DMP1 Antibody - #DF8825

Fig. 2 SNHG1 expression in hDPSCs during the odontogenic differentiation and SNHG1 governs the odontogenic differentiation of hDPSCs. A Relative ALP, DMP-1, DSPP and SNHG1 expressions were measured by qRT-PCR at day 0, 3, and 7 under the mineralization induction condition. GAPDH was used for normalization. B The transfection efficacy of SNHG1 was measured by qRT-PCR in SNHG1 group and si-SNHG1 group, with GAPDH as a control. C The proliferation ability of hDPSCs was measured by CCK-8. D-G After SNHG1 overexpression or silence, relative ALP, DMP-1 and DSPP expression were measured by qRT-PCR and ALP, DMP-1 and DSPP expression were measured by Western blot at day 5 under the mineralization induction condition. H At 7 days after odontogenic induction, hDPSCs were performed with ALP staining and activity. I On the 14th day, Alizarin Red staining and quantification were performed. J The expression of DMP-1 and DSPP in transfected hDPSCs were also showed by Immunofluorescence Staining. Scale bar = 20 μm. *P < 0.05, **P < 0.01, ***P < 0.001

Fig. 2 SNHG1 expression in hDPSCs during the odontogenic differentiation and SNHG1 governs the odontogenic differentiation of hDPSCs. A Relative ALP, DMP-1, DSPP and SNHG1 expressions were measured by qRT-PCR at day 0, 3, and 7 under the mineralization induction condition. GAPDH was used for normalization. B The transfection efficacy of SNHG1 was measured by qRT-PCR in SNHG1 group and si-SNHG1 group, with GAPDH as a control. C The proliferation ability of hDPSCs was measured by CCK-8. D-G After SNHG1 overexpression or silence, relative ALP, DMP-1 and DSPP expression were measured by qRT-PCR and ALP, DMP-1 and DSPP expression were measured by Western blot at day 5 under the mineralization induction condition. H At 7 days after odontogenic induction, hDPSCs were performed with ALP staining and activity. I On the 14th day, Alizarin Red staining and quantification were performed. J The expression of DMP-1 and DSPP in transfected hDPSCs were also showed by Immunofluorescence Staining. Scale bar = 20 μm. *P < 0.05, **P < 0.01, ***P < 0.001

Figure 3 hSCAPs’ odontoblastic differentiation ability and FTO expression under different conditions. (a–c) ALP staining at 3 and 7 days showed decreased ALP activity, and ARS staining at day 14 indicated reduced mineralized nodules in the OM+LPS group compared to the OM group. (d) qRT-PCR revealed elevated mRNA levels of DSPP, DMP1, and COL1 in the OM group while showing decreased levels in the OM+LPS group. (e,f) Western blotting showed increased protein expression of DSPP, DMP1, and COL1 in the OM group, contrasting with decreased expression in the OM+LPS group. (g–i) qRT-PCR and Western blotting showed elevated FTO expression in OM, with reduced expression observed in OM+LPS. Control, growth medium (GM); LPS, GM with 1 µg/mL LPS; OM, odontoblastic induction medium; OM+LPS, OM with 1 µg/mL LPS; hSCAPs, human stem cells from the apical papilla; FTO, fat mass and obesity-associated protein; LPS, lipopolysaccharide; ALP, alkaline phosphatase; ARS, alizarin red S; qRT-PCR, Quantitative Real-Time Polymerase Chain Reaction; DSPP, dentin sialophosphoprotein; DMP1, dentin matrix protein-1; COL1, collagen I.

Figure 3 hSCAPs’ odontoblastic differentiation ability and FTO expression under different conditions. (a–c) ALP staining at 3 and 7 days showed decreased ALP activity, and ARS staining at day 14 indicated reduced mineralized nodules in the OM+LPS group compared to the OM group. (d) qRT-PCR revealed elevated mRNA levels of DSPP, DMP1, and COL1 in the OM group while showing decreased levels in the OM+LPS group. (e,f) Western blotting showed increased protein expression of DSPP, DMP1, and COL1 in the OM group, contrasting with decreased expression in the OM+LPS group. (g–i) qRT-PCR and Western blotting showed elevated FTO expression in OM, with reduced expression observed in OM+LPS. Control, growth medium (GM); LPS, GM with 1 µg/mL LPS; OM, odontoblastic induction medium; OM+LPS, OM with 1 µg/mL LPS; hSCAPs, human stem cells from the apical papilla; FTO, fat mass and obesity-associated protein; LPS, lipopolysaccharide; ALP, alkaline phosphatase; ARS, alizarin red S; qRT-PCR, Quantitative Real-Time Polymerase Chain Reaction; DSPP, dentin sialophosphoprotein; DMP1, dentin matrix protein-1; COL1, collagen I.

Figure 2. |LncRNA CALB2 promotes odontoblast differentiation of hDPSCs.(E) Protein levels of DSPP and DMP-1 were detected by western blot in indicated groups.

Figure 1 Expression of DSPP, nestin, DMP1, Runx2, and periostin by dentin induction of dental pulp stem cells. Immunohistochemical staining images after the start of dentin induction 21 days. (Bule: Hoechst 33342 and Green: (a) DSPP, (b) nestin, (c) DMP1, (d) Runx2, and (e) periostin).