产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3011, RRID:AB_2834450.
展开/折叠
AIK; ARK-1; ARK1; AURA; Aurka; Aurora 2; Aurora A; Aurora kinase A; Aurora-related kinase 1; Aurora/IPL1 like kinase; Aurora/IPL1-related kinase 1; AURORA2; Breast tumor-amplified kinase; BTAK; hARK1; IAK; IPL1 related kinase; MGC34538; OTTHUMP00000031340; OTTHUMP00000031341; OTTHUMP00000031342; OTTHUMP00000031343; OTTHUMP00000031344; OTTHUMP00000031345; OTTHUMP00000166071; OTTHUMP00000166072; PPP1R47; Protein phosphatase 1, regulatory subunit 47; Serine/threonine kinase 15; Serine/threonine kinase 6; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; STK15; STK6; STK6_HUMAN; STK7;
抗原和靶标
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
- O14965 AURKA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDRSKENCISGPVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSNSSQRVPLQAQKLVSSHKPVQNQKQKQLQATSVPHPVSRPLNNTQKSKQPLPSAPENNPEEELASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVYLAREKQSKFILALKVLFKAQLEKAGVEHQLRREVEIQSHLRHPNILRLYGYFHDATRVYLILEYAPLGTVYRELQKLSKFDEQRTATYITELANALSYCHSKRVIHRDIKPENLLLGSAGELKIADFGWSVHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLCYEFLVGKPPFEANTYQETYKRISRVEFTFPDFVTEGARDLISRLLKHNPSQRPMLREVLEHPWITANSSKPSNCQNKESASKQS
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14965 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S4 | Phosphorylation | Uniprot | |
S10 | Phosphorylation | O14965 (AURKA) | Uniprot |
T16 | Phosphorylation | O14965 (AURKA) | Uniprot |
K23 | Ubiquitination | Uniprot | |
S41 | Phosphorylation | O14965 (AURKA) | Uniprot |
S51 | Phosphorylation | Uniprot | |
S53 | Phosphorylation | Uniprot | |
S54 | Phosphorylation | Uniprot | |
K63 | Ubiquitination | Uniprot | |
S66 | Phosphorylation | Uniprot | |
S67 | Phosphorylation | O14965 (AURKA) | Uniprot |
K69 | Ubiquitination | Uniprot | |
S83 | Phosphorylation | O14965 (AURKA) | Uniprot |
S89 | Phosphorylation | Uniprot | |
S98 | Phosphorylation | O14965 (AURKA) | Uniprot |
K99 | Ubiquitination | Uniprot | |
S104 | Phosphorylation | O14965 (AURKA) | Uniprot |
S116 | Phosphorylation | Uniprot | |
K117 | Ubiquitination | Uniprot | |
K141 | Ubiquitination | Uniprot | |
K143 | Ubiquitination | Uniprot | |
Y148 | Phosphorylation | P12931 (SRC) , O14965 (AURKA) | Uniprot |
S226 | Phosphorylation | O14965 (AURKA) | Uniprot |
K227 | Ubiquitination | Uniprot | |
K250 | Ubiquitination | Uniprot | |
K258 | Sumoylation | Uniprot | |
K258 | Ubiquitination | Uniprot | |
S266 | Phosphorylation | O14965 (AURKA) | Uniprot |
S278 | Phosphorylation | O14965 (AURKA) | Uniprot |
S283 | Phosphorylation | Uniprot | |
S284 | Phosphorylation | Uniprot | |
T287 | Phosphorylation | Q05513 (PRKCZ) , O14965 (AURKA) | Uniprot |
T288 | Phosphorylation | Q13153 (PAK1) , O14965 (AURKA) , P17612 (PRKACA) | Uniprot |
T292 | Phosphorylation | Uniprot | |
Y295 | Phosphorylation | Uniprot | |
Y334 | Phosphorylation | Uniprot | |
S342 | Phosphorylation | O14965 (AURKA) , Q13153 (PAK1) | Uniprot |
S369 | Phosphorylation | Uniprot | |
S391 | Phosphorylation | O14965 (AURKA) | Uniprot |
