SLC22A6 Antibody | Affinity Biosciences LTD|亲科生物官网

WB
IF/ICC
Cites

1/5

SLC22A6 Antibody - Western blot analysis of extracts from HepG2 cells(LPS 4h treatment), using SLC22A6 Antibody.

SLC22A6 Antibody - DF8582 staining Hela cells by IF/ICC.

$FIGURE 4 DTX treatment decreased the expression of GLUT9, OAT1, and OAT3 in the HUA quail kidneys (n = 3). The melting curves of RT-PCR products of renal (A) GLUT9, (B) OAT3, (C) GAPDH, (D) OAT1, and (E) β-actin in the quail kidneys. The amplification curve of the RT-PCR products of (F) GLUT9, (G) OAT3, (H) GAPDH, (I) OAT1, and (J) β-actin in the quail kidneys. The bar diagram showing the relative expression levels of (K) GLUT9 mRNA, (L) OAT3 mRNA, and (M) OAT1 mRNA. (N) Results of Western blotting showing the relative expression levels of GLUT9, OAT1, OAT3, and GAPDH in the kidneys of control, HUA model, and drug-treated quails. An equal amount (75 µg/lane) of total protein was loaded in each lane of 10% SDS-polyacrylamide gel, fractionated, and then analyzed. The bar diagram shows the relative expression levels of the (O) GLUT9 protein, (P) OAT1 protein, and (Q) OAT3 protein in the quail kidneys. Relative expression was estimated after normalizing with GAPDH/β-actin. #p < 0.05 and ##p < 0.01 compared with the control group. *p < 0.05 and **p < 0.01 compared with the model group; ns: p > 0.05.$

SLC22A6 Antibody - FIGURE 4 DTX treatment decreased the expression of GLUT9, OAT1, and OAT3 in the HUA quail kidneys (n = 3).

SLC22A6 Antibody - FIGURE 6 DTX decreased the expression of GLUT9 and increased the expression of OAT1 and OAT3 in rat kidneys (n = 3).

产品:	SLC22A6 抗体
货号:	DF8582
描述:	Rabbit polyclonal antibody to SLC22A6
应用:	WB IHC IF/ICC
文献验证:	WB, IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Horse, Sheep, Rabbit, Dog
分子量:	61 kDa; 62kD(Calculated).
蛋白号:	Q4U2R8
RRID:	AB_2841786

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

联系销售

MSDS

说明书

COA

实验方案

WB Handbook

IHC Protocol

IF/ICC Protocol

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human, Mouse, Rat

预测:

Pig(100%), Horse(%), Sheep(%), Rabbit(%), Dog(%)

克隆:

Polyclonal

特异性:

SLC22A6 Antibody detects endogenous levels of total SLC22A6.

RRID:

AB_2841786
引用格式: Affinity Biosciences Cat# DF8582, RRID:AB_2841786.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

FLJ55736; hOAT1; hPAHT; hROAT1; MGC45260; OAT1; Organic anion transporter 1; OTTHUMP00000236796; OTTHUMP00000236797; OTTHUMP00000236798; OTTHUMP00000236799; PAH transporter; PAHT; Para aminohippurate transporter; Renal organic anion transporter 1; ROAT1; S22A6_HUMAN; SLC22A6; Solute carrier family 22 (organic anion transporter) member 6; Solute carrier family 22 member 6;

抗原和靶标

免疫原:

Uniprot:

Q4U2R8(S22A6_HUMAN) >>Visit HPA database.

基因/基因ID:

SLC22A6(9356)

表达:

Q4U2R8 S22A6_HUMAN:

Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.

序列:

Q4U2R8 S22A6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAFNDLLQQVGGVGRFQQIQVTLVVLPLLLMASHNTLQNFTAAIPTHHCRPPADANLSKNGGLEVWLPRDRQGQPESCLRFTSPQWGLPFLNGTEANGTGATEPCTDGWIYDNSTFPSTIVTEWDLVCSHRALRQLAQSLYMVGVLLGAMVFGYLADRLGRRKVLILNYLQTAVSGTCAAFAPNFPIYCAFRLLSGMALAGISLNCMTLNVEWMPIHTRACVGTLIGYVYSLGQFLLAGVAYAVPHWRHLQLLVSAPFFAFFIYSWFFIESARWHSSSGRLDLTLRALQRVARINGKREEGAKLSMEVLRASLQKELTMGKGQASAMELLRCPTLRHLFLCLSMLWFATSFAYYGLVMDLQGFGVSIYLIQVIFGAVDLPAKLVGFLVINSLGRRPAQMAALLLAGICILLNGVIPQDQSIVRTSLAVLGKGCLAASFNCIFLYTGELYPTMIRQTGMGMGSTMARVGSIVSPLVSMTAELYPSMPLFIYGAVPVAASAVTVLLPETLGQPLPDTVQDLESRWAPTQKEAGIYPRKGKQTRQQQEHQKYMVPLQASAQEKNGL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Rabbit

100

Pig

100

Horse

Sheep

Dog

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

Q4U2R8_S22A6_HUMAN

功能:

Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS) (By similarity). Mediates the sodium-independent uptake of p-aminohippurate (PAH), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP) and edaravone sulfate. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), sulindac, diclofenac, carprofen, glutarate and okadaic acid (By similarity). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, furosemide, indomethacin, bumetamide, losartan, probenecid, phenol red, urate, and alpha-ketoglutarate.

