CDK3 Antibody - #DF8911

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产品: CDK3 抗体
货号: DF8911
描述: Rabbit polyclonal antibody to CDK3
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse
预测: Pig, Bovine, Sheep, Rabbit
分子量: 35 kDa; 35kD(Calculated).
蛋白号: Q00526
RRID: AB_2842107

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
预测:
Pig(83%), Bovine(%), Sheep(%), Rabbit(%)
克隆:
Polyclonal
特异性:
CDK3 Antibody detects endogenous levels of total CDK3.
RRID:
AB_2842107
引用格式: Affinity Biosciences Cat# DF8911, RRID:AB_2842107.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Cdk 3; Cdk3; CDK3_HUMAN; CDKN3; Cell division kinase 3; Cell division protein kinase 3; Cyclin dependent kinase 3; Cyclin-dependent kinase 3; OTTHUMP00000206828; p36;

抗原和靶标

免疫原:
Uniprot:

Q00526(CDK3_HUMAN) >>Visit HPA database.

基因/基因ID:
CDK3(1018)
表达:
Q00526 CDK3_HUMAN:

Expressed in cancer cell lines and glioblastoma tissue.

序列:
MDMFQKVEKIGEGTYGVVYKAKNRETGQLVALKKIRLDLEMEGVPSTAIREISLLKELKHPNIVRLLDVVHNERKLYLVFEFLSQDLKKYMDSTPGSELPLHLIKSYLFQLLQGVSFCHSHRVIHRDLKPQNLLINELGAIKLADFGLARAFGVPLRTYTHEVVTLWYRAPEILLGSKFYTTAVDIWSIGCIFAEMVTRKALFPGDSEIDQLFRIFRMLGTPSEDTWPGVTQLPDYKGSFPKWTRKGLEEIVPNLEPEGRDLLMQLLQYDPSQRITAKTALAHPYFSSPEPSPAARQYVLQRFRH

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
86
Pig
83
Bovine
82
Sheep
82
Horse
75
Dog
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Serine/threonine-protein kinase that plays a critical role in the control of the eukaryotic cell cycle; involved in G0-G1 and G1-S cell cycle transitions. Interacts with CCNC/cyclin-C during interphase. Phosphorylates histone H1, ATF1, RB1 and CABLES1. ATF1 phosphorylation triggers ATF1 transactivation and transcriptional activities, and promotes cell proliferation and transformation. CDK3/cyclin-C mediated RB1 phosphorylation is required for G0-G1 transition. Promotes G1-S transition probably by contributing to the activation of E2F1, E2F2 and E2F3 in a RB1-independent manner.

组织特异性:

Expressed in cancer cell lines and glioblastoma tissue.

亚基结构:

Interacts with CABLES1 and CABLES2 (By similarity). Interacts with ATF1. Binding to CCNC/cyclin-C promotes RB1 phosphorylation.

蛋白家族:

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

文献引用

1). Overexpression of SMYD3 in Ovarian Cancer is Associated with Ovarian Cancer Proliferation and Apoptosis via Methylating H3K4 and H4K20. Journal of Cancer, 2019 (PubMed: 31417652) [IF=3.3]

Application: WB    Species: mouse    Sample: tumor

Figure 6.| Knockdown of SMYD3 inhibits ovarian cancer carcinogenesis in vivo.(F-G) The protein expression of SMYD3 and its downstream regulators CDKN3, CDKN2A, CDKN2B, CDC25A, BIRC3, CD40LG in the tumor tissues of vector control group and SMYD3 knockdown group.
2). CDK3 is a major target of miR-150 in cell proliferation and anti-cancer effect. EXPERIMENTAL AND MOLECULAR PATHOLOGY, 2017 (PubMed: 28108217) [IF=2.8]

Application: WB    Species: human    Sample: H1299 cells

Figure 6. miR-150 suppresses CDK3 expression in lung cancer compared cells. SPC1and H1299 cells were transfected with miR-150 mimics and mimics NC for forty-eight hours, respectively. A. Western blot of CDK3 in SPC1and H1299 cells. B. qRT-PCR analysis of miR-150 in SPC1and H1299 cells. C. qRT-PCR analysis of CDK3 mRNA in SPC1and H1299 cells. Data are the means ± SEM of three independent experiments. *p<0.05, **p

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