NOX1 Antibody - #DF8684

Fig. 1 Oxidative damage as observed in the hippocampal DG region and vmPFC of CUS rats. A Behavioral responses of FST and SPT in control rats and CUS model rats (N = 50 per group). B Activity of antioxidant enzymes SOD and T-AOC. Contents of MDA and LDH were analyzed and levels were normalized to total protein content (N = 5–6 per group). C Representative western blot images showing relative protein levels NOX1 and NOX4 (N = 6 per group). D Q-PCR analysis of NOX1 and NOX4 mRNA levels of each group. Band intensities were normalized to GAPDH (N = 6 per group). E Representative images of DHE staining (red) within the DG area from each group of rats following ROS analysis (N = 6 per group). Nuclei (blue) are stained with DAPI. Scale bar is 50 μm. F ROS relative intensity analysis after DHE staining in each group *P 

Figure 5 Effects of Y-1 and Y-2 on oxidative stress of NRK-52e cells stimulated by sodium oleate with/without CD36 silences. The expression of NOX1, Nrf2, and Keap1 protein in NRK-52e cells was quantified by Western blot and normalized (n = 3) (A). The levels of SOD and MDA in NRK-52e cells were determined by colorimetric method (n = 6) (B). The levels of ROS in NRK-52e cells were determined by FlowSight multi-dimensional panoramic flow cytometer (n = 3) (C). Compared with NC or NC-sicD36 group, ## p < 0.01; compared with M or M-sicD36 group, * p < 0.05, ** p < 0.01.

Supplementary Figure S4|A,BNADPH oxidases of Nox family consisted of seven Nox homologues and are the prominent source of ROS generation. Nox1, Nox2, and Nox4 were found expressed in kidneys. Our results showed that neither Nox1 nor Nox2 expression levels were affected by H/R injury.

Figure 5 Luteolin inhibits ferroptosis in rat gastric tissue. (A) Changes in the levels of Fe2+ and MDA in gastric tissues of luteolin-treated rats. (B) Changes in the mRNA expression of FIH1, COX2, ACSL4, GPX4, and NOX1 were detected using qPCR. (C) Western blot analysis of the protein expression levels of FIH1, COX2, ACSL4, GPX4, and NOX1.

Figure 6 NGR1 induces ferroptosis in breast cancer cells by down-regulating RUNX2. (A) Changes in the concentration of MDA and Fe2+ in MDA-MB-23 and SK-BR-3 cells were detected after NGR1 treatment. (B, C) Changes in the expression of ACSL4, COX2, FIH1, GPX4, and NOX1 proteins were detected by Western blot in MDA-MB-231 and SK-BR-3 cells. (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).