IDH2 Antibody - #DF8561

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产品: IDH2 抗体
货号: DF8561
描述: Rabbit polyclonal antibody to IDH2
应用: WB IF/ICC IHC-P
反应: Human, Mouse, Rat, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 50 kDa; 51kD(Calculated).
蛋白号: P48735
RRID: AB_2841765

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat,Monkey
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%)
克隆:
Polyclonal
特异性:
IDH2 Antibody detects endogenous levels of total IDH2.
RRID:
AB_2841765
引用格式: Affinity Biosciences Cat# DF8561, RRID:AB_2841765.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

D2HGA2; ICD-M; IDH; IDH2; IDHM; IDHP_HUMAN; IDP; IDPM; Isocitrate dehydrogenase [NADP], mitochondrial; Isocitrate dehydrogenase 2 (NADP+), mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase;

抗原和靶标

免疫原:
Uniprot:

P48735(IDHP_HUMAN) >>Visit HPA database.

基因/基因ID:
IDH2(3418)
序列:
MAGYLRVVRSLCRASGSRPAWAPAALTAPTSQEQPRRHYADKRIKVAKPVVEMDGDEMTRIIWQFIKEKLILPHVDIQLKYFDLGLPNRDQTDDQVTIDSALATQKYSVAVKCATITPDEARVEEFKLKKMWKSPNGTIRNILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVAQVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTRHYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAMTKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Xenopus
71
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P48735 作为底物

Site PTM Type Enzyme
K48 Acetylation
K67 Acetylation
K69 Acetylation
K69 Ubiquitination
K80 Acetylation
K80 Ubiquitination
T97 Phosphorylation
S100 Phosphorylation
K106 Acetylation
K106 Ubiquitination
Y107 Phosphorylation
S108 Phosphorylation
K112 Ubiquitination
T115 Phosphorylation
T117 Phosphorylation
K127 Acetylation
K127 Ubiquitination
K133 Acetylation
S134 Phosphorylation
T146 Phosphorylation
K155 Acetylation
K155 Ubiquitination
K166 Acetylation
K166 Ubiquitination
Y179 Phosphorylation
K180 Acetylation
K180 Ubiquitination
T182 Phosphorylation
R188 Methylation
T191 Phosphorylation
K193 Ubiquitination
T197 Phosphorylation
K199 Ubiquitination
K242 Acetylation
Y247 Phosphorylation
K256 Acetylation
K256 Ubiquitination
K263 Acetylation
K263 Ubiquitination
K272 Acetylation
K272 Ubiquitination
K275 Acetylation
K275 Ubiquitination
K280 Acetylation
K282 Acetylation
K282 Ubiquitination
S301 Phosphorylation
C308 S-Nitrosylation
T350 Phosphorylation
K384 Acetylation
K384 Ubiquitination
K400 Ubiquitination
K413 Acetylation
K413 Ubiquitination
S423 Phosphorylation
K426 Ubiquitination
K442 Acetylation
K442 Ubiquitination

研究背景

功能:

Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex.

翻译修饰:

Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3.

细胞定位:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer.

蛋白家族:

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

文献引用

1). Molecular mechanism of albumin in suppressing invasion and metastasis of hepatocellular carcinoma. LIVER INTERNATIONAL (PubMed: 34854209) [IF=6.7]

Application: WB    Species: Human    Sample: HepG2 cells

FIGURE 5 Verification of the differentially expressed proteins. A, the RT‐PCR detected the relative mRNA expression levels of AZGP1, GPC3, CTSF, NOTCH1, CTSD, uPAR, BMP2, LCN2, IDH2 and RPS3A (*P < .05). B, A representative western blot analysis for AZGP1, GPC3, NOTCH1, CTSD, uPAR, BMP2, LCN2, IDH2 and RPS3A expression in intracellular and extracellular samples from HepG2 cells. (Bars indicate SD, *P < .05)

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