Synphilin-1 Antibody - #DF8619

Figure 7 Sphingolipids regulate SY1-induced cytotoxicity and SY1 IB morphology in mammalian cells. (A) Inhibition of the intracellular sphingolipid synthesis pathway by myriocin enhanced SY1 cytotoxicity based on mitochondrial membrane potential and ATP content assays. Data were analyzed using the Student’s t-test. Error bars indicate the SD, n=5-9 biological replicates. (B) Addition of exogenous DHS rescued the cytotoxicity caused by SY1 expression. Data were analyzed using the Student’s t-test. Error bars indicate the SD, n=4-9 biological replicates. (C) Exogenous addition of DHS to SPTLC2 knockout cells restored the typical spherical SY1 IBs in HEK293t. Statistics of the ratio of cells with dispersed irregular IBs after exogenous addition of DMSO and DHS to HEK293t SPTLC2 knockout cells (See also Figure 7—figure supplement 2). Scale bar = 20 μm. Data were analyzed using the Student’s t-test. Error bars indicate the SD, n=5 biological replicates. (D) DHS ameliorated SY1 cytotoxicity by normalizing mitochondrial membrane potential and cell viability. Data were analyzed using the Student’s t-test. Error bars indicate the SD, n=6 biological replicates.