Phospho-Aurora A (Thr288) Antibody - #AF3011

Figure 5 Aurora A-mediated primary cilia disassembly inhibits human decidualization. (A) Relative mRNA level of AURKA during stromal cells decidualization for 2, 4, and 6 days. (B,C) Western blot and quantification of p-Aurora A (T288) during stromal cells decidualization for 2, 4, and 6 days. (D) ARL13B immunofluorescence in AMG-900-treated stromal cells for 48 h. Scale bars, 5 μm. (E,F) Ciliated cells and cilia length were measured in AMG-900-treated stromal cells for 48 h. (G,H) Relative mRNA levels of IGFBP1 and PRL in AMG-900-treated stromal cells for 48 h. (I) ARL13B immunofluorescence in TC-S 7010-treated stromal cells for 48 h. Scale bars, 5 μm. (J,K) Ciliated cells and cilia length were measured in TC-S 7010-treated stromal cells for 48 h. (L,M) Relative mRNA levels of IGFBP1 and PRL in TC-S 7010-treated stromal cells for 48 h. In (B,D,I), the representative images of three biologically independent experiments are shown. In (F,K), these results are pooled from three independent experiments and presented as violin plots. The thick bar indicates the median and the dotted lines the first and third quartiles. In (A,C,E–H,J–M), data are presented as means ± SD from three independent experiments. ns, (p > 0.05), * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 by two-tailed Student’s t test for comparing two groups or one-way ANOVA test for comparing more than two groups.