Nav1.6/SCN8A Antibody - #DF8577

Figure 7. FGF13 participates in BCP by regulating sodium channels. (A) qPCR for monitoring FGF13-siRNA (siRNAFgf13) in RT4-D6P2T cells to validate transfection efficiency. Bars represent the means ± SEM of independent biological replicates (n = 3). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (B) Western blots for monitoring transfection efficiency of siRNAFgf13 in BCP rat SDH were validated. Bars represent the means ± SEM of independent biological replicates (n = 3). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (***P < 0.001). (C) Reducing Fgf13 expression attenuates mechanical nociceptive sensitization in BCP rats. Bars represent the means ± SEM of independent biological replicates (n = 8). P-values were calculated using the two-way repeated measures analysis (###P < 0.001). PWT: Paw withdrawal threshold. (D) Statistical plots of the number of buried strains in Sham, BCP+siRNANC and BCP+ siRNAFgf13 groups. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (E-G) Representative CatWalk gait, including footprint area and mean intensity (E), in Sham, BCP+ siRNANC and BCP+ siRNAFgf13 groups. Quantifications of mean intensity (LH/RH) (F) and print area (LH/RH) (G) in both groups of rats. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the Student's t test (**P < 0.01; ***P < 0.001). LH, left hind paw; RH, right hind paw. (H-J) Representative traces of locomotor activity in the light-dark transition (LDT) (H) in Sham group, BCP+siRNANC group and BCP+siRNAFgf13 group rats. Quantifications of time spent (I) and distance (J) in the light chamber. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (K-L) Representative traces of Elevated plus- maze (EPM) (K) in Sham group, BCP+siRNANC group and BCP+siRNAFgf13 group rats. Quantifications of time spent (L) in the open arms. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (***P < 0.001). (M-P) Western blots for monitoring the effect of siRNAFgf13 on the expression of Nav1.1, Nav1.6, Nav1.7, Nav1.8 in the SDH of BCP rats. ß-Actin served as loading control. Bars represent the means ± SEM of independent biological replicates (n = 4). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (*P < 0.05; **P < 0.01).