RASSF2 Antibody - #DF8437

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产品: RASSF2 抗体
货号: DF8437
描述: Rabbit polyclonal antibody to RASSF2
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 37 kDa; 38kD(Calculated).
蛋白号: P50749
RRID: AB_2841683

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Xenopus(%)
克隆:
Polyclonal
特异性:
RASSF2 Antibody detects endogenous levels of total RASSF2.
RRID:
AB_2841683
引用格式: Affinity Biosciences Cat# DF8437, RRID:AB_2841683.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CENP 34; Centromere protein 34; Ras association (RalGDS/AF 6) domain family 2; Ras association (RalGDS/AF-6) domain family member 2; Ras association domain containing protein 2; Ras association domain-containing protein 2; RASF2_HUMAN; RASFADIN; RASSF2; RP4-599I11.1;

抗原和靶标

免疫原:
Uniprot:

P50749(RASF2_HUMAN) >>Visit HPA database.

基因/基因ID:
RASSF2(9770)
表达:
P50749 RASF2_HUMAN:

Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.

序列:
MDYSHQTSLVPCGQDKYISKNELLLHLKTYNLYYEGQNLQLRHREEEDEFIVEGLLNISWGLRRPIRLQMQDDNERIRPPPSSSSWHSGCNLGAQGTTLKPLTVPKVQISEVDAPPEGDQMPSSTDSRGLKPLQEDTPQLMRTRSDVGVRRRGNVRTPSDQRRIRRHRFSINGHFYNHKTSVFTPAYGSVTNVRINSTMTTPQVLKLLLNKFKIENSAEEFALYVVHTSGEKQKLKATDYPLIARILQGPCEQISKVFLMEKDQVEEVTYDVAQYIKFEMPVLKSFIQKLQEEEDREVKKLMRKYTVLRLMIRQRLEEIAETPATI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Bovine
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.

翻译修饰:

Phosphorylated by STK3/MST2 and STK4/MST1.

细胞定位:

Nucleus. Cytoplasm. Chromosome>Centromere>Kinetochore.
Note: Translocates to the cytoplasm in the presence of STK3/MST2 AND STK4/MST1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.

亚基结构:

Interacts directly with activated KRAS in a GTP-dependent manner. Interacts (via SARAH domain) with STK3/MST2 AND STK4/MST1.

研究领域

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

文献引用

1). MiR-93-5p promotes gastric cancer-cell progression via inactivation of the Hippo signaling pathway. GENE, 2018 (PubMed: 29045821) [IF=2.6]

Application: WB    Species: human    Sample:

Figure 4. FAT4 and LATS2 are direct miR-93-5p targets. (A) Quantitative PCR results showed that miR-93-5p overexpression suppressed FAT4 and LATS2 mRNA expression in SGC-7901 cells, while conversely, the opposite result was observed in HGC-27 cells exhibiting downregulated miR-93-5p expression. RUNX3 and RASSF2 mRNA levels were unchanged in response to either up- or downregulation of miR-93-5p. (B) Western blot analyses of FAT4, LATS2, RUNX3, and RASSF2 expression after transfection with miR-93-5p mimics and/or the miR-93-5p inhibitor.

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