产品: KIF20A 抗体
货号: DF8671
描述: Rabbit polyclonal antibody to KIF20A
应用: WB IHC IF/ICC
文献验证: WB
反应: Human
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: O95235
RRID: AB_2841875

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
KIF20A Antibody detects endogenous levels of total KIF20A.
RRID:
AB_2841875
引用格式: Affinity Biosciences Cat# DF8671, RRID:AB_2841875.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AA415432; FLJ21151; GG10 2; GG10_2; KI20A_HUMAN; KIF20A; kinesin family member 20A; Kinesin like protein KIF20A; Kinesin-like protein 174; Kinesin-like protein KIF20A; mitotic kinesin like protein 2; Mitotic kinesin-like protein 2; MKlp2; OTTHUMP00000159481; RAB6 interacting, kinesin like (rabkinesin6); Rab6-interacting kinesin-like protein; RAB6-INTERACTING PROTEIN, KINESIN-LIKE; RAB6KIFL; Rabkinesin 6; Rabkinesin-6;

抗原和靶标

免疫原:

A synthesized peptide derived from human KIF20A, corresponding to a region within C-terminal amino acids.

基因/基因ID:

文献引用

1). Long noncoding RNA SH3PXD2A-AS1 promotes NSCLC proliferation and accelerates cell cycle progression by interacting with DHX9. Cell death discovery, 2022 (PubMed: 35410446) [IF=7.0]

Application: WB    Species: human    Sample: A549, H1299, H292 and H23 cells

Fig. 3: Identification of cytokine-related genes as probable target genes of SH3PXD2A-AS1 in NSCLC proliferation. A Cluster analysis of expression patterns of genes/transcripts in the selected gene set. The colour in the figure represents the normalised expression value of the gene in each sample. Red represents higher expression of the gene in the sample, and blue represents lower expression. B GO and KEGG enrichment analyses were used to classify the significantly differentially expressed genes of cancer cells. C The significantly differentially expressed genes of H292 cells with SH3PXD2A-AS1 knockdown in sequencing results were verified by qRT-PCR assays. D, E Effect of SH3PXD2A-AS1 OE or KD on the protein expression of FOXM1, KIF20A and CENPF in A549, H1299, H292 and H23 cells, as assessed by Western blottings. Tubulin was used as a reference control. F, G Relative mRNA expression levels of SH3PXD2A-AS1, FOXM1, KIF20A and CENPF in A549, H1299, H292 and H23 cells with SH3PXD2A-AS1 OE or KD. The 18 S gene was used as a reference control. Lnc2(SH3PXD2A-AS1). Data are shown as the mean ± standard deviation from three independent experiments. *P 

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