IFIT2 Antibody - #DF8962

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产品: IFIT2 抗体
货号: DF8962
描述: Rabbit polyclonal antibody to IFIT2
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Rat
预测: Pig
蛋白号: P09913
RRID: AB_2842158

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   规格 价格 库存
 50ul RMB¥ 1500 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Rat
克隆:
Polyclonal
特异性:
IFIT2 Antibody detects endogenous levels of total IFIT2.
RRID:
AB_2842158
引用格式: Affinity Biosciences Cat# DF8962, RRID:AB_2842158.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CIG-42; cig42; G10P2; GARG 39; Glucocorticoid attenuated response gene 39; IFI 54; IFI 54K; IFI-54K; IFI54; IFIT 2; IFIT-2; IFIT2; IFIT2_HUMAN; Interferon induced 54 kDa protein; Interferon induced protein 54; Interferon induced protein with tetratricopeptide repeats 2; Interferon, alpha inducible protein (MW 54kD); Interferon-induced 54 kDa protein; Interferon-induced protein with tetratricopeptide repeats 2; ISG 54 K; ISG 54K; ISG-54 K; ISG54;

抗原和靶标

免疫原:

A synthesized peptide derived from human IFIT2, corresponding to a region within the internal amino acids.

基因/基因ID:
IFIT2(3433)

文献引用

1). METTL3 promotes intrahepatic cholangiocarcinoma progression by regulating IFIT2 expression in an m6A-YTHDF2-dependent manner. Oncogene, 2022 (PubMed: 35094011) [IF=6.9]

Application: WB    Species: Human    Sample: HuCC-T1 and HCCC-9810 cells

Fig. 6 METTL3 accelerates the ICC progression by downregulating IFIT2 expression. A The mRNA level of IFIT2 after IFIT2 silencing in HuCC-T1 and HCCC-9810 cells was confirmed by RT-qPCR. B The protein level of IFIT2 after IFIT2 silencing in HuCC-T1 and HCCC-9810 cells was confirmed by western blotting. C Cell growth curve of HuCC-T1 and HCCC-9810 cells transfected with IFIT2 siRNA or Control. D Cell migration ability and cell invasion ability after IFIT2 silencing in HuCC-T1 and HCCC-9810 cells. E The mRNA level of METTL3 and IFIT2 after METTL3 or /and IFIT2 silencing in HuCC-T1 and HCCC-9810 cells was confirmed by RT-qPCR. F The protein level of METTL3 and IFIT2 after METTL3 or /and IFIT2 silencing in HuCC-T1 and HCCC-9810 cells was confirmed by western blotting. G Cell growth curve of HuCC-T1 and HCCC-9810 cells after METTL3 or /and IFIT2 silencing. H Cell migration ability and cell invasion ability in HuCC-T1 and HCCC-9810 cells after METTL3 or /and IFIT2 silencing. The results are presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, according to Student’s t test.

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