamine receptor; OTTHUMP00000160386;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2.
序列:
MGQPGNGSAFLLAPNGSHAPDHDVTQERDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSQGRNCSTNDSLL

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Pig
0
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P07550 作为底物

Site PTM Type Enzyme
Phosphorylation
Ubiquitination
T68 Phosphorylation
Y132 Phosphorylation P08069 (IGF1R) , P06213 (INSR)
Y141 Phosphorylation P06213 (INSR) , P08069 (IGF1R)
Y219 Phosphorylation
S246 Phosphorylation
S261 Phosphorylation Q05655 (PRKCD)
S262 Phosphorylation Q05655 (PRKCD)
Y308 Phosphorylation
Y326 Phosphorylation
S345 Phosphorylation P31749 (AKT1) , P17612 (PRKACA) , Q05655 (PRKCD)
S346 Phosphorylation P17612 (PRKACA) , Q05655 (PRKCD) , P31749 (AKT1)
Y350 Phosphorylation P06213 (INSR)
Y354 Phosphorylation P06213 (INSR)
S355 Phosphorylation P43250 (GRK6) , P35626 (GRK3) , P25098 (GRK2) , P34947 (GRK5)
S356 Phosphorylation P43250 (GRK6) , P25098 (GRK2) , P35626 (GRK3) , P34947 (GRK5)
T360 Phosphorylation
S364 Phosphorylation
T384 Phosphorylation P25098 (GRK2) , P34947 (GRK5)
T393 Phosphorylation P34947 (GRK5)
S396 Phosphorylation P25098 (GRK2) , P34947 (GRK5)
S401 Phosphorylation P25098 (GRK2) , P34947 (GRK5)
S407 Phosphorylation P25098 (GRK2) , P34947 (GRK5)
T408 Phosphorylation
S411 Phosphorylation P25098 (GRK2) , P34947 (GRK5)

研究背景

功能:

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.

翻译修饰:

Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.

Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK.

Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.

Polyubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. Ubiquitination by the VHL-E3 ligase complex is oxygen-dependent.

Hydroxylation by EGLN3 occurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2.

细胞定位:

Cell membrane>Multi-pass membrane protein. Early endosome.
Note: Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds SLC9A3R1 and GPRASP1. Interacts with ARRB1 and ARRB2. Interacts with SRC, USP20 and USP33. Interacts with VHL; the interaction, which is increased on hydroxylation of ADRB2, ubiquitinates ADRB2 leading to its degradation. Interacts with EGLN3; the interaction hydroxylates ADRB2 facilitating VHL-E3 ligase-mediated ubiquitination. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane. Interacts with CNIH4. Interacts with ARRDC3.

蛋白家族:

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.

研究领域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

· Organismal Systems > Digestive system > Salivary secretion.

文献引用

1). Jiang X et al. Kidney Yang Deficiency Syndrome Exacerbates Aβ25-35-Induced Pathological Changes, and Ginsenoside Re Ameliorates Synapse Lesions in Aβ25-35- Injected Rats with Kidney Yang Deficiency Syndrome. Current Alzheimer Research 2023 May 12; (PubMed: 37183470) [IF=2.1]

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