产品: Involucrin 抗体
货号: AF0186
描述: Rabbit polyclonal antibody to Involucrin
应用: WB IHC IF/ICC
反应: Human, Mouse
分子量: 68~120kDa; 68kD(Calculated).
蛋白号: P07476
RRID: AB_2833379

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
Involucrin Antibody detects endogenous levels of total Involucrin.
RRID:
AB_2833379
引用格式: Affinity Biosciences Cat# AF0186, RRID:AB_2833379.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

INVO_HUMAN; Involucrin; IVL;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P07476 INVO_HUMAN:

Keratinocytes of epidermis and other stratified squamous epithelia.

描述:
IVL Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family. Directly or indirectly cross-linked to cornifelin (CNFN).
序列:
MSQQHTLPVTLSPALSQELLKTVPPPVNTHQEQMKQPTPLPPPCQKVPVELPVEVPSKQEEKHMTAVKGLPEQECEQQQKEPQEQELQQQHWEQHEEYQKAENPEQQLKQEKTQRDQQLNKQLEEEKKLLDQQLDQELVKRDEQLGMKKEQLLELPEQQEGHLKHLEQQEGQLKHPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPQQQEGQLELSEQQEGQLELSEQQEGQLKHLEHQEGQLEVPEEQMGQLKYLEQQEGQLKHLDQQEKQPELPEQQMGQLKHLEQQEGQPKHLEQQEGQLEQLEEQEGQLKHLEQQEGQLEHLEHQEGQLGLPEQQVLQLKQLEKQQGQPKHLEEEEGQLKHLVQQEGQLKHLVQQEGQLEQQERQVEHLEQQVGQLKHLEEQEGQLKHLEQQQGQLEVPEQQVGQPKNLEQEEKQLELPEQQEGQVKHLEKQEAQLELPEQQVGQPKHLEQQEKHLEHPEQQDGQLKHLEQQEGQLKDLEQQKGQLEQPVFAPAPGQVQDIQPALPTKGEVLLPVEHQQQKQEVQWPPKHK

翻译修饰 - P07476 作为底物

Site PTM Type Enzyme
S12 Phosphorylation
S16 Phosphorylation
T65 Phosphorylation
K314 Acetylation

研究背景

功能:

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

翻译修饰:

Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

细胞定位:

Cytoplasm.
Note: Constituent of the scaffolding of the cornified envelope.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Keratinocytes of epidermis and other stratified squamous epithelia.

亚基结构:

Directly or indirectly cross-linked to cornifelin (CNFN).

蛋白家族:

Belongs to the involucrin family.

文献引用

1). Xie et al. Use of mouse primary epidermal organoids for USA300 infection modeling and drug screening. Research Square 2023 Jan 11;14(1):15. (PubMed: 36631452) [IF=9.0]

Application: IHC    Species: Mouse    Sample: mPEOs

Fig. 1: Characterization of mouse primary epidermal organoids (mPEOs).A Overview of mPEOs formed from single cells within 7 d (scale bars, 100 μm). B Increased number of mPEOs observed within 7 d in bright field (scale bars, 100 μm). C H&E staining of mPEOs cultured for 7 d (scale bars, 50 μm). D Ultrathin sections in TEM images of mPEOs (scale bars, 20 μm), SB stratum basale, SP stratum spinosum, GR stratum granulosum, KG keratohyalin granules, SC stratum corneum. E Immunofluorescence staining analysis of various skin-associated specific biomarkers in mPEOs (scale bars, 200 μm). F Immunohistochemical staining of paraffin-embedded mPEOs. Representative images of Loricrin, KRT10, Involucrin, KRT14 and Ki67 immunoreactivity in organoids (scale bars, 50 μm).

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