DNAJA1 Antibody - #DF8940

Fig. 5 IHC staining and scoring for classifying patients in Xinhua cohort based on RCC-TSC. A The flowchart of our study. Our clinical study was mainly composed of three analytic processes, including IHC staining for the tumor tissue of the 370 patients with kidney neoplasm, molecular diagnosis based on the RCC-TSC, and statistical validation. The inclusion and exclusion criteria were also shown in detail. Firstly, 370 patients with kidney neoplasm and received curative surgery from 2016–2018 were included. Secondly, due to the incompleteness of the IHC staining information (DNAJA1, DNAJB4, LAG3, Mki67), one patient was excluded. Thirdly, when performing Chi-square tests and Kaplan-Meier survival analysis, 71 (lacking OS censor and PFS censor) or 75 patients (lacking OS and PFS) were correspondingly excluded. Finally, when conducting the multivariate proportional hazards Cox regression analysis, 10 patients whose histopathological classifications were not KICH, KIRC, or KIRP were excluded. B The boxplots showed the relative expression in transcriptional level for the four markers we used for IHC staining and classification. MKi67 and LAG3, DNAJA1 and DNAJB3 respectively expressed highest in HIRC and LIRC. C The heatmap illustrated the information of the 370 patients in our retrospective cohort. D IHC slides demonstrated the differential staining results of DNAJA1, DNAJB4, LAG3, Mki67 in three RCC-TSC subtypes. Specifically, DNAJA1 and DNAJB4 both had relatively high, medium, and low staining score in LIRC, MIRC, and HIRC. While LAG3, Mki67 both had relatively high, medium, and low staining score in HIRC, MIRC, and LIRC. IHC, immunohistochemical; RCC-TSC, renal cell carcinoma-Treg states-related prognostic classification; OS, overall survival; PFS, progression-free survival; KICH, kidney chromophobe; KIRC, kidney renal clear cell carcinoma; KIRP, kidney renal papillary cell carcinoma; LIRC, low immune infiltration RCC; MIRC, medium immune infiltration RCC; HIRC, high immune infiltration RCC