产品: 磷酸化 RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) 抗体
货号: DF8743
描述: Rabbit polyclonal antibody to Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232)
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: Q15418 | P51812 | Q15349 | Q9UK32
RRID: AB_2841947

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   规格 价格 库存
 100ul RMB¥ 2400 现货
 200ul RMB¥ 3200 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-RSK1/2/3/4 (Ser221/Ser227/Ser218/Ser232) Antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at Ser221/227/S218/232.
RRID:
AB_2841947
引用格式: Affinity Biosciences Cat# DF8743, RRID:AB_2841947.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

90 kDa ribosomal protein S6 kinase 1; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1; dJ590P13.1; EC 2.7.11.1; HU 1; HU1; KS6A1_HUMAN; MAP kinase activated protein kinase 1a; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPK1A; MGC79981; Mitogen-activated protein kinase-activated protein kinase 1A; OTTHUMP00000004113; p90 RSK1; p90-RSK 1; p90rsk; p90RSK1; p90S6K; pp90RSK1; Ribosomal protein S6 kinase 90kD 1; Ribosomal protein S6 kinase 90kD polypeptide 1; Ribosomal protein S6 kinase 90kDa polypeptide 1; Ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase polypeptide 1; Ribosomal S6 kinase 1; RPS6K1 alpha; rps6ka; Rps6ka1; RSK 1; RSK 1 p90; RSK; RSK-1; RSK1; S6K alpha 1; S6K-alpha-1; 90 kDa ribosomal protein S6 kinase 3; CLS; HU 3; HU2; HU3; Insulin stimulated protein kinase 1; Insulin-stimulated protein kinase 1; ISPK-1; ISPK1; KS6A3_HUMAN; MAP kinase activated protein kinase 1b; MAP kinase-activated protein kinase 1b; MAPK activated protein kinase 1b; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK 1b; MAPKAPK-1b; MAPKAPK1B; Mental retardation, X linked 19; MRX19; OTTHUMP00000023036; p90 RSK2; p90 RSK3; p90-RSK 3; p90RSK3; pp90RSK2; Ribosomal protein S6 kinase 90kDa polypeptide 3; Ribosomal protein S6 kinase alpha 3; Ribosomal protein S6 kinase alpha-3; Ribosomal protein s6 kinase ii alpha 2; Ribosomal S6 kinase 2; Rps6ka3; RSK; RSK-2; RSK2; S6 kinase 2; S6K alpha3; S6K-alpha-3; 90 kDa ribosomal protein S6 kinase 2; HU 2; KS6A2_HUMAN; MAP kinase activated protein kinase 1c; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; MAPKAPK1C; Mitogen.activated protein kinase-activated protein kinase 1C; p90 RSK3; p90-RSK 2; p90RSK2; pp90RSK3; Ribosomal protein S6 kinase alpha-2; ribosomal protein S6 kinase, 90kDa, polypeptide 2; Ribosomal S6 kinase 3; RPS6KA2; RSK 3; RSK; RSK-3; S6K alpha; S6K alpha 2; S6K-alpha-2; 90 kDa ribosomal protein S6 kinase 6; KS6A6_HUMAN; p90 RSK 6; p90-RSK 6; p90RSK6; pp90RSK4; Ribosomal protein S6 kinase 90kDa polypeptide 6; Ribosomal protein S6 kinase alpha 6; Ribosomal protein S6 kinase alpha-6; Ribosomal S6 kinase 4; rps6ka6; RSK 4; RSK-4; S6K-alpha-6;

抗原和靶标

免疫原:

A synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/S218/232.

基因/基因ID:

研究领域

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Nervous system > Long-term potentiation.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

文献引用

1). Activation of RSK2 upregulates SOX8 to promote methotrexate resistance in gestational trophoblastic neoplasia. LABORATORY INVESTIGATION, 2021 (PubMed: 34373588) [IF=5.1]

Application: IHC    Species: Human    Sample: GTN

Fig. 6RSK2/SOX8 signaling was highly enriched in MTX-resistant GTN samples. A Immunostaining of p-RSK and SOX8 in GTN samples. Representative IHC staining micrographs were shown for MTX-resistant (left panel) or MTX-sensitive (right panel) GTNs. Bars: 100 µm. B p-RSK and SOX8 were highly expressed in MTX-resistant GTN specimens. *P < 0.05; **P < 0.01. C High expression of p-RSK was significantly correlated with high expression of SOX8 in GTN specimens.

Application: WB    Species: Human    Sample: JAR/MTX and JAR cell

Fig. 1Quantitative phosphoproteomic analysis on JAR/MTX and JAR cell models. A Experimental scheme for the quantitative phosphoproteomic analysis on JAR and JAR/MTX cell models. B Differentially expressed phosphosites and phosphoproteins identified by TMT labeling, IMAC enrichment of phosphopeptides, and LC-MS/MS in JAR and JAR/MTX cell models. C Altered expression of phosphosites in protein kinases and phosphatases in JAR/MTX cells. D Western blotting analysis on p-RSK expression in MTX-resistant GTN sublines.

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