翻译修饰:

Glycosylated. Glycosylation at Asn-113 may occur at a secondary level. Glycosylation is necessary for proper targeting of the transporter to the plasma membrane.

细胞定位:

Cell membrane>Multi-pass membrane protein.

组织特异性:

Strongly expressed in kidney and to a lower extent in liver, skeletal muscle, brain and placenta. Found at the basolateral membrane of the proximal tubule.

蛋白家族:

Multiple cysteine residues are necessary for proper targeting to the plasma membrane.

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

文献引用

1). Ameliorative action of "daitongxiao" against hyperuricemia includes the "uric acid transporter group". Frontiers in pharmacology, 2024 (PubMed: 38983915) [IF=5.6]

Application: WB Species: quail Sample: kidneys

FIGURE 4 DTX treatment decreased the expression of GLUT9, OAT1, and OAT3 in the HUA quail kidneys (n = 3). The melting curves of RT-PCR products of renal (A) GLUT9, (B) OAT3, (C) GAPDH, (D) OAT1, and (E) β-actin in the quail kidneys. The amplification curve of the RT-PCR products of (F) GLUT9, (G) OAT3, (H) GAPDH, (I) OAT1, and (J) β-actin in the quail kidneys. The bar diagram showing the relative expression levels of (K) GLUT9 mRNA, (L) OAT3 mRNA, and (M) OAT1 mRNA. (N) Results of Western blotting showing the relative expression levels of GLUT9, OAT1, OAT3, and GAPDH in the kidneys of control, HUA model, and drug-treated quails. An equal amount (75 µg/lane) of total protein was loaded in each lane of 10% SDS-polyacrylamide gel, fractionated, and then analyzed. The bar diagram shows the relative expression levels of the (O) GLUT9 protein, (P) OAT1 protein, and (Q) OAT3 protein in the quail kidneys. Relative expression was estimated after normalizing with GAPDH/β-actin. #p < 0.05 and ##p < 0.01 compared with the control group. *p < 0.05 and **p < 0.01 compared with the model group; ns: p > 0.05.

Application: IF/ICC Species: Rat Sample: kidneys

FIGURE 6 DTX decreased the expression of GLUT9 and increased the expression of OAT1 and OAT3 in rat kidneys (n = 3). The melting curve of the RT-PCR product of renal (A) GLUT9, (B) OAT1, (C) OAT3, and (D) GAPDH of rats. The amplification curve of the RT-PCR product of renal (E) GLUT9, (F) OAT1, (G) OAT3, and (H) GAPDH of rats. The representative photomicrographs showing the immunodetection of (I) GLUT9, (J) OAT1, and (K) OAT3 protein expression in rat kidney sections. The bar diagrams show the relative expression levels of renal (L) GLUT9 mRNA, (M) OAT1 mRNA, (N) OAT3 mRNA, (O) GLUT9 protein, (P) OAT1 protein, and (Q) OAT3 protein in control, HUA, and DTX-treated HUA rats. The mean fluorescent intensity was measured across the selected region of each subfigure of immunofluorescence. Compared with the control group, #p < 0.05 and ##p < 0.01. Compared with the model group, *p < 0.05 and **p < 0.01.

2). Hypouricemic effect of gallic acid, a bioactive compound from Sonneratia apetala leaves and branches, on hyperuricemic mice. Food & Function, 2022 (PubMed: 36125096) [IF=5.1]

3). Hypouricaemic and nephroprotective effects of Poria cocos in hyperuricemic mice by up-regulating ATP-binding cassette super-family G member 2. PHARMACEUTICAL BIOLOGY, 2021 (PubMed: 33651969) [IF=3.9]

Application: WB Species: Mice Sample: kidney tissues

Figure 4. Effects of PCE and PCW on renal ABCG2, OAT3, OAT1 and OCT2 protein expression detected by Western blot: immunoreactive bands (a) and densitometries (b,c,d and –e, expressed as mean ± SD; n n ¼ 3). p < 0.05, p < 0.01 versus the normal control; #p < 0.05, ##p < 0.01 versus the hyperuricemic control; p < 0.01 versus the allopurinol control.

4). Ethanol extract of Nepeta angustifoia C. Y. Wu ameliorates hyperuricemia in fructose-induced mice. Pakistan journal of pharmaceutical sciences, 2022 (PubMed: 36008897) [IF=0.7]

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SLC22A6 Antibody - #DF8582