翻译修饰 - O14965 作为激酶
Substrate | Site | Source |
---|---|---|
O14777 (NDC80) | S55 | Uniprot |
O14965 (AURKA) | S10 | Uniprot |
O14965 (AURKA) | T16 | Uniprot |
O14965 (AURKA) | S41 | Uniprot |
O14965 (AURKA) | S67 | Uniprot |
O14965 (AURKA) | S83 | Uniprot |
O14965 (AURKA) | S98 | Uniprot |
O14965 (AURKA) | S104 | Uniprot |
O14965 (AURKA) | Y148 | Uniprot |
O14965 (AURKA) | S226 | Uniprot |
O14965 (AURKA) | S266 | Uniprot |
O14965 (AURKA) | S278 | Uniprot |
O14965 (AURKA) | T287 | Uniprot |
O14965 (AURKA) | T288 | Uniprot |
O14965 (AURKA) | S342 | Uniprot |
O14965 (AURKA) | S391 | Uniprot |
O15143 (ARPC1B) | T21 | Uniprot |
O15350 (TP73) | S235 | Uniprot |
O43521 (BCL2L11) | S93 | Uniprot |
O43521 (BCL2L11) | S94 | Uniprot |
O43521 (BCL2L11) | S98 | Uniprot |
O75496 (GMNN) | T25 | Uniprot |
O95983 (MBD3) | S24 | Uniprot |
O95983 (MBD3) | S85 | Uniprot |
P03372 (ESR1) | S167 | Uniprot |
P03372 (ESR1) | S305 | Uniprot |
P04637 (TP53) | S106 | Uniprot |
P04637 (TP53) | S215 | Uniprot |
P04637 (TP53) | S315 | Uniprot |
P06748 (NPM1) | S125 | Uniprot |
P10275 (AR) | S215 | Uniprot |
P10275 (AR) | T282 | Uniprot |
P10275 (AR) | S293 | Uniprot |
P10275 (AR) | S792 | Uniprot |
P11233 (RALA) | S194 | Uniprot |
P12755 (SKI) | S326 | Uniprot |
P12755 (SKI) | S383 | Uniprot |
P25963 (NFKBIA) | S32 | Uniprot |
P25963 (NFKBIA) | S36 | Uniprot |
P30305-3 (CDC25B) | S312 | Uniprot |
P30305-2 (CDC25B) | S339 | Uniprot |
P30305 (CDC25B) | S353 | Uniprot |
P38398-1 (BRCA1) | S308 | Uniprot |
P40337 (VHL) | S72 | Uniprot |
P41208 (CETN2) | S122 | Uniprot |
P41208 (CETN2) | S170 | Uniprot |
P49450 (CENPA) | S7 | Uniprot |
P49736 (MCM2) | S220 | Uniprot |
P49841 (GSK3B) | S9 | Uniprot |
P53350 (PLK1) | T210 | Uniprot |
P53667 (LIMK1) | S307 | Uniprot |
P53667 (LIMK1) | T508 | Uniprot |
P53671 (LIMK2) | S283 | Uniprot |
P53671 (LIMK2) | T494 | Uniprot |
P53671 (LIMK2) | T505 | Uniprot |
P61978 (HNRNPK) | S379 | Uniprot |
P68431 (HIST1H3J) | S11 | Uniprot |
Q02224 (CENPE) | T422 | Uniprot |
Q13158 (FADD) | S203 | Uniprot |
Q13526 (PIN1) | S16 | Uniprot |
Q13563 (PKD2) | S829 | Uniprot |
Q14194 (CRMP1) | T101 | Uniprot |
Q14194 (CRMP1) | T102 | Uniprot |
Q14203 (DCTN1) | S19 | Uniprot |
Q14511 (NEDD9) | S296 | Uniprot |
Q14980 (NUMA1) | T1804 | Uniprot |
Q14980 (NUMA1) | T1811 | Uniprot |
Q14980 (NUMA1) | T1812 | Uniprot |
Q14980 (NUMA1) | S1887 | Uniprot |
Q14980 (NUMA1) | S1969 | Uniprot |
Q14980 (NUMA1) | S1991 | Uniprot |
Q14980 (NUMA1) | S2047 | Uniprot |
Q14980 (NUMA1) | T2084 | Uniprot |
Q14980 (NUMA1) | S2087 | Uniprot |
Q15398 (DLGAP5) | S627 | Uniprot |
Q15398 (DLGAP5) | S725 | Uniprot |
Q15398 (DLGAP5) | S757 | Uniprot |
Q15398 (DLGAP5) | S830 | Uniprot |
Q49MG5 (MAP9) | S305 | Uniprot |
Q49MG5 (MAP9) | S625 | Uniprot |
Q8IX03 (WWC1) | S539 | Uniprot |
Q8NHV4 (NEDD1) | S405 | Uniprot |
Q8TEW0 (PARD3) | S962 | Uniprot |
Q8WV24 (PHLDA1) | S95 | Uniprot |
Q92974 (ARHGEF2) | S886 | Uniprot |
Q96GD4 (AURKB) | T232 | Uniprot |
Q9BQG0 (MYBBP1A) | S1303 | Uniprot |
Q9BXS6 (NUSAP1) | S240 | Uniprot |
Q9GZM8 (NDEL1) | S251 | Uniprot |
