Phospho-Dab1 (Tyr232) Antibody - #AF3029

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产品: 磷酸化 Dab1 (Tyr232) 抗体
货号: AF3029
描述: Rabbit polyclonal antibody to Phospho-Dab1 (Tyr232)
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 60kDa; 64kD(Calculated).
蛋白号: O75553
RRID: AB_2834459

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
Phospho-Dab1 (Tyr232) Antibody detects endogenous levels of Dab1 only when phosphorylated at Tyrosine 232.
RRID:
AB_2834459
引用格式: Affinity Biosciences Cat# AF3029, RRID:AB_2834459.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Dab 1; Dab reelin signal transducer 1; Dab, reelin signal transducer, homolog 1 (Drosophila); Dab1; DAB1_HUMAN; Disabled homolog 1; Disabled homolog 1 Drosophila; Scm; Scr; Scrambler; Yot; Yotari;

抗原和靶标

免疫原:

A synthesized peptide derived from human Dab1 around the phosphorylation site of Tyr232.

Uniprot:

O75553(DAB1_HUMAN) >>Visit HPA database.

基因/基因ID:
DAB1(1600)
表达:
O75553 DAB1_HUMAN:

Mainly expressed in brain.

描述:
DAB1 an adaptor molecule functioning in neural development. The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer.
序列:
MSTETELQVAVKTSAKKDSRKKGQDRSEATLIKRFKGEGVRYKAKLIGIDEVSAARGDKLCQDSMMKLKGVVAGARSKGEHKQKIFLTISFGGIKIFDEKTGALQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVAIKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPIRDPETEENIYQVPTSQKKEGVYDVPKSQPVSNGYSFEDFEERFAAATPNRNLPTDFDEIFEATKAVTQLELFGDMSTPPDITSPPTPATPGDAFIPSSSQTLPASADVFSSVPFGTAAVPSGYVAMGAVLPSFWGQQPLVQQQMVMGAQPPVAQVMPGAQPIAWGQPGLFPATQQPWPTVAGQFPPAAFMPTQTVMPLPAAMFQGPLTPLATVPGTSDSTRSSPQTDKPRQKMGKETFKDFQMAQPPPVPSRKPDQPSLTCTSEAFSSYFNKVGVAQDTDDCDDFDISQLNLTPVTSTTPSTNSPPTPAPRQSSPSKSSASHASDPTTDDIFEEGFESPSKSEEQEAPDGSQASSNSDPFGEPSGEPSGDNISPQAGS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Adapter molecule functioning in neural development. May regulate SIAH1 activity.

翻译修饰:

Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling.

组织特异性:

Mainly expressed in brain.

蛋白家族:

The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.

文献引用

1). Ephrin‑b3 modulates hippocampal neurogenesis and the reelin signaling pathway in a pilocarpine‑induced model of epilepsy. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2018 (PubMed: 29512697) [IF=5.7]

Application: WB    Species: rat    Sample: hippocampus

Figure 3. |Reelin pathway expression in the hippocampus of rats during the development of SE.(E) Western blot analysis for p‑Dab1. β‑actin was used as an endogenous control. (F) Quantification of western blot results of p‑Dab1, presented as the p‑Dab1/Dab1 ratio. Data are presented as the mean ± standard deviation. ***P<0.0001, **P<0.001 by two‑way analysis of variance with Tukey's post hoc test (n=3 rats/per group). Ep, epilepsy; Con, control; Dab1, disabled 1; p‑Dab, phosphorylated Dab1.

Application: IHC    Species: rat    Sample: hippocampus

Figure 5. |Effect of EphB3‑Fc on reelin pathway expression in the hippocampus post‑SE.(C) Representative immunohistochemical staining of p‑Dab1 following EphB3‑Fc/Fc‑control treatment. Left: representative immunohistochemical staining of p‑Dab1 (scale bar=50 µm); right: high‑magnification bright‑field images and the arrows indicate the immunopositive cells (scale bar=10 µm).

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