PPP2R1A Antibody - #DF8794

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产品: PPP2R1A 抗体
货号: DF8794
描述: Rabbit polyclonal antibody to PPP2R1A
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Xenopus
分子量: 65 kDa; 65kD(Calculated).
蛋白号: P30153
RRID: AB_2841992

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
PPP2R1A Antibody detects endogenous levels of total PPP2R1A.
RRID:
AB_2841992
引用格式: Affinity Biosciences Cat# DF8794, RRID:AB_2841992.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

2AAA_HUMAN; 6330556D22Rik; Alpha isoform of regulatory subunit A, protein phosphatase; DKFZp470L0914; Medium tumor antigen-associated 61 kDa protein; MGC128399; MGC786; MGC83000; MGC95083; PP2A Aalpha; PP2A; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; PP2A, subunit A, PR65-alpha isoform; PP2A, subunit A, R1-alpha isoform; PP2AAALPHA; Ppp2r1a; ppp2r1a-b; PR65; PR65-alpha; PR65A; Protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; Protein phosphatase 2 (Formerly 2A), regulatory subunit A (PR 65), alpha isoform, isoform CRA_a; Protein phosphatase 2 (formerly 2A), regulatory subunit A,; Protein phosphatase 2 regulatory subunit A alpha; Protein phosphatase 2, 65-KD regulatory subunit A, alpha; Protein phosphatase 2, regulatory subunit A, alpha isoform; Protein phosphatase 2, structural/regulatory subunit A, alpha; PPP2R1A; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform;

抗原和靶标

免疫原:
Uniprot:

P30153(2AAA_HUMAN) >>Visit HPA database.

基因/基因ID:
PPP2R1A(5518)
序列:
MAAADGDDSLYPIAVLIDELRNEDVQLRLNSIKKLSTIALALGVERTRSELLPFLTDTIYDEDEVLLALAEQLGTFTTLVGGPEYVHCLLPPLESLATVEETVVRDKAVESLRAISHEHSPSDLEAHFVPLVKRLAGGDWFTSRTSACGLFSVCYPRVSSAVKAELRQYFRNLCSDDTPMVRRAAASKLGEFAKVLELDNVKSEIIPMFSNLASDEQDSVRLLAVEACVNIAQLLPQEDLEALVMPTLRQAAEDKSWRVRYMVADKFTELQKAVGPEITKTDLVPAFQNLMKDCEAEVRAAASHKVKEFCENLSADCRENVIMSQILPCIKELVSDANQHVKSALASVIMGLSPILGKDNTIEHLLPLFLAQLKDECPEVRLNIISNLDCVNEVIGIRQLSQSLLPAIVELAEDAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKFGKEWAHATIIPKVLAMSGDPNYLHRMTTLFCINVLSEVCGQDITTKHMLPTVLRMAGDPVANVRFNVAKSLQKIGPILDNSTLQSEVKPILEKLTQDQDVDVKYFAQEALTVLSLA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
91
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P30153 作为底物

Site PTM Type Enzyme
A2 Acetylation
S9 Phosphorylation
K34 Ubiquitination
S36 Phosphorylation
T37 Phosphorylation
K107 Ubiquitination
S120 Phosphorylation
S122 Phosphorylation
K133 Ubiquitination
R134 Methylation
K163 Ubiquitination
R167 Methylation
K188 Acetylation
K188 Ubiquitination
K194 Ubiquitination
K202 Ubiquitination
K255 Ubiquitination
K266 Ubiquitination
K272 Ubiquitination
K280 Acetylation
K280 Ubiquitination
K292 Ubiquitination
K305 Ubiquitination
K307 Ubiquitination
S335 Phosphorylation
K342 Ubiquitination
S343 Phosphorylation
S353 Phosphorylation
K374 Ubiquitination
C390 S-Nitrosylation
K416 Ubiquitination
K442 Sumoylation
K467 Ubiquitination
K472 Ubiquitination
K475 Ubiquitination
K485 Ubiquitination
Y495 Phosphorylation
K519 Ubiquitination
R527 Methylation
K542 Ubiquitination
K546 Ubiquitination
S554 Phosphorylation
K561 Acetylation
K561 Ubiquitination
K566 Ubiquitination
T584 Phosphorylation

研究背景

功能:

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis.

细胞定位:

Cytoplasm. Nucleus. Chromosome>Centromere. Lateral cell membrane. Cell projection>Dendrite.
Note: Centromeric localization requires the presence of BUB1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD (By similarity). Interacts with FOXO1; the interaction dephosphorylates FOXO1 on AKT-mediated phosphorylation sites (By similarity). PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGO1. Interacts with PLA2G16; this interaction might decrease PP2A activity. Interacts with CTTNBP2NL. Interacts with GNA12; the interaction promotes protein phosphatase 2A activation causing dephosphorylation of MAPT. Interacts with CIP2A; this interaction stabilizes CIP2A. Interacts with FAM122A. Interacts with ADCY8; antagonizes interaction between ADCY8 and calmodulin (By similarity). Interacts with CRTC3 (when phosphorylated at 'Ser-391').

蛋白家族:

Each HEAT repeat appears to consist of two alpha helices joined by a hydrophilic region, the intrarepeat loop. The repeat units may be arranged laterally to form a rod-like structure.

Belongs to the phosphatase 2A regulatory subunit A family.

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Parasitic > Chagas disease (American trypanosomiasis).

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Nervous system > Dopaminergic synapse.

· Organismal Systems > Nervous system > Long-term depression.

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