产品: HSP76 抗体
货号: DF8956
描述: Rabbit polyclonal antibody to HSP76
应用: WB
反应: Human
预测: Pig, Bovine, Horse
分子量: 71 kDa; 71kD,40kD(Calculated).
蛋白号: P17066 | P48741
RRID: AB_2842152

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(88%), Bovine(88%), Horse(83%)
克隆:
Polyclonal
特异性:
HSP76 Antibody detects endogenous levels of total HSP76.
RRID:
AB_2842152
引用格式: Affinity Biosciences Cat# DF8956, RRID:AB_2842152.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Heat shock 70 kDa protein 6; Heat shock 70 kDa protein B'; Heat shock 70 kDa protein B''; heat shock 70kD protein 6 (HSP70B'); Heat shock 70kDa protein 6; HSP70B'; HSP70B-Prime; HSP76_HUMAN; HSPA6; OTTHUMP00000032372; Heat shock 70 kDa protein B; Heat shock 70kDa protein 7 (HSP70B); HSP70B; Putative heat shock 70 kDa protein 7;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MQAPRELAVGIDLGTTYSCVGVFQQGRVEILANDQGNRTTPSYVAFTDTERLVGDAAKSQAALNPHNTVFDAKRLIGRKFADTTVQSDMKHWPFRVVSEGGKPKVRVCYRGEDKTFYPEEISSMVLSKMKETAEAYLGQPVKHAVITVPAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAAIAYGLDRRGAGERNVLIFDLGGGTFDVSVLSIDAGVFEVKATAGDTHLGGEDFDNRLVNHFMEEFRRKHGKDLSGNKRALRRLRTACERAKRTLSSSTQATLEIDSLFEGVDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDVVLVGGSTRIPKVQKLLQDFFNGKELNKSINPDEAVAYGAAVQAAVLMGDKCEKVQDLLLLDVAPLSLGLETAGGVMTTLIQRNATIPTKQTQTFTTYSDNQPGVFIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILSVTATDRSTGKANKITITNDKGRLSKEEVERMVHEAEQYKAEDEAQRDRVAAKNSLEAHVFHVKGSLQEESLRDKIPEEDRRKMQDKCREVLAWLEHNQLAEKEEYEHQKRELEQICRPIFSRLYGGPGVPGGSSCGTQARQGDPSTGPIIEEVD

MQAPRELAVGIDLGTTYSCVGVFQQGRVEILANDQGNRTTPSYVAFTDTERLVGDAAKSQAALNPHNTVFDAKRLIGRKFADTTVQSDMKHWPFQVVSEGGKPKVRVCYRGEDKTFYPEEISSMVLSKMKETAEAYLGQPVKHAVITVPTYFSNSQRQATKDAGAIAGLKVLPIINEATAAAIAYGLDRRGAGKRNVLIFDLGGGTFDVSVLSIDAGVFEVKATAGDTHLGGEDFDNRLVNHFMEEFRRKHGKDLSGNKRALRRLRTACERAKRTPSSSTQATLEIDSLFEGVDFYKSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDFVLGGGLHSHPQGAEVAAGLLQRQGAEQEHQP

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
88
Bovine
88
Horse
83
Sheep
75
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P17066/P48741 作为底物

Site PTM Type Enzyme
T39 Phosphorylation
T40 Phosphorylation
S42 Phosphorylation
Y43 Phosphorylation
T47 Phosphorylation
T49 Phosphorylation
R51 Methylation
K58 Ubiquitination
S59 Phosphorylation
K73 Ubiquitination
K79 Ubiquitination
K102 Ubiquitination
K104 Ubiquitination
K114 Ubiquitination
S123 Phosphorylation
K128 Ubiquitination
K130 Ubiquitination
T224 Phosphorylation
K259 Ubiquitination
T267 Phosphorylation
S309 Phosphorylation
S314 Phosphorylation
T315 Phosphorylation
K321 Acetylation
K321 Ubiquitination
K327 Ubiquitination
Site PTM Type Enzyme
T39 Phosphorylation
T40 Phosphorylation
S42 Phosphorylation
Y43 Phosphorylation
T47 Phosphorylation
T49 Phosphorylation
R51 Methylation
K58 Ubiquitination
S59 Phosphorylation
K73 Ubiquitination
K79 Ubiquitination
K90 Ubiquitination
K102 Ubiquitination
K104 Ubiquitination
K114 Ubiquitination
S123 Phosphorylation
K128 Ubiquitination
K130 Ubiquitination
K142 Methylation
K142 Ubiquitination
K161 Ubiquitination
Y185 Phosphorylation
T224 Phosphorylation
K259 Ubiquitination
T267 Phosphorylation
S278 Phosphorylation
S298 Phosphorylation
T300 Phosphorylation
S309 Phosphorylation
S314 Phosphorylation
T315 Phosphorylation
K321 Acetylation
K321 Ubiquitination
K327 Ubiquitination
K330 Methylation
K330 Ubiquitination
K347 Ubiquitination
K350 Ubiquitination
K359 Acetylation
K359 Sumoylation
K359 Ubiquitination
K363 Sumoylation
K363 Ubiquitination
S364 Phosphorylation
Y373 Phosphorylation
K386 Ubiquitination
S402 Phosphorylation
T407 Phosphorylation
K453 Ubiquitination
R460 Methylation
R471 Methylation
R495 Methylation
K499 Ubiquitination
K502 Acetylation
K502 Ubiquitination
T504 Phosphorylation
K509 Acetylation
K509 Sumoylation
K509 Ubiquitination
K514 Ubiquitination
K528 Ubiquitination
K541 Ubiquitination
K552 Ubiquitination
K563 Methylation
K563 Ubiquitination
K598 Ubiquitination

研究背景

功能:

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release.

蛋白家族:

The N-terminal nucleotide binding domain (NBD) (also known as the ATPase domain) is responsible for binding and hydrolyzing ATP. The C-terminal substrate-binding domain (SBD) (also known as peptide-binding domain) binds to the client/substrate proteins. The two domains are allosterically coupled so that, when ATP is bound to the NBD, the SBD binds relatively weakly to clients. When ADP is bound in the NBD, a conformational change enhances the affinity of the SBD for client proteins.

Belongs to the heat shock protein 70 family.

蛋白家族:

Belongs to the heat shock protein 70 family.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Genetic Information Processing > Transcription > Spliceosome.

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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