NFAT3 Antibody - #DF8682

Figure 4 HIPK1 knockdown inhibits pathological hypertrophy of cardiomyocytes through inactivation of the CREB-C/EBPβ axis. a) qRT-PCR for CREB in NRCMs transfected with OE-CREB plasmid or control vector (n = 6). b) Immunofluorescent staining for α-actinin in NRCMs treated with sh-HIPK1 lentivirus and/or OE-CREB plasmid (n = 6). Scale bar = 100 µm. c) qRT-PCR for C/EBPβ in NRCMs transfected with OE-C/EBPβ plasmid or control vector (n = 5). d) Immunofluorescent staining for α-actinin in NRCMs treated with sh-HIPK1 lentivirus and/or OE-C/EBPβ plasmid (n = 6). Scale bar = 100 µm. e) H9C2 cell lysates were incubated with C/EBPβ antibody, and co-immunoprecipitation of C/EBPβ with NFAT3 and GATA4 was evaluated by Western blot. f) qRT-PCR for genes associated with pathological cardiac hypertrophy in NRCMs (n = 6). g,h) Western blot for CREB phosphorylation and C/EBPβ expressions in heart tissues from WT and HIPK1 KO mice with g) sham or h) TAC operations (n = 3). i,j) qRT-PCR for C/EBPβ expression in heart tissues from WT and HIPK1 KO mice with i) sham or j) TAC operations (n = 5–6). Data between two groups were compared by independent-sample two-tailed Student's t-test. Data among four groups were compared by two-way ANOVA test followed by Tukey post hoc test. *, p < 0.05; **, p < 0.01; ***, p < 0.001.