OPA1 Antibody - #DF8587

Figure 6. Selene was specifically enriched in the mitochondria through TLR4/TRAF3/MFN1 protein complex-mediated membrane fusion. A. Results of potential bridge-linked protein analysis from FpClass database. B. Pull-down assay results (IP by the anti-Flag beads) of the input, control vector, and Flag-TLR4 overexpression groups detected via silver staining to identify proteins interacting with TLR4. C-E. Duolink PLA results of TLR4/TRAF3 and TRAF3/MFN1 after Selene treatment (Selene-L 10 µM, Selene-H 15 µM). F. Whole-cell lysates from OVCAR-3 were immunoprecipitated by anti-TRAF3, anti-TLR4, anti-MFN1, and anti-OPA1 antibodies, followed by Western blot with antibodies against the indicated proteins to verify the interactions of proteins. G. Cell lysate was separated by FPLC. Then, TRAF3, TLR4, and MFN1 in the fractions were detected using Western blot. Results are shown as means ± SD (*P < 0.05, **P < 0.01).

Fig. 1.| Cr(VI) induced mitochondrial fission in the hepatocytes. (C) The protein expressions of the five molecules control the fusion/fission process, OPA1, MFN1, MFN2, FIS1, and Drp1 were assayed using western blotting. Mitochondrial protein was also extracted to determine the protein level of Drp1. VDAC1 (32 kDa) was the internal standard for the mitochondrial protein.

Figure 7 Mitochondrial dynamics participate in osteogenesis mediated by FGFR/FGFR2-AMPK pathway: (A) Mito-tracker red and mitochondria 2D analysis showed more fragmented or punctate mitochondria with fewer and shorter branches in the MT group, while there were widely reticular mitochondria with longer branches in the WT group. (B) qRT-PCR and Western blot analysis demonstrated that the expressions of mitochondrial-fusion-related factors Mfn2 and Opa1 were downregulated, however, the expression of mitochondrial-fission-related factor Drp1 was upregulated in the MT group compared to the WT group. (C) Mito-tracker red and mitochondria 2D analysis showed a filamentous network of mitochondria with more and longer branches widely spread in the cytoplasm in the siFGFR2 group. (D) qPCR and Western blot analysis showed that the expression of fusion-related genes Mfn2 and Opa1 was increased and the expression of fission-related gene Drp1 was decreased in the siFGFR2 group. (E) After treatment with Compound C, the morphology of the mitochondria which should have tended to split adopted a fused reticular structure with more and longer branches, as shown by Mito-tracker red and mitochondria 2D analysis. (F) Western blot analysis showed the level of MFN2 and OPA1 was increased and then level of DRP1 was decreased, as induced by Compound C. p values were significant at * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.

Fig. 3: PICK1 overexpression regulates mitochondrial quality control in endotoxin- induced acute lung injury in mice.