Netrin-1 Antibody - #DF8579

Figure 7 A DAPK1 inhibitor prevents the PTBP1 knockdown induced reprogramming. (A-E) Western blot study of NTN1, DAPK1, P-DAPK1(Ser308), P-P53(ser20), and caspase 3(p17) protein in U251 cells infected with sh-Luci and sh-PTBP1 for 3 and 7 days. (n = 3). As an internal reference protein, GAPDH was used. (F) Activity of PP2A during reprogramming. (n = 3). (G-I) TUJ1 and KI67 positive rates of six distinct groups constituted of lentivirus (sh-Luci or sh-PTBP1) and TC-DAPK6 (vehicle, 100 nM, 250 nM, and 500 nM; nine random fields from triplicate samples were recorded for quantification; TUJ1+ (%) = TUJ1+ M-cherry+/M-cherry+; KI67+ (%) = KI67+ M-cherry+/M-cherry+; M-cherry+ cells = 156-553 for each condition). The data are presented as mean ± SD. *P < 0.05, *** P < 0.001 vs. sh-Luci-3d or sh-PTBP1 + vehicle group. Dpi (d): days post infection; ND: not detected; NS: no significance. Scale: 100 µm.

FIGURE 1 KOA synovial fibrosis activates vascular endothelial cells to promote Netrin-1 secretion. (A) Each group was stained with Sirius Red stain and Masson stain for rat synovial tissue, 200× scale bar = 50 μm, showing collagen fibre content. Each group was stained with synovial tissue using silver plated nerve, 200x scale bar = 50 μm, showing neuronal and nerve fibre content. (B) Each group used Netrin-1 + CD31 immunofluorescence staining of synovial tissue, 100× scale bar = 100 μm, to show the expression of the vascular endothelial cell marker CD31 and the expression of Netrin-1 in synovial tissue. (C–E) WB and PCR were performed to detect the protein and gene expression of Netrin-1 and vascular endothelial cell activation markers ICAM-1 and VCAM-1 in the synovial tissues of rats in each group. (*p 

FIGURE 1 KOA synovial fibrosis activates vascular endothelial cells to promote Netrin-1 secretion. (A) Each group was stained with Sirius Red stain and Masson stain for rat synovial tissue, 200× scale bar = 50 μm, showing collagen fibre content. Each group was stained with synovial tissue using silver plated nerve, 200x scale bar = 50 μm, showing neuronal and nerve fibre content. (B) Each group used Netrin-1 + CD31 immunofluorescence staining of synovial tissue, 100× scale bar = 100 μm, to show the expression of the vascular endothelial cell marker CD31 and the expression of Netrin-1 in synovial tissue. (C–E) WB and PCR were performed to detect the protein and gene expression of Netrin-1 and vascular endothelial cell activation markers ICAM-1 and VCAM-1 in the synovial tissues of rats in each group. (*p 

Fig. 1(A) Western blotting analysis of Netrin-1 and GAPDH and relative changes in Netrin-1 expression with respect to GAPDH expression, reported as the Netrin-1/GAPDH ratio, in the cerebral cortexes of rats injected with ferric–tannic nanoparticles (FTs) at different times (n = 2). P = 0.0667, by one-way analysis of variance (ANOVA) with Kruskal–Wallis test. (B) Netrin-1 immunofluorescence staining of the cerebral cortex of control rats and rats injected with FTs at different times. (C) Individual quantitative analysis of Netrin-1 staining of four different areas of the cerebral cortex of control rats (n = 2) and five different areas of the cerebral cortex of rats injected with FTs for 48 hours (n = 2). (D) Semiquantitative analysis of Netrin-1 staining of the cerebral cortex of control rats (n = 2, total number of measured cells was 378), and rats injected with FTs for 48 hours (n = 2, total number of measured cells was 512). ****P < 0.0001 by unpaired t-test. (E) Western blotting analysis of GABRA4 and GAPDH and relative changes in Netrin-1 expression with respect to GAPDH expression, reported as the GABRA4/GAPDH ratio, in the cerebral cortex of rats injected with FTs at different times (n = 2). P = 0.933, by one-way ANOVA with Kruskal–Wallis test. (F) GABRA4 immunofluorescence staining of the cerebral cortex of control rats and rats injected with FTs at different times. (G) Individual quantitative analysis of GABRA4 staining of seven different areas of the cerebral cortex of control rats (n = 2) and five different areas of the cerebral cortex of rats injected with FTs for 48 hours (n = 2). (H) Semiquantitative analysis of Netrin-1 staining of the cerebral cortex of control rats (n = 2, total number of measured cells was 636), and rats injected with FTs for 48 hours (n = 2, total number of measured cells was 502). ****P < 0.0001 by unpaired t-test.