| 产品: | Brm 抗体 |
| 货号: | DF8509 |
| 描述: | Rabbit polyclonal antibody to Brm |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 181 kDa; 181kD(Calculated). |
| 蛋白号: | P51531 |
| RRID: | AB_2841715 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF8509, RRID:AB_2841715.
展开/折叠
ATP dependent helicase SMARCA2; ATP-dependent helicase SMARCA2; BAF190; BAF190B; BRG1-associated factor 190B; BRM; FLJ36757; Global transcription activator homologous sequence; hBRM; hSNF2a; MGC74511; Possible global transcription activator SNF2L2; Probable global transcription activator SNF2L2; Protein brahma homolog; SMARCA2; SMCA2_HUMAN; SNF2 alpha; SNF2 like 2; SNF2-alpha; SNF2/SWI2 like protein 2; SNF2L2; SNF2LA; Sth1p; Sucrose nonfermenting 2 like protein 2; SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 2; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2;
抗原和靶标
- P51531 SMCA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTPTDPGAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVSHPMPTMGSTDFPQEGMHQMHKPIDGIHDKGIVEDIHCGSMKGTGMRPPHPGMGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPMSGGGPTPPQMPPSQPGALIPGDPQAMSQPNRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKRTLPGLQQQQQQQQQQQQQQQQQQQQQQQPQQQPPQPQTQQQQQPALVNYNRPSGPGPELSGPSTPQKLPVPAPGGRPSPAPPAAAQPPAAAVPGPSVPQPAPGQPSPVLQLQQKQSRISPIQKPQGLDPVEILQEREYRLQARIAHRIQELENLPGSLPPDLRTKATVELKALRLLNFQRQLRQEVVACMRRDTTLETALNSKAYKRSKRQTLREARMTEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSVAGKIQKLSKAVATWHANTEREQKKETERIEKERMRRLMAEDEEGYRKLIDQKKDRRLAYLLQQTDEYVANLTNLVWEHKQAQAAKEKKKRRRRKKKAEENAEGGESALGPDGEPIDESSQMSDLPVKVTHTETGKVLFGPEAPKASQLDAWLEMNPGYEVAPRSDSEESDSDYEEEDEEEESSRQETEEKILLDPNSEEVSEKDAKQIIETAKQDVDDEYSMQYSARGSQSYYTVAHAISERVEKQSALLINGTLKHYQLQGLEWMVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRLNGPYLIIVPLSTLSNWTYEFDKWAPSVVKISYKGTPAMRRSLVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRILLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGERVDLNEEETILIIRRLHKVLRPFLLRRLKKEVESQLPEKVEYVIKCDMSALQKILYRHMQAKGILLTDGSEKDKKGKGGAKTLMNTIMQLRKICNHPYMFQHIEESFAEHLGYSNGVINGAELYRASGKFELLDRILPKLRATNHRVLLFCQMTSLMTIMEDYFAFRNFLYLRLDGTTKSEDRAALLKKFNEPGSQYFIFLLSTRAGGLGLNLQAADTVVIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEENEEEDEVPDDETLNQMIARREEEFDLFMRMDMDRRREDARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEEEKIFGRGSRQRRDVDYSDALTEKQWLRAIEDGNLEEMEEEVRLKKRKRRRNVDKDPAKEDVEKAKKRRGRPPAEKLSPNPPKLTKQMNAIIDTVINYKDRCNVEKVPSNSQLEIEGNSSGRQLSEVFIQLPSRKELPEYYELIRKPVDFKKIKERIRNHKYRSLGDLEKDVMLLCHNAQTFNLEGSQIYEDSIVLQSVFKSARQKIAKEEESEDESNEEEEEEDEEESESEAKSVKVKIKLNKKDDKGRDKGKGKKRPNRGKAKPVVSDFDSDEEQDEREQSEGSGTDDE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P51531 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S93 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S175 | Phosphorylation | Uniprot | |
| K190 | Acetylation | Uniprot | |
| R194 | Methylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| T408 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| Y415 | Phosphorylation | Uniprot | |
| K437 | Acetylation | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| R505 | Methylation | Uniprot | |
| Y514 | Phosphorylation | Uniprot | |
| T541 | Phosphorylation | Uniprot | |
| S587 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| S591 | Phosphorylation | Uniprot | |
| K604 | Acetylation | Uniprot | |
| K604 | Ubiquitination | Uniprot | |
| Y642 | Phosphorylation | Uniprot | |
