COPS3 Antibody - #AF0187

(3)  
产品: COPS3 抗体
货号: AF0187
描述: Rabbit polyclonal antibody to COPS3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 47kDa; 48kD(Calculated).
蛋白号: Q9UNS2
RRID: AB_2833380

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IF/ICC: 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)
克隆:
Polyclonal
特异性:
COPS3 Antibody detects endogenous levels of total COPS3.
RRID:
AB_2833380
引用格式: Affinity Biosciences Cat# AF0187, RRID:AB_2833380.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Constitutive photomorphogenic homolog subunit 3; COP 9 (constitutive photomorphogenic) subunit 3; COP 9 complex homolog subunit 3; COP 9 complex S3; COP 9 complex subunit 3; COP 9 constitutive photomorphogenic homolog subunit 3; COP 9 homolog; COP 9 signalosome complex subunit 3; COP 9 signalosome subunit 3; COP 9 subunit 3; COP9 (constitutive photomorphogenic) subunit 3; COP9 complex homolog subunit 3; COP9 complex S3; COP9 complex subunit 3; COP9 constitutive photomorphogenic homolog subunit 3; COP9 homolog; COP9 signalosome complex subunit 3; COP9 signalosome subunit 3; COP9 subunit 3; COPS 3; cops3; CSN 3; CSN3; CSN3_HUMAN; JAB 1 containing signalosome subunit 3; JAB1 containing signalosome subunit 3; JAB1-containing signalosome subunit 3; SGN 3; SGN3; Signalosome subunit 3;

抗原和靶标

免疫原:
Uniprot:

Q9UNS2(CSN3_HUMAN) >>Visit HPA database.

基因/基因ID:
COPS3(8533)
表达:
Q9UNS2 CSN3_HUMAN:

Widely expressed. Expressed at high level in heart and skeletal muscle.

描述:
COPS3 Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases.
序列:
MASALEQFVNSVRQLSAQGQMTQLCELINKSGELLAKNLSHLDTVLGALDVQEHSLGVLAVLFVKFSMPSVPDFETLFSQVQLFISTCNGEHIRYATDTFAGLCHQLTNALVERKQPLRGIGILKQAIDKMQMNTNQLTSIHADLCQLCLLAKCFKPALPYLDVDMMDICKENGAYDAKHFLCYYYYGGMIYTGLKNFERALYFYEQAITTPAMAVSHIMLESYKKYILVSLILLGKVQQLPKYTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVNKHSETFTRDNNMGLVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFASINQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEITVNPQFVQKSMGSQEDDSGNKPSSYS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
92
Zebrafish
69
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UNS2 作为底物

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
K30 Ubiquitination
K37 Ubiquitination
K125 Ubiquitination
K156 Ubiquitination
K171 Ubiquitination
Y227 Phosphorylation
K243 Ubiquitination
K254 Ubiquitination
S273 Phosphorylation
K281 Ubiquitination
K296 Ubiquitination
K304 Ubiquitination
K305 Ubiquitination
K312 Ubiquitination
S318 Phosphorylation
S324 Phosphorylation
S350 Phosphorylation
K366 Ubiquitination
Y367 Phosphorylation
K382 Ubiquitination
K391 Ubiquitination
K406 Ubiquitination
S407 Phosphorylation
S410 Phosphorylation
S415 Phosphorylation
K418 Ubiquitination
S421 Phosphorylation
Y422 Phosphorylation
S423 Phosphorylation

研究背景

功能:

Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.

细胞定位:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Expressed at high level in heart and skeletal muscle.

亚基结构:

Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COPS6, COPS7 (COPS7A or COPS7B), COPS8 and COPS9 isoform 1. In the complex, it probably interacts directly with COPS1, COPS4, COPS8 and COPS9 isoform 1. Interacts with CK2 and PKD. Interacts with the translation initiation factor EIF3S6 and IKBKG. Interacts with ERCC6.

蛋白家族:

Belongs to the CSN3 family.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.