Phospho-EGFR (Tyr1068)[Tyr1092] Antibody - #AF3045

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产品: 磷酸化 EGFR (Tyr1068)[Tyr1092] 抗体
货号: AF3045
描述: Rabbit polyclonal antibody to Phospho-EGFR (Tyr1068)[Tyr1092]
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit, Xenopus
蛋白号: P00533
RRID: AB_2834472

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-EGFR (Tyr1068) Antibody detects endogenous levels of EGFR only when phosphorylated at Tyr1092, which site historically referenced as Tyr1068.
RRID:
AB_2834472
引用格式: Affinity Biosciences Cat# AF3045, RRID:AB_2834472.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Avian erythroblastic leukemia viral (v erb b) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; EGFR_HUMAN; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog); Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian); Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; ERBB; ERBB1; Errp; HER1; mENA; NISBD2; Oncogen ERBB; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine-protein kinase ErbB-1; SA7; Species antigen 7; Urogastrone; v-erb-b Avian erythroblastic leukemia viral oncogen homolog; wa2; Wa5;

抗原和靶标

免疫原:

A synthesized peptide derived from human EGFR around the phosphorylation site of Tyr1097.

基因/基因ID:
EGFR(1956)
描述:
EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). Dual-responsive nanoparticles loading bevacizumab and gefitinib for molecular targeted therapy against non-small cell lung cancer. Acta Pharmacologica Sinica, 2022 (PubMed: 35705687) [IF=6.9]

Application: WB    Species: Human    Sample: HCC827 cells

Fig. 2 BCGN induced in vitro inhibition of p-EGFR. a Uptake of BCGN when HCC827 cells were treated with BCGN for different time periods and examined by PPa fluorescence with flow cytometry. b CLSM images of HCC827 cells with BCGN treatments. Cell nuclei were labeled with DAPI (blue). membrane was labeled with WGA-FITC (green) and PPa was represented in red. Scale bars, 25 μm. c Cytotoxicity of gefitinib, IgG-NP, GN, BCGN after incubation with HCC827 tumor cells for 48 h (n = 5). IgG-NP were human IgG coated nanoparticles without loading gefitinib. d Immunofluorescence analysis of p-EGFR levels in HCC827 cells after treated by BCGN or gefitinib for 6 h. Cytoskeleton was labeled with Phalloidin-TRITC (red) and p-EGFR was stained with FITC linked antibodies (green). Scale bars, 20 μm. e Western blotting detecting the efficiency of BCGN for inhibiting phosphorylation of EGFR, AKT and ERK in HCC827 cells. Data are shown as mean ± SD.
2). Discovery of Novel Small-Molecule-Based Potential PD-L1/EGFR Dual Inhibitors with High Druggability for Glioblastoma Immunotherapy. Journal of medicinal chemistry, 2024 (PubMed: 38739112) [IF=6.8]
3). MCP-enhanced SOD3 activity inhibits gastric cancer and potentiate chemotherapy via modulating EGFR signaling. Life sciences, 2024 (PubMed: 39746602) [IF=6.1]
4). TIAM2 Contributes to Osimertinib Resistance, Cell Motility, and Tumor-Associated Macrophage M2-like Polarization in Lung Adenocarcinoma. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022 (PubMed: 36142328) [IF=5.6]
5). Ethyl acetate extract of Ruta graveolens: a specific and potent inhibitor against the drug-resistant EGFR_T790M mutant in NSCLC. Frontiers in pharmacology, 2025 (PubMed: 40365309) [IF=5.6]

Application: WB    Species: human    Sample: H1975 cells, A431 cells, A549 cells, H1299 cells

Figure 10. Immunoblot analysis of EGFR phosphorylation inhibition in various cell lines treated with EAE. Panel shows: (A) H1975 cells, (B) A431 cells, (C) A549 cells, (D) H1299 cells. Cells were untreated or treated with DMSO (vehicle control) or EAE (100 and 200 μg) for 48 h. Cell lysates were analyzed for EGFR, phospho-EGFR (Tyr1068/1092 and Tyr1173/1197), and GAPDH (loading control) by immunoblotting. The experiment was performed in triplicates, so the densitometry analysis, represented as bar graphs with error bars, showed significant inhibition of EGFR phosphorylation in all cell lines following EAE treatment compared to control expression of EGFR across all cell lines. (n = 3).
6). Low-intensity ultrasound combined with arsenic trioxide induced apoptosis of glioma via EGFR/AKT/mTOR. Life Sciences, 2023 (PubMed: 37730111) [IF=5.2]

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