| S651 | Phosphorylation | Uniprot | |
| S666 | Phosphorylation | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| Y689 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| K842 | Ubiquitination | Uniprot | |
| S956 | Phosphorylation | Uniprot | |
| K961 | Ubiquitination | Uniprot | |
| K967 | Ubiquitination | Uniprot | |
| S971 | Phosphorylation | Uniprot | |
| K996 | Acetylation | Uniprot | |
| K997 | Acetylation | Uniprot | |
| K999 | Acetylation | Uniprot | |
| K1003 | Acetylation | Uniprot | |
| T1004 | Phosphorylation | Uniprot | |
| T1008 | Phosphorylation | Uniprot | |
| Y1020 | Phosphorylation | Uniprot | |
| Y1035 | Phosphorylation | Uniprot | |
| Y1046 | Phosphorylation | Uniprot | |
| K1051 | Ubiquitination | Uniprot | |
| R1057 | Methylation | Uniprot | |
| K1183 | Ubiquitination | Uniprot | |
| K1189 | Acetylation | Uniprot | |
| K1189 | Ubiquitination | Uniprot | |
| Y1190 | Phosphorylation | Uniprot | |
| K1191 | Ubiquitination | Uniprot | |
| K1197 | Sumoylation | Uniprot | |
| K1197 | Ubiquitination | Uniprot | |
| K1207 | Ubiquitination | Uniprot | |
| K1271 | Acetylation | Uniprot | |
| S1282 | Phosphorylation | Uniprot | |
| K1286 | Sumoylation | Uniprot | |
| K1286 | Ubiquitination | Uniprot | |
| T1295 | Phosphorylation | Uniprot | |
| K1302 | Ubiquitination | Uniprot | |
| S1308 | Phosphorylation | Uniprot | |
| Y1316 | Phosphorylation | Uniprot | |
| K1323 | Ubiquitination | Uniprot | |
| S1377 | Phosphorylation | Uniprot | |
| S1408 | Phosphorylation | Uniprot | |
| S1410 | Phosphorylation | Uniprot | |
| S1418 | Phosphorylation | Uniprot | |
| S1419 | Phosphorylation | Uniprot | |
| K1434 | Ubiquitination | Uniprot | |
| Y1439 | Phosphorylation | Uniprot | |
| S1512 | Phosphorylation | Uniprot | |
| S1516 | Phosphorylation | Uniprot | |
| S1528 | Phosphorylation | Uniprot | |
| K1547 | Acetylation | Uniprot | |
| K1551 | Acetylation | Uniprot | |
| K1553 | Acetylation | Uniprot | |
| K1555 | Acetylation | Uniprot | |
| K1556 | Acetylation | Uniprot | |
| S1568 | Phosphorylation | Uniprot | |
| S1572 | Phosphorylation | Uniprot | |
| S1582 | Phosphorylation | Uniprot | |
| S1585 | Phosphorylation | Uniprot | |
| T1587 | Phosphorylation | Uniprot |
研究背景
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
Nucleus.
Component of the multiprotein chromatin-remodeling complexes SWI/SNF: SWI/SNF-A (BAF), SWI/SNF-B (PBAF) and related complexes. The canonical complex contains a catalytic subunit (either SMARCA4/BRG1/BAF190A or SMARCA2/BRM/BAF190B) and at least SMARCE1, ACTL6A/BAF53, SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1/SNF5/BAF47. Other subunits specific to each of the complexes may also be present permitting several possible combinations developmentally and tissue specific (Probable). Component of the BAF complex, which includes at least actin (ACTB), ARID1A/BAF250A, ARID1B/BAF250B, SMARCA2/BRM, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Component of neuron-specific chromatin remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B, DPF3/BAF45C, ACTL6B/BAF53B and actin. Interacts with PHF10/BAF45A (By similarity). Interacts with CEBPB (when not methylated). Interacts with TOPBP1. Interacts with CEBPA (when phosphorylated). Interacts with DPF2. Interacts with ERCC6.
Belongs to the SNF2/RAD54 helicase family.
研究领域
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
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