Q9HC77 (CENPJ) | S467 | Uniprot |
Q9NRM7 (LATS2) | S83 | Uniprot |
Q9NRM7 (LATS2) | S380 | Uniprot |
Q9NS23-2 (RASSF1) | T202 | Uniprot |
Q9NS23-2 (RASSF1) | S203 | Uniprot |
Q9NS23 (RASSF1) | T206 | Uniprot |
Q9NS23 (RASSF1) | S207 | Uniprot |
Q9NWT8 (AURKAIP1) | S70 | Uniprot |
Q9ULW0 (TPX2) | S121 | Uniprot |
Q9ULW0 (TPX2) | S125 | Uniprot |
Q9UNN5 (FAF1) | S289 | Uniprot |
Q9UNN5 (FAF1) | S291 | Uniprot |
Q9UPY8 (MAPRE3) | S176 | Uniprot |
Q9Y6A5 (TACC3) | S558 | Uniprot |
研究背景
Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis. Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1.
Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Thr-288 can be phosphorylated by several kinases, including PAK and PKA. Protein phosphatase type 1 (PP1) binds AURKA and inhibits its activity by dephosphorylating Thr-288 during mitosis. Phosphorylation at Ser-342 decreases the kinase activity. PPP2CA controls degradation by dephosphorylating Ser-51 at the end of mitosis.
Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome (By similarity). Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. Ubiquitinated by the CUL3-KLHL18 ligase leading to its activation at the centrosome which is required for initiating mitotic entry. Ubiquitination mediated by CUL3-KLHL18 ligase does not lead to its degradation by the proteasome.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Cell projection>Neuron projection.
Note: Detected at the neurite hillock in developing neurons (By similarity). Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase (PubMed:9606188, PubMed:17229885, PubMed:21225229). Colocalized with SIRT2 at centrosome (PubMed:22014574). Moves to the midbody during both telophase and cytokinesis (PubMed:17726514). Associates with both the pericentriolar material (PCM) and centrioles (PubMed:22014574). The localization to the spindle poles is regulated by AAAS (PubMed:26246606).
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Interacts with FBXL7 (By similarity). Interacts with CPEB1, JTB, TACC1, TPX2, PPP2CA, as well as with the protein phosphatase type 1 (PP1) isoforms PPP1CA, PPP1CB and PPP1CC. Interacts also with its substrates ARHGEF2, BORA, BRCA1, KIF2A, PARD3, and p53/TP53. Interaction with BORA promotes phosphorylation of PLK1. Interacts with PIFO. Interacts with GADD45A, competing with its oligomerization. Interacts (via C-terminus) with AUNIP (via C-terminus). Identified in a complex with AUNIP and NIN. Interacts with FRY; this interaction facilitates AURKA-mediated PLK1 phosphorylation. Interacts with SIRT2. Interacts with MYCN; interaction is phospho-independent and triggers AURKA activation; AURKA competes with FBXW7 for binding to unphosphorylated MYCN but not for binding to phosphorylated MYCN. Interacts with HNRNPU. Interacts with AAAS. Interacts with KLHL18 and CUL3. Interacts with FOXP1.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
研究领域
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
文献引用
Application: WB Species: Human Sample:
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