Phospho-EGFR (Ser1070) Antibody - #AF3044

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产品: 磷酸化 EGFR (Ser1070) 抗体
货号: AF3044
描述: Rabbit polyclonal antibody to Phospho-EGFR (Ser1070)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Sheep, Rabbit
蛋白号: P00533
RRID: AB_2834471

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-EGFR (Ser1070) Antibody detects endogenous levels of EGFR only when phosphorylated at Serine 1070.
RRID:
AB_2834471
引用格式: Affinity Biosciences Cat# AF3044, RRID:AB_2834471.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Avian erythroblastic leukemia viral (v erb b) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; EGFR_HUMAN; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog); Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian); Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; ERBB; ERBB1; Errp; HER1; mENA; NISBD2; Oncogen ERBB; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine-protein kinase ErbB-1; SA7; Species antigen 7; Urogastrone; v-erb-b Avian erythroblastic leukemia viral oncogen homolog; wa2; Wa5;

抗原和靶标

免疫原:

A synthesized peptide derived from human EGFR around the phosphorylation site of Ser1070.

基因/基因ID:
EGFR(1956)
描述:
EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Endometrial cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Glioma.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Melanoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Non-small cell lung cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). EFEMP2 upregulates PD-L1 expression via EGFR/ERK1/2/c-Jun signaling to promote the invasion of ovarian cancer cells. Cellular & molecular biology letters, 2023 (PubMed: 37420173) [IF=9.2]

Application: WB    Species: Mouse    Sample:

Fig. 5 Confirmation of EFEMP2-EGFR binding and the effect of down or up expression of EFEMP2 on ERK1/2/c-Jun pathway or the JNK/c-Jun pathway. a By co-IP assay, the EGF-related protein EFEMP2 was confirmed to bind to EGFR. b By western blot, EFEMP2 knockdown significantly reduced the expression and phosphorylation level of EGFR, ERK1/2 and c-Jun, however, the expression and phosphorylation level of JNK remained unchanged. c Conversely, EFEMP2 overexpression increased EGFR, ERK1/2 and c-Jun expression and their phosphorylation levels, but had no effect on the expression and phosphorylation level of JNK. Western blot was used to analyze the expression and phosphorylation level of ERK1/2/c-Jun pathway and PD-L1 expression in (d) ES2 and (e) OVCAR-3 EX after trametinib (0 μmol/l, 4 μmol/l, 8 μmol/l, and 12 μmol/l) treatment. The transwell chamber was used to detect the migration and invasion ability of (f) ES-2 and (g) OVCAR-3 EX cells after trametinib (0 μmol/l, 4 μmol/l, 8 μmol/l, and 12 μmol/l) treatment. Magnification × 200
2). Higenamine alleviates allergic rhinitis by activating AKT1 and suppressing the EGFR/JAK2/c-JUN signaling. PHYTOMEDICINE, 2021 (PubMed: 33945919) [IF=6.7]

Application: WB    Species: Human    Sample: human nasal epithelial cell

Fig. 8. The effects of higenamine (HG) on potential targets in histamine-induced HNEpCs. (A-J) Levels of protein expression of AKT1, p-AKT1, EGFR, p-EGFR, c-Jun, p-c-Jun, iNOS, JAK2, and p-JAK2 were determined by Western blotting, β-actin was used as an internal control. Data are expressed as means ± SD of three experiments. ## p < 0.01, and # p < 0.05 compared with the control group. ** p < 0.01, and * p < 0.05 compared with the histamine group.
3). Processed product (Pinelliae Rhizoma Praeparatum) of Pinellia ternata (Thunb.) Breit. Alleviates the allergic airway inflammation of cold phlegm via regulation of PKC/EGFR/MAPK/PI3K-AKT signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY, 2022 (PubMed: 35688257) [IF=4.8]
4). Beneficial effects of mijianchangpu decoction on ischemic stroke through components accessing to the brain based on network pharmacology. JOURNAL OF ETHNOPHARMACOLOGY, 2022 (PubMed: 34848358) [IF=4.8]
5). miR-370-3p Alleviates Ulcerative Colitis-Related Colorectal Cancer in Mice Through Inhibiting the Inflammatory Response and Epithelial-Mesenchymal Transition. Drug Design Development and Therapy, 2020 (PubMed: 32214798) [IF=4.7]

Application: WB    Species: mouse    Sample: tumor colon

Figure 3 |Effect of miR-370-3p on inflammatory response and TLR4 signaling pathway in AOM/DSS-induced mice.(D) The expression levels of TLR4, COX-2, and pEGFR in the tumor colon tissues from each group were determined by Western blot, and the relative band intensity was analyzed.The comparison among groups was performed by One-way ANOVA. Data are presented as mean ± SD. ##p<0.01 compared with the Sham group, **p<0.01 compared with the Ad-control group.
6). The increased expression of cytokeratin 13 leads to an increase in radiosensitivity of nasopharyngeal carcinoma HNE-3 cells by upregulating ERRFI1. IUBMB life, 2023 (PubMed: 37070291) [IF=3.7]

Application: WB    Species: human    Sample: HNE-3 cells

FIGURE 3 CK13 exerts anticancer effects by upregulating ERRFI1. (a and b) Western blotting (WB) and qPCR were used to measure ERRFI1 expression after transient transfection of CK13 overexpression plasmid or control plasmid. **p 
7). Epidermal growth factor receptor as a potential target of momordin Ic to promote apoptosis of cholangiocarcinoma cells Get access Arrow. JOURNAL OF PHARMACY AND PHARMACOLOGY, 2022 (PubMed: 35640567) [IF=2.8]
8). Louqin Zhisou Decoction Inhibits Mucus Hypersecretion for Acute Exacerbation of Chronic Obstructive Pulmonary Disease Rats by Suppressing EGFR-PI3K-AKT Signaling Pathway and Restoring Th17/Treg Balance. Evidence-based Complementary and Alternative Medicine, 2019 (PubMed: 30800170)

Application: WB    Species: rat    Sample: lung

Figure 4:| Efect of LQZS on phosphorylation levels of EGFR, PI3K, and AKT in lung tissues of rats with AECOPD. (a) Quantitative detection of ratios of phospho-EGFR to total EGFR.

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