(2)
(7)
产品描述
来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse
克隆:
Polyclonal
特异性:
Involucrin Antibody detects endogenous levels of total Involucrin.
RRID:
AB_2833379
引用格式: Affinity Biosciences Cat# AF0186, RRID:AB_2833379.
引用格式: Affinity Biosciences Cat# AF0186, RRID:AB_2833379.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
INVO_HUMAN; Involucrin; IVL;
抗原和靶标
免疫原:
A synthesized peptide derived from human Involucrin
基因/基因ID:
描述:
IVL Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family. Directly or indirectly cross-linked to cornifelin (CNFN).
文献引用
1). Use of mouse primary epidermal organoids for USA300 infection modeling and drug screening. Research Square, 2022
(PubMed: 36631452)
[IF=8.1]
Application: IHC Species: Mouse Sample: mPEOs
Fig. 1: Characterization of mouse primary epidermal organoids (mPEOs).A Overview of mPEOs formed from single cells within 7 d (scale bars, 100 μm). B Increased number of mPEOs observed within 7 d in bright field (scale bars, 100 μm). C H&E staining of mPEOs cultured for 7 d (scale bars, 50 μm). D Ultrathin sections in TEM images of mPEOs (scale bars, 20 μm), SB stratum basale, SP stratum spinosum, GR stratum granulosum, KG keratohyalin granules, SC stratum corneum. E Immunofluorescence staining analysis of various skin-associated specific biomarkers in mPEOs (scale bars, 200 μm). F Immunohistochemical staining of paraffin-embedded mPEOs. Representative images of Loricrin, KRT10, Involucrin, KRT14 and Ki67 immunoreactivity in organoids (scale bars, 50 μm).
2). GDU-952, a novel AhR agonist ameliorates skin barrier abnormalities and immune dysfunction in DNFB-induced atopic dermatitis in mice. Biochemical pharmacology, 2023
(PubMed: 37778446)
[IF=5.